Diversification of Pseudomonas aeruginosa After Inhaled Tobramycin Therapy of Cystic Fibrosis Patients: Genotypic and Phenotypic Characteristics of Paired Pre- and Post-Treatment Isolates

Microorganisms, Год журнала: 2025, Номер 13(4), С. 730 - 730

Опубликована: Март 24, 2025

This study examines the impact of inhaled tobramycin therapy on within-host changes in P. aeruginosa strains isolated from Bulgarian patients with CF prior to and post treatment. Genotypic comparison by RAPD-PCR indicated that most pre-treatment isolates had a high similarity were genetically comparatively close other countries known increased morbidity or treatment requirements. Most post-treatment were, however, distant their counterparts, showing genotypic diversification after Phenotypic comparisons showed lower ODmax reached during groswth an lag-time isolates. All capable invasion intracellular reproduction within A549 cultured cells. The addition sub-inhibitory amounts (1/4 1/2 MIC) growth higher relative fitness (as percentage untreated control) strains. effects sub-MICs biofilm did not show such pronounced trend. However, when resazurin-based viability test was applied, advantage confirmed for both broth cultures. In spite that, according determined MIC values, all tobramycin-sensitive, data this imply development tolerance antibiotic survived

Язык: Английский

Phage Therapy: An Alternative Approach to Combat Multi-drug Resistant Infections in Cystic Fibrosis

Опубликована: Июнь 21, 2024

In this review we aim to address the current status of phage therapy in management multidrug-resistant infections, from compassionate use cases ongoing clinical trials, as well challenges approach presents particular context CF patients.

Язык: Английский