Development and application of a UHPLC-MS/MS method for the simultaneous determination of firmonertinib and its main metabolite AST-5902 in rat plasma: a study on the in vivo drug interaction between firmonertinib and paxlovid
Frontiers in Pharmacology,
Год журнала:
2025,
Номер
16
Опубликована: Май 12, 2025
Due
to
the
potential
occurrence
of
drug
interactions,
combined
application
firmonertinib
and
paxlovid
carries
a
relatively
high
risk.
Nevertheless,
as
now,
there
has
been
no
comprehensive
research
on
interaction
between
paxlovid.
Our
aim
was
establish
validate
an
accurate,
stable,
rapid
simple
UPLC-MS/MS
method
for
simultaneous
determination
its
metabolite
AST-5902
in
rat
plasma,
which
applied
study
vivo
Gefitinib
selected
internal
standard.
After
protein
precipitation
plasma
samples
with
acetonitrile,
separation
carried
out
Shimadzu
LC-20AT
UHPLC.
The
chromatographic
column
Shim-pack
Volex
PFPP
(50
mm
×
2.1
mm,
1.8
μm),
mobile
phase
composed
0.1%
formic
acid
-
water
methanol.
Mass
spectrometry
detection
performed
using
8,040
mass
spectrometer
ESI+
MRM
mode.
precision,
accuracy,
recovery
matrix
effect
this
were
detected.
linearity
stability
assessed.
Subsequently,
parent
ions
typical
fragment
firmonertinib,
IS
are
respectively
m/z
569.25
→
72.15,
555.50
498.10
447.25→
128.20.
selectivity,
specificity,
linearity,
recovery,
effect,
accuracy
precision
all
adequately
verified.
results
showed
that
when
paxlovid,
AUC
Cmax
significantly
increased,
while
AUC,
Tmax,
decreased.
established
UHPLC-MS/MS
is
simple.
Paxlovid
exhibit
significant
inhibitory
metabolism
rats.
Язык: Английский
COVID-19 and lung cancer
Pathologica,
Год журнала:
2023,
Номер
115(5), С. 284 - 291
Опубликована: Окт. 1, 2023
COVID-19
pandemic
had
affected
health
services
around
the
world,
also
reducing
diagnosis
of
lung
cancer.
On
other
hand,
examination
surgical
specimens
in
patients
with
cancer
and
SARS-CoV-2
gave
opportunity
to
evidence
early
histologic
features
related
this
emerging
pandemic.
Different
prioritization
organizations
during
resulted
a
significant
decline
screening
(up
56%),
delayed
30-40%)
higher
advanced
stage,
some
exceptions
(i.e.,
Canada).
Increased
use
stereotactic
radiation
treatments
stage
I-IIA
have
been
noticed
better-organized
systems.
Surgical
performed
for
incipient
SARSCoV-
2
permitted
appreciate
findings
hyperplastic
pneumocytes
with/without
fibrin
exudate,
alveolar
macrophages/myeloid
cells,
perivascular
T-lymphocytic
infiltrate
lack
hyaline
membrane.
While
has
declined
rate
worldwide,
institutions
significantly
limited
detrimental
effects.
Histology
infection
samples
revealed
specific
changes.
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reading
Язык: Английский