Light-field tomographic fluorescence lifetime imaging microscopy DOI Creative Commons
Liang Gao, Yayao Ma, Luzhe Huang

и другие.

Research Square (Research Square), Год журнала: 2023, Номер unknown

Опубликована: Май 10, 2023

Abstract Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique that enables the visualization of biological samples at molecular level by measuring fluorescence decay rate fluorescent probes. This provides critical information about interactions, environmental changes, and localization within systems. However, creating high-resolution maps using conventional FLIM systems can be challenging, as it often requires extensive scanning significantly lengthen acquisition times. issue further compounded in three-dimensional (3D) because demands additional along depth axis. To tackle this challenge, we developed novel computational called light field tomographic (LIFT-FLIM). Our approach allows for volumetric images highly data-efficient manner, reducing number steps required compared to point-scanning or line-scanning imagers. Moreover, LIFT-FLIM measurement high-dimensional data low-dimensional detectors, which are typically low-cost feature higher temporal bandwidth. We demonstrated linear single-photon avalanche diode array on various systems, showcasing unparalleled detection sensitivity. Additionally, expanded functionality our method spectral its application high-content multiplexed lung organoids. has potential open up new avenues both basic translational biomedical research.

Язык: Английский

Fluorescence Lifetime Imaging Microscopy: Advances in Materials Science Research DOI
Fariyad Ali, Subhankar Kundu

Chemistry of Materials, Год журнала: 2025, Номер unknown

Опубликована: Май 27, 2025

Язык: Английский

Процитировано

0
Light-field tomographic fluorescence lifetime imaging microscopy DOI Creative Commons
Liang Gao, Yayao Ma, Luzhe Huang

и другие.

Research Square (Research Square), Год журнала: 2023, Номер unknown

Опубликована: Май 10, 2023

Abstract Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique that enables the visualization of biological samples at molecular level by measuring fluorescence decay rate fluorescent probes. This provides critical information about interactions, environmental changes, and localization within systems. However, creating high-resolution maps using conventional FLIM systems can be challenging, as it often requires extensive scanning significantly lengthen acquisition times. issue further compounded in three-dimensional (3D) because demands additional along depth axis. To tackle this challenge, we developed novel computational called light field tomographic (LIFT-FLIM). Our approach allows for volumetric images highly data-efficient manner, reducing number steps required compared to point-scanning or line-scanning imagers. Moreover, LIFT-FLIM measurement high-dimensional data low-dimensional detectors, which are typically low-cost feature higher temporal bandwidth. We demonstrated linear single-photon avalanche diode array on various systems, showcasing unparalleled detection sensitivity. Additionally, expanded functionality our method spectral its application high-content multiplexed lung organoids. has potential open up new avenues both basic translational biomedical research.

Язык: Английский

Процитировано

4