Mebendazole Exerts Anticancer Activity in Ovarian Cancer Cell Lines via Novel Girdin-Mediated AKT/IKKα/β/NF-κB Signaling Axis

Cells, Год журнала: 2025, Номер 14(2), С. 113 - 113

Опубликована: Янв. 14, 2025

Mebendazole (MBZ), a benzimidazole anthelmintic and cytoskeleton-disrupting compound, exhibits antitumor properties; however, its action on ovarian cancer (OC) is not clearly understood. This study evaluates the effect of MBZ OC cell lines OVCAR3 OAW42, focusing proliferation, migration, invasion, stemness. The underlying mechanisms, including cytoskeletal disruption, epithelial–mesenchymal transition (EMT), signaling pathways, were explored. inhibited OAW42 proliferation in dose- time-dependent manner. Additionally, significantly impedes spheroid colony formation, In addition, it reduced actin polymerization down-regulated CSC markers (e.g., CD24, CD44, EpCAM). Moreover, suppressed MMP-9 activity EMT marker as judged by decreased N-Cadherin Vimentin increased E-Cadherin. Furthermore, induced G2/M cycle arrest modulating Cyclin B1, CDC25C, WEE1. Also, triggered apoptosis disrupting mitochondrial membrane potential. Mechanistic studies revealed significant downregulation Girdin, an Akt modulator, along with p-Akt, p-IKKα/β, p-NF-κB, indicating MBZ’s novel mechanism through Girdin-mediated Akt/IKKα/β/NF-κB axis. Thus, targeting presents promising repurposed therapeutic strategy to inhibit metastasis cancer.

Язык: Английский

Computational Drug Repositioning Method Applied to Lung Cancer

IntechOpen eBooks, Год журнала: 2025, Номер unknown

Опубликована: Янв. 9, 2025

Lung cancer is the most common affecting men and women worldwide. Among different types of lung (LC), two primary forms stand out: small-cell (SCLC) non-small-cell (NSCLC). NSCLC, more prevalent variety, accounts for around 85–90% all cases. Drug repurposing, also known as drug repositioning, investigation using existing drugs new therapeutic purposes. This approach can lead to formulation effective treatments diseases that might not have been adequately addressed by traditional discovery methods. The importance repositioning in research cannot be overstated. Traditional often a lengthy costly process, requiring years substantial investment. In contrast, repurposing medications enhance pre-clinical journey developing drugs. High-performance computing capabilities enable efficient processing these extensive datasets, which may include biological, biomedical, electronic health-related information. data-driven has dramatically accelerated development computational methods repositioning. strategy offers promising pathway researchers, significantly shortening timeline reducing costs associated with development.

Язык: Английский

Transcriptome analysis displays new molecular insights into the mechanisms of action of Mebendazole in gastric cancer cells

Computers in Biology and Medicine, Год журнала: 2024, Номер 184, С. 109415 - 109415

Опубликована: Ноя. 19, 2024

Язык: Английский