Опубликована: Июнь 6, 2024

Thanks to several Vitis vinifera backcrosses with an initial V. L. × rotundifolia (previously Muscadinia rotundifolia) interspecific cross, the MrRUN1/MrRPV1 locus (resistance downy and powdery mildews) was introgressed in genotypes phenotypically close varieties. To check consequences of introgressing parts genome on gene expression during fruit development, we conducted a comparative RNA-seq study single berries from different cultivars hybrids, including ‘G5’ two derivative microvine lines, ‘MV102’ (resistant) ‘MV32’ (susceptible) segregating for MrRUN1/RPV1 locus. RNA-Seq profiles were analyzed comprehensive set end herbaceous plateau ripe stage. Pair-end reads aligned both PN40024.v4 reference genome, cv ‘Trayshed’ ‘Carlos’, few resistance genes original ‘52’ parent available at NCBI. Weighted Gene Co-expression Network Analysis (WGCNA) led classifying differentially expressed into 15 modules either preferentially correlated or berry phenology composition. Resistance positively transcripts predominantly mapped 4-5 Mb distal region chromosome 12 beginning locus, while negatively ones orthologous region, showing this large extremity LG12 remained recalcitrant internal recombination successive backcrosses. Some constitutively also observed lower density outside region. Genes overexpressed developing resistant accessions, rotundifolia, triggered by these spanned various functional groups, encompassing calcium signal transduction, hormone signaling, transcription factors, plant–pathogen-associated interactions, disease proteins, ROS phenylpropanoid biosynthesis. This transcriptomic insight provides foundation understanding inherent hybrid suggests constitutive NIR NBS LRR triggering signaling. Moreover, results illustrate magnitude changes caused background backcrossed number functions largely exceeding transformants.

Язык: Английский