The collaborative cross mouse for studying the effect of host genetic background on memory impairments due to obesity and diabetes DOI Creative Commons

Avia Paz,

Kareem Midlej, Osayd Zohud

и другие.

Animal Models and Experimental Medicine, Год журнала: 2024, Номер unknown

Опубликована: Окт. 28, 2024

Abstract Background Over the past few decades, a threefold increase in obesity and type 2 diabetes (T2D) has placed heavy burden on health‐care system society. Previous studies have shown correlations between obesity, T2D, neurodegenerative diseases, including dementia. It is imperative to further understand relationship cognitive deficits. Methods This investigation tested evaluated impact of T2D induced by high‐fat diet (HFD) effect host genetic background severity decline caused collaborative cross (CC) mice. The CC mice are genetically diverse panel derived from eight inbred strains. Results Our findings demonstrated significant variations recorded phenotypes across different lines compared reference mouse line, C57BL/6J. CC037 line exhibited substantial body weight HFD, whereas CC005 differing responses based sex. Glucose tolerance tests revealed variations, with some like showing marked area under curve (AUC) values HFD. Organ weights, brain, spleen, liver, kidney, varied significantly among sexes response Behavioral using Morris water maze indicated that performance was differentially affected background. Conclusions study establishes foundation for future quantitative trait loci mapping identifying genes underlying comorbidity Alzheimer's disease (AD), T2D. components may offer new tools early prediction prevention.

Язык: Английский

System genetic analysis of intestinal cancer and periodontitis development as influenced by aging and diabesity using Collaborative Cross mice DOI Creative Commons
Iqbal M. Lone, Osayd Zohud, Kareem Midlej

и другие.

Animal Models and Experimental Medicine, Год журнала: 2025, Номер unknown

Опубликована: Фев. 7, 2025

It is increasingly recognized that young, chow-fed inbred mice poorly model the complexity of human carcinogenesis. In humans, age and adiposity are major risk factors for malignancies, but most genetically engineered mouse models (GEMM) induce carcinogenesis too rapidly to study these influences. Standard strains, such as C57BL/6, commonly used in GEMMs, further limit exploration aging metabolic health effects. A similar challenge arises modeling periodontitis, a disease influenced by aging, diabesity, genetic architecture. We propose using diverse populations with hybrid vigor, Collaborative Cross (CC) × ApcMin hybrid, slow progression better colorectal cancer (CRC) comorbidities. This perspective highlights advantages this model, where delayed reveals interactions adiposity. Unlike mice, which develop rapidly, CC hybrids recapitulate human-like progression. facilitates identification modifier loci affecting inflammation, diet susceptibility, organ size, polyposis distribution. The offers transformative platform studying CRC adulthood, reflecting its complex interplay insights gained from approach will enhance early detection, management, treatment strategies related conditions.

Язык: Английский

Процитировано

0

The collaborative cross mouse for studying the effect of host genetic background on memory impairments due to obesity and diabetes DOI Creative Commons

Avia Paz,

Kareem Midlej, Osayd Zohud

и другие.

Animal Models and Experimental Medicine, Год журнала: 2024, Номер unknown

Опубликована: Окт. 28, 2024

Abstract Background Over the past few decades, a threefold increase in obesity and type 2 diabetes (T2D) has placed heavy burden on health‐care system society. Previous studies have shown correlations between obesity, T2D, neurodegenerative diseases, including dementia. It is imperative to further understand relationship cognitive deficits. Methods This investigation tested evaluated impact of T2D induced by high‐fat diet (HFD) effect host genetic background severity decline caused collaborative cross (CC) mice. The CC mice are genetically diverse panel derived from eight inbred strains. Results Our findings demonstrated significant variations recorded phenotypes across different lines compared reference mouse line, C57BL/6J. CC037 line exhibited substantial body weight HFD, whereas CC005 differing responses based sex. Glucose tolerance tests revealed variations, with some like showing marked area under curve (AUC) values HFD. Organ weights, brain, spleen, liver, kidney, varied significantly among sexes response Behavioral using Morris water maze indicated that performance was differentially affected background. Conclusions study establishes foundation for future quantitative trait loci mapping identifying genes underlying comorbidity Alzheimer's disease (AD), T2D. components may offer new tools early prediction prevention.

Язык: Английский

Процитировано

0