Antifungal Synergy: Mechanistic Insights into the R-1-R Peptide and Bidens pilosa Extract as Potent Therapeutics against Candida spp. through Proteomics
Опубликована: Июль 4, 2024
It
was
previously
reported
that
the
peptide
derived
from
LfcinB,
R-1-R
exhibited
anti-Candida
activity
and
this
enhanced
in
combination
with
an
extract
Bidens
pilosa
plant.
However,
mechanism
of
action
has
not
been
studied.
In
current
study,
a
proteomic
study
carried
out,
followed
by
bioinformatic
analysis
biological
assays
both
SC5314
strain
fluconazole-resistant
isolate
Candida
albicans,
after
incubation
R-1-R.
The
data
showed
treatment
two
strains,
most
differentially
expressed
proteins
comparison
to
controls
were
up-regulated.
These
mainly
involved
membrane
cell
wall
biosynthesis,
transporters,
oxidative
stress,
mitochondrial
respiratory
chain,
response
DNA
damage.
Besides,
C.
albicans
parental
treated
ethanolic
B.
also
undertaken.
similar
functional
processes
as
alone,
but
at
different
levels
mostly
down-regulated.
allowed
validation
results,
evidencing
damage
surface,
generation
reactive
oxygen
species
decrease
potential.
results
provide
information
begin
unlock
understanding
complex
antifungal
mode
peptide,
its
extract,
possibly
other
derivatives
natural
products.
Язык: Английский
Special Issue “Antifungal Drug Discovery: Progresses, Challenges, Opportunities”
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(5), С. 2065 - 2065
Опубликована: Фев. 27, 2025
The
considerable
health
risks
associated
with
fungal
infections
are
continuously
rising,
thereby
requiring
proper
and
efficient
antifungal
treatment
options
[...].
Язык: Английский
Antiparasitic and Antifungal Activities of Cetyl-Maritima, a New N-Cetyl-Modified Maritima Derivative
Antibiotics,
Год журнала:
2025,
Номер
14(3), С. 321 - 321
Опубликована: Март 19, 2025
Background/Objectives:
New
drugs
are
urgently
needed
for
the
treatment
of
neglected
tropical
diseases
including
leishmaniasis
and
eumycetoma,
as
well
globally
occurring
parasitic
such
toxoplasmosis.
Fragrances,
both
natural
synthetic,
were
shown
to
be
a
rich
source
development
new
anti-infectives
warrant
deeper
investigations.
Exemplarily,
we
synthetically
optimized
fragrance
4-(4,8-dimethyl-3,7-nonadienyl)-pyridine,
a.k.a.
Maritima,
pyridine
derivative
with
marine
odor.
Methods:
A
cationic
N-cetyl-modified
Maritima
(dubbed
Cetyl-Maritima),
obtained
by
alkylation
was
tested
its
activity
against
Madurella
mycetomatis
(M.
mycetomatis)
fungi,
Toxoplasma
gondii
(T.
gondii)
Leishmania
major
(L.
major)
protozoal
parasites.
Results:
Cetyl-Maritima
found
more
strongly
antifungal
than
parent
known
antibiotic
cetylpyridinium
salt.
also
showed
similar
T.
parasites
and,
most
notably,
exhibited
sub-micromolar
L.
amastigotes.
Conclusions:
The
considerable
antileishmanial
might
lead
potent
cost-effective
drug
candidate
therapy
other
infectious
caused
kinetoplastid
Язык: Английский