Floral phenotypic divergence and genomic insights in anOphrysorchid: Unraveling early speciation processes

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Март 23, 2024

Summary Adaptive radiation in Ophrys orchids leads to complex floral phenotypes that vary scent, color and shape. Using a novel pipeline quantify these phenotypes, we investigated trait divergence at early stages of speciation six populations aveyronensis experiencing recent allopatry. By integrating different genetic/genomic techniques, investigated: (i) variation integration components (scent, shape), (ii) genomic regions under divergent selection, (iii) the bases variation. We identified large island divergence, associated with phenotypic particular odor. detected potential selection on macular color, while convergent was suspected morphology for several volatile olfactive compounds. also identify candidate genes involved anthocyanin steroid biosynthesis pathways standing genetic This study sheds light differentiation , revealing patterns often become invisible over time, i.e., geographic mosaic traits appearance strong divergence. It supports crucial region future investigation highlights value multifaceted approach unraveling within taxa genomes.

Язык: Английский

Contrasting patterns of differentiation among three taxa of the rapidly diversifying orchid genusOphryssect.Insectifera(Orchidaceae) where their range overlap

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Апрель 27, 2024

ABSTRACT In rapidly diversifying groups, taxa defined on the basis of typological criteria can be difficult to support with genetic data. The diversity observed in insect-mimicking orchid genus Ophrys perfectly illustrates this situation; among 400 described species only 9-10 lineages are detectable by markers such as nrITS. three insectifera group: O. , subinsectifera and aymoninii clearly distinguished their flowers, which have evolved different phenotypes a result adaptation specific pollinator insect from families. However, differentiation between these has never been really supported population data taxonomic status is still debated. Using genomic approaches, we found clustering consistent existence entities where geographic distributions overlap. Two clusters correspond France populations widespread micro-endemic . last cluster grouped together all Iberian individuals, suggesting that individuals phenotypically identified either or genetically weakly differentiated there. Populations two pairs thus may experienced patterns inter-specific gene flow.

Язык: Английский

Orchidinae‐205: A new genome‐wide custom bait set for studying the evolution, systematics, and trade of terrestrial orchids

Molecular Ecology Resources, Год журнала: 2024, Номер 24(6)

Опубликована: Июнь 20, 2024

Abstract Terrestrial orchids are a group of genetically understudied, yet culturally and economically important plants. The Orchidinae tribe contains many species that produce edible tubers used for the production traditional delicacies collectively called ‘salep’. Overexploitation wild in Eastern Mediterranean Western Asia threatens to drive these extinction, but cost‐effective tools monitoring their trade currently lacking. Here we present custom bait kit target enrichment sequencing 205 novel genetic markers tailored phylogenomic applications s.l. A subset 31 capture genes putatively involved glucomannan, water‐soluble polysaccharide gives salep its distinctive properties. We tested on 73 taxa native area, demonstrating universally high locus recovery irrespective identity, exceeds total sequence length obtained with alternative kits available. Phylogenetic inference concatenation coalescent approaches was robust showed levels support most clades, including some which were previously unresolved. Resolution hybridizing recently radiated lineages remains difficult, could be further improved by analysing multiple haplotypes non‐exonic sequences captured our kit, promise shed new light evolution enigmatic complex speciation history. Offering step‐up from barcoding universal markers, genome‐wide loci targeted Orchidinae‐205 valuable resource study evolution, systematics terrestrial orchids.

Язык: Английский