Comparitive Chloroplast Genomics of Nine Endangered Habenaria Species and Phylogenetic Relationship of Orchidaceae DOI Open Access
Jinkui Zhang, Dangwei Zhou, Weidong Chen

и другие.

Опубликована: Июнь 10, 2024

Habenaria, a member of Orchidaceae family is the cosmopolitan distributions, which has significant medicinal and ornamental values. Regardless morphology molecular data that have been studied in recent times, phylogenetic relationship still under debate. Here, we sequenced, assembled, annotated whole chloroplast (cp) genome two species (Habenaria aitchisonii Rchb.f. Habenaria tibetica Schltr.ex Limpricht) grown on Qinghai-Tibetan Plateau (QTP), combined with seven already published cp genomes may assist to uncover their genomic profiling. The ranged from 155259-155269 bp length both encoded 132 genes, including 86 protein, 38 tRNA 8 rRNA. In genomes, tandem repeats (797), SSRs (2195) diverse loci (3214) were identified. Comparative analyses codon usage, amino frequency, microsatellite, oligo transition transversion substitutions showed similarities among species. Moreover, identified 16 highly polymorphic regions nucleotide diversity above 0.02, be suitable for robust authentic barcoding inferring phylogeny Among regions, positive selection was significantly exerted several genes such as cemA, petA, ycf1. This suggest important adaptation stratagem QTP. displayed H.aitchisonii H. closer than others rest clustered other three groups. Our findings also supported idea could divided into different sections. study enriched genomics resources helpful conservation efforts these endangered

Язык: Английский

Comparitive Chloroplast Genomics of Nine Endangered Habenaria Species and Phylogenetic Relationship of Orchidaceae DOI Open Access
Jinkui Zhang, Dangwei Zhou, Weidong Chen

и другие.

Опубликована: Июнь 10, 2024

Habenaria, a member of Orchidaceae family is the cosmopolitan distributions, which has significant medicinal and ornamental values. Regardless morphology molecular data that have been studied in recent times, phylogenetic relationship still under debate. Here, we sequenced, assembled, annotated whole chloroplast (cp) genome two species (Habenaria aitchisonii Rchb.f. Habenaria tibetica Schltr.ex Limpricht) grown on Qinghai-Tibetan Plateau (QTP), combined with seven already published cp genomes may assist to uncover their genomic profiling. The ranged from 155259-155269 bp length both encoded 132 genes, including 86 protein, 38 tRNA 8 rRNA. In genomes, tandem repeats (797), SSRs (2195) diverse loci (3214) were identified. Comparative analyses codon usage, amino frequency, microsatellite, oligo transition transversion substitutions showed similarities among species. Moreover, identified 16 highly polymorphic regions nucleotide diversity above 0.02, be suitable for robust authentic barcoding inferring phylogeny Among regions, positive selection was significantly exerted several genes such as cemA, petA, ycf1. This suggest important adaptation stratagem QTP. displayed H.aitchisonii H. closer than others rest clustered other three groups. Our findings also supported idea could divided into different sections. study enriched genomics resources helpful conservation efforts these endangered

Язык: Английский

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