Assessment of Immobilized Lacticaseibacillus rhamnosus OLXAL-1 Cells on Oat Flakes for Functional Regulation of the Intestinal Microbiome in a Type-1 Diabetic Animal Model DOI Creative Commons
Grigorios Nelios, Ioanna Prapa, Gregoria Mitropoulou

и другие.

Foods, Год журнала: 2024, Номер 13(24), С. 4134 - 4134

Опубликована: Дек. 20, 2024

The aim of this study was to examine the effect free or immobilized Lacticaseibacillus rhamnosus OLXAL-1 cells on oat flakes gut microbiota and metabolic inflammatory markers in a streptozotocin (STZ)-induced Type-1 Diabetes Mellitus (T1DM) animal model. Forty-eight male Wistar rats were assigned into eight groups (n = 6): healthy diabetic animals that received either control diet (CD DCD), an oat-supplemented (OD DOD), supplemented with L. (CFC DFC), (CIC DIC). Neither nor supplementation led any significant positive effects body weight, insulin levels, plasma glucose concentrations, lipid profile parameters. administration resulted rise relative abundances Lactobacillus Bifidobacterium, as well increased levels lactic, acetic, butyric acids feces animals. Additionally, significantly reduced microbial populations E. coli, Enterobacteriaceae, coliforms, staphylococci, enterococci lowered IL-1β blood These findings suggested probiotic food-based strategies could have potential therapeutic role managing dysbiosis inflammation associated T1DM.

Язык: Английский

A Novel Wild-Type Lacticaseibacillus paracasei Strain Suitable for the Production of Functional Yoghurt and Ayran Products DOI Creative Commons
Ioanna Prapa, Chrysoula Pavlatou, Vasiliki Kompoura

и другие.

Fermentation, Год журнала: 2025, Номер 11(1), С. 37 - 37

Опубликована: Янв. 17, 2025

Raw goat and ewe’s milk samples were used for the isolation of seven lactic acid bacteria new strains. After testing hemolytic activity resistance to antibiotics, specific functional properties evaluated; Lactococcus lactis subsp. FBM_1321 Lacticaseibacillus paracasei FBM_1327 strains resulted in highest cholesterol assimilation percentages ranging from 28.78 30.56%. In addition, strong adhesion capacity differentiated Caco-2 cells (1.77–21.04%) was mapped, lactobacilli exhibited antagonistic against foodborne pathogens compared lactococci. The able grow at low pH high NaCl concentrations, conditions that prevail food systems (cell counts ranged 1.77 8.48 log CFU/mL after exposure 3 5.66 9.52 concentrations up 8%). As a next step, freeze-dried immobilized Lc. on oat flakes preparation yoghurt ayran products. Cell loads strain remained stable both products (7.69 CFU/g 8.56 30 days storage 4 °C) throughout their shelf life. No significant changes volatile profile noticed, accepted by panel during sensory evaluation.

Язык: Английский

Процитировано

0

Effects of Free or Immobilized Pediococcus acidilactici ORE5 on Corinthian Currants on Gut Microbiome of Streptozotocin-Induced Diabetic Rat DOI Open Access
Ioanna Prapa, Vasiliki Kompoura, Chrysoula Pavlatou

и другие.

Опубликована: Авг. 20, 2024

Type-1 Diabetes Mellitus (T1D) onset and development is closely linked with imbalance of gut microbiome, while supplementation probiotics may result in positive alterations microbiome composition. In the present study, effect a dietary intervention including free or immobilized cells presumptive probiotic Pediococcus acidilactici ORE5 on Corinthian currants, food beneficial impact condition diabetes, was investigated. Male Wistar rats were studied which divided into 4 groups: healthy animals, received (H_FP) (H_IPC), diabetic (D_FP) (D_IPC), for weeks (109 cfu/day, all groups). At end intervention, D_IPC group exerted lower concentration inflammatory cytokine IL-1b compared to D_FP. Consumption currants P. by animals led increased loads fecal lactobacilli levels Enterobacteriaceae, coliforms, E. coli, Actinobacteria phylum, Akkermansia, Bifidobacterium genera abundances increased, lactic acid elevated. Overall, results research demonstrated that functional ingredients can ameliorate dysbiosis T1D could be used design patterns aiming at management. However, well-designed clinical trials are necessary, order confirm effects humans.

Язык: Английский

Процитировано

3

Assessment of Immobilized Lacticaseibacillus rhamnosus OLXAL-1 Cells on Oat Flakes for Functional Regulation of the Intestinal Microbiome in a Type-1 Diabetic Animal Model DOI Creative Commons
Grigorios Nelios, Ioanna Prapa, Gregoria Mitropoulou

и другие.

Foods, Год журнала: 2024, Номер 13(24), С. 4134 - 4134

Опубликована: Дек. 20, 2024

The aim of this study was to examine the effect free or immobilized Lacticaseibacillus rhamnosus OLXAL-1 cells on oat flakes gut microbiota and metabolic inflammatory markers in a streptozotocin (STZ)-induced Type-1 Diabetes Mellitus (T1DM) animal model. Forty-eight male Wistar rats were assigned into eight groups (n = 6): healthy diabetic animals that received either control diet (CD DCD), an oat-supplemented (OD DOD), supplemented with L. (CFC DFC), (CIC DIC). Neither nor supplementation led any significant positive effects body weight, insulin levels, plasma glucose concentrations, lipid profile parameters. administration resulted rise relative abundances Lactobacillus Bifidobacterium, as well increased levels lactic, acetic, butyric acids feces animals. Additionally, significantly reduced microbial populations E. coli, Enterobacteriaceae, coliforms, staphylococci, enterococci lowered IL-1β blood These findings suggested probiotic food-based strategies could have potential therapeutic role managing dysbiosis inflammation associated T1DM.

Язык: Английский

Процитировано

0