Exploring Multifunctional Markers of Biological Age in Farmed Gilthead Sea Bream (<em>Sparus aurata</em>). A Transcriptomic and Epigenetic Interplay for an Improved Fish Welfare Assessment Approach DOI Open Access
Álvaro Belenguer, Fernando Naya-Català, Josep À. Calduch-Giner

и другие.

Опубликована: Июнь 17, 2024

DNA-methylation clocks inform not only about chronological but also biological age, which brings a high resolution and precise understanding of age-related pathology physiology. Attempts based on transcriptomic epigenetic approaches arise as integrative biomarkers linking the quantification stress response with given fitness trait may help to identify age markers, considered welfare indicators. In gilthead sea bream, targeted gene expression analyses in white skeletal muscle proved sirt1 reliable marker age-mediated changes energy metabolism. To complete list auditing biomarkers, wide-analyses one- three-year old fish were combined. After discriminant analysis, 668 differentially expressed transcripts matched those containing methylated (DM) regions (14,366), 172 overlapping. Through enrichment selection, two sets genes retained: 33 showing an opposite trend for expression, 57 down-regulated hypo-methylated. The first set displayed apparently more reproducible pattern 10 multifunctional DM CpG regulatory (sirt1, smad1, ramp1, psmd2 – up-regulated; col5a1, calcrl, bmp1, thrb, spred2, atp1a2 down-regulated) deemed candidate markers improved bream.

Язык: Английский

Exploring Multifunctional Markers of Biological Age in Farmed Gilthead Sea Bream (<em>Sparus aurata</em>). A Transcriptomic and Epigenetic Interplay for an Improved Fish Welfare Assessment Approach DOI Open Access
Álvaro Belenguer, Fernando Naya-Català, Josep À. Calduch-Giner

и другие.

Опубликована: Июнь 17, 2024

DNA-methylation clocks inform not only about chronological but also biological age, which brings a high resolution and precise understanding of age-related pathology physiology. Attempts based on transcriptomic epigenetic approaches arise as integrative biomarkers linking the quantification stress response with given fitness trait may help to identify age markers, considered welfare indicators. In gilthead sea bream, targeted gene expression analyses in white skeletal muscle proved sirt1 reliable marker age-mediated changes energy metabolism. To complete list auditing biomarkers, wide-analyses one- three-year old fish were combined. After discriminant analysis, 668 differentially expressed transcripts matched those containing methylated (DM) regions (14,366), 172 overlapping. Through enrichment selection, two sets genes retained: 33 showing an opposite trend for expression, 57 down-regulated hypo-methylated. The first set displayed apparently more reproducible pattern 10 multifunctional DM CpG regulatory (sirt1, smad1, ramp1, psmd2 – up-regulated; col5a1, calcrl, bmp1, thrb, spred2, atp1a2 down-regulated) deemed candidate markers improved bream.

Язык: Английский

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