Long term co-circulation of multiple arboviruses in southeast Australia revealed by xeno-monitoring of mosquitoes and metatranscriptomics DOI
Carla Júlia da Silva Pessoa Vieira, Michael B. Onn, Martin A. Shivas

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Март 30, 2024

Abstract Arbovirus surveillance of wild-caught mosquitoes is an affordable and sensitive means monitoring virus transmission dynamics at various spatial-temporal scales, emergence re-emergence during epidemic interepidemic periods. A variety molecular diagnostics for arbovirus screening (known as xeno-monitoring) are available, but most provide limited information about diversity. PCR-based coupled with metatranscriptomics increasingly pipeline integrating complete viral genome sequencing into programs. This enables large-scale, high-throughput from diverse samples. We collected in CO2-baited light traps five urban parks Brisbane March 2021 to May 2022. Mosquito pools ≤200 specimens were screened alphaviruses flaviviruses using genus-specific primers reverse transcription quantitative PCR (qRT-PCR). subset virus-positive samples was then processed a mosquito-specific ribosomal RNA depletion method sequenced on the Illumina NextSeq. Overall, 54,670 mosquitoes, representing 26 species 382 pools. Thirty detections arboviruses made 28 Twenty these positive further characterised meta-transcriptomics generating 18 full-length genomes. These sequences belonged four medically relevant arboviruses: Barmah Forest, Ross River, Sindbis-like Stratford viruses. Phylogenetic evolutionary analyses revealed progression lineages over last 100 years, highlighting long-distance dispersal across Australian continent continuous circulation by constant turnover lineages.

Язык: Английский

Long term co-circulation of multiple arboviruses in southeast Australia revealed by xeno-monitoring of mosquitoes and metatranscriptomics DOI
Carla Júlia da Silva Pessoa Vieira, Michael B. Onn, Martin A. Shivas

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Март 30, 2024

Abstract Arbovirus surveillance of wild-caught mosquitoes is an affordable and sensitive means monitoring virus transmission dynamics at various spatial-temporal scales, emergence re-emergence during epidemic interepidemic periods. A variety molecular diagnostics for arbovirus screening (known as xeno-monitoring) are available, but most provide limited information about diversity. PCR-based coupled with metatranscriptomics increasingly pipeline integrating complete viral genome sequencing into programs. This enables large-scale, high-throughput from diverse samples. We collected in CO2-baited light traps five urban parks Brisbane March 2021 to May 2022. Mosquito pools ≤200 specimens were screened alphaviruses flaviviruses using genus-specific primers reverse transcription quantitative PCR (qRT-PCR). subset virus-positive samples was then processed a mosquito-specific ribosomal RNA depletion method sequenced on the Illumina NextSeq. Overall, 54,670 mosquitoes, representing 26 species 382 pools. Thirty detections arboviruses made 28 Twenty these positive further characterised meta-transcriptomics generating 18 full-length genomes. These sequences belonged four medically relevant arboviruses: Barmah Forest, Ross River, Sindbis-like Stratford viruses. Phylogenetic evolutionary analyses revealed progression lineages over last 100 years, highlighting long-distance dispersal across Australian continent continuous circulation by constant turnover lineages.

Язык: Английский

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