Belling the “cat”: Wnt/β-catenin signaling and its significance in future cancer therapies

Biochimica et Biophysica Acta (BBA) - Reviews on Cancer, Год журнала: 2024, Номер unknown, С. 189195 - 189195

Опубликована: Окт. 1, 2024

Язык: Английский

---

Role of the Wnt and GTPase pathways in breast cancer tumorigenesis and treatment

Cytokine & Growth Factor Reviews, Год журнала: 2022, Номер 67, С. 11 - 24

Опубликована: Май 4, 2022

Язык: Английский

---

Antisense mediated blockade of Dickkopf 1 attenuates tumor survival, metastases and bone damage in experimental osteosarcoma

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Янв. 13, 2025

Osteosarcoma (OS) is the most common primary bone malignancy. The canonical Wnt inhibitor Dickkopf-1 (Dkk-1) has been implicated in destruction, tumor survival and metastases during OS. We examined role of Dkk-1 OS disease progression explored strategies for targeting its activity. enhances by amplifying a non-canonical pathway that upregulates aldehyde dehydrogenase 1A1. Targeting transcription with vivo morpholino (DkkMo) reduced enhanced osteogenic activity vitro. DkkMo as single agent slowed expansion, increased necrosis, inhibited preserved PDX model also frequency dividing cells reinitiated regenerative phenotype tumors stroma while reducing infiltration inflammatory cells. These findings indicate potential to safely target osteosarcoma growth, survival, destruction.

Язык: Английский

---

Differential Signaling Pathways Identified in Aqueous Humor, Anterior Capsule, and Crystalline Lens of Age-Related, Diabetic, and Post-Vitrectomy Cataract

Proteomes, Год журнала: 2025, Номер 13(1), С. 7 - 7

Опубликована: Фев. 3, 2025

Background: The purpose of this study was to detect proteomic alterations and corresponding signaling pathways involved in the formation age-related cataract (ARC), diabetic (DC), post-vitrectomy (PVC). Methods: Three sample types, aqueous humor (AH), anterior capsule (AC), content phaco cassette, were collected during phacoemulsification surgery. samples obtained from 12 participants without diabetes mellitus (DM), 11 with DM, 7 a history vitrectomy surgery past months. Sp3 protocol (Single-Pot, Solid-Phase, Sample-Preparation) used for preparation. recognition quantification proteins carried out liquid chromatography online tandem mass spectrometry. DIA-NN software applied identification peptides/proteins. Statistical analysis data visualization conducted on Perseus software. Data are available via ProteomeXchange. Results: A very rich atlas lens AH proteome has been generated. Glycosaminoglycan biosynthesis non-canonical Wnt receptor pathway differentially expressed ARC compared both DC PVC groups. In group, complement activation samples, while glutathione metabolism oxidoreductase activity AC samples. Microfilament motor activity, microtubule cytoskeleton organization, binding groups Conclusions: results expand existing knowledge pathophysiology cataract, suggest possible important druggable targets slower progression or even prevention cataract.

Язык: Английский

---

An update on cancer stem cell survival pathways involved in chemoresistance in triple-negative breast cancer

Future Oncology, Год журнала: 2025, Номер unknown, С. 1 - 21

Опубликована: Фев. 12, 2025

Triple-negative breast cancer (TNBC) presents a formidable global health challenge, marked by its aggressive behavior and significant treatment resistance. This subtype, devoid of estrogen, progesterone, HER2 receptors, largely relies on stem cells (BCSCs) for progression, metastasis, recurrence. BCSCs, characterized their self-renewal capacity resistance to conventional therapies, exploit key surface markers critical signaling pathways like Wnt, Hedgehog, Notch, TGF-β, PI3K/AKT/mTOR Hippo-YAP/TAZ thrive. Their adaptability is underscored mechanisms including drug efflux enhanced DNA repair, contributing poor prognosis high recurrence rates. The tumor microenvironment (TME) further facilitates BCSC survival through complex interactions with stromal immune cells. Emerging therapeutic strategies targeting BCSCs - ranging from immunotherapy nanoparticle-based delivery systems gene-editing technologies aim disrupt these resistant Additionally, innovative approaches focusing exosome-mediated metabolic reprogramming show promise in overcoming chemoresistance. By elucidating the distinct characteristics role TNBC, researchers are paving way novel treatments that may effectively eradicate resilient cells, mitigate ultimately improve patient outcomes. review highlights urgent need targeted address unique biology pursuit more effective interventions TNBC.

Язык: Английский

---

CtBP2 Regulates Wnt Signal Through EGR1 to Influence the Proliferation and Apoptosis of DLBCL Cells

Molecular Carcinogenesis, Год журнала: 2025, Номер unknown

Опубликована: Март 18, 2025

ABSTRACT Diffuse large B‐cell lymphoma (DLBCL) is the most prevalent form of lymphoma. The overexpression CtBP2 in tissues may contribute to tumor occurrence and progression. expression EGR1 DLBCL elevated, suggesting its potential role as an oncogene that promotes proliferation cells. Database predictions indicate can bind EGR1. objective present study was investigate whether influence apoptosis cells by regulating Wnt signaling pathway through Western blot assay showed upregulated Cell level detected CCK8 EdU staining, cycle distribution were analyzed flow cytometry. Our data indicated interference with inhibit cell progression while promoting apoptosis. from HDOCK server, along results Co‐IP experiments, suggested effectively each other, positioned downstream regulated it. Furthermore, could also Wnt/β‐catenin pathway. Overexpression counteracted effects siRNA‐CtBP2, cycle, inhibiting upregulating From above we found regulate Therefore, be one key contributors involved regulation CtBP2.

Язык: Английский

---

Role and Therapeutic Implication of Wnt Signalling Pathway in Cancer

Опубликована: Янв. 1, 2025

Язык: Английский

---

PYGO2 regulates IL10 and plays immunosuppressive role through ESCC progression

BMC Molecular and Cell Biology, Год журнала: 2025, Номер 26(1)

Опубликована: Апрель 29, 2025

Esophageal squamous cell carcinoma (ESCC), one of the most aggressive carcinomas gastrointestinal tract, is sixth common cause cancer-related death. Wnt pathway plays a pivotal role in proliferation and differentiation. PYGO2 IL10 are involved this pathway. Our aim study was to examine correlation between expression ESCC patients lines. Relative-comparative real time-PCR (RT-qPCR) used evaluate mRNA profile 58 non-treated compared their margin normal tissues. Expression induced KYSE-30 YM1 lines analyzed. The results revealed significant overexpression 31.0% 51.7% ESCCs, respectively. significantly correlated each other (p = 0.007). Concomitant genes associated grade tumor differentiation < 0.01), depth invasion 0.05). Induced caused meaningful change cells. may regulate through Wnt/β-catenin signaling pathway, suggesting possible oncogenic for PYGO2/IL10 axis tumorigenesis. Considering involvement as an anti-inflammatory cytokine elevated metastasis, functional interaction these factors promote process which induces malignant phenotype ESCC.

Язык: Английский

---

NOVA1 promotes NSCLC proliferation and invasion by activating Wnt/β-catenin signaling

BMC Cancer, Год журнала: 2022, Номер 22(1)

Опубликована: Окт. 25, 2022

Neuro-oncological ventral antigen 1 (NOVA1) is a neuron-specific RNA-binding protein which regulates alternative splicing in the developing nervous system. Recent research has found that NOVA1 plays significant role carcinogenesis. In this paper, we examine of non-small cell lung cancer (NSCLC) and its underlying molecular mechanisms.The expression NSCLC was detected by immunohistochemistry correlations between clinicopathological factors were analyzed chi-square tests. Kaplan-Meier survival analysis Cox regression model used to evaluate predictive effect prognostic factors. Western blotting, Cell Counting Kit-8, colony formation, apoptosis, migration invasion assays detect effects silencing (si)NOVA1 RNA on Wnt/β-catenin signaling biological behavior lines.Our study showed up-regulated significantly correlated with poor differentiation (p = 0.020), advanced TNM stage (P 0.001), T 0.001) lymph node metastasis 0.000) as well β-catenin 0.012) NSCLC. The down-regulation siRNA inhibited proliferation, promoted apoptosis cells. Expression Wnt molecules, including β-catenin, activated cyclin D1, matrix metalloproteinase (MMP)-2 MMP-7, also reduced siNOVA1. inhibition A549 H1299 cells siNOVA1 reversed after treatment plasmid.The present suggests may serve potential prognosis biomarker High associated rate. Finally, vitro experiments verified promotes proliferation regulating signaling.

Язык: Английский

---

LINC01189-miR-586-ZEB1 feedback loop regulates breast cancer progression through Wnt/β-catenin signaling pathway

Molecular Therapy — Nucleic Acids, Год журнала: 2021, Номер 25, С. 455 - 467

Опубликована: Июнь 24, 2021

Non-coding RNAs play essential roles in breast cancer progression by regulating proliferation, differentiation, invasion, and metastasis. However, our understanding of most microRNAs (miRNAs) long noncoding (lncRNAs) is still limited. miR-586 has been identified as an important factor the some types cancer, but its exact function relative regulation mechanisms development need to be further investigated. In this study, we showed functioned oncogene promoting proliferation metastasis both vitro vivo. Meanwhile, induced Wnt/β-catenin activation directly targeting signaling antagonists SFRP1 DKK2/3. Moreover, demonstrated that LINC01189 a tumor suppressor inhibited through inhibiting epithelial-mesenchymal transition (EMT)-like phenotype sponging miR-586. addition, β-catenin/TCF4 transactivated ZEB1, resulting transcriptional repression expression. conclusion, data uncovered LINC01189-miR-586-ZEB1 feedback loop provided novel mechanism participating progression.

Язык: Английский

---