Antioxidants,
Год журнала:
2023,
Номер
12(2), С. 377 - 377
Опубликована: Фев. 4, 2023
CHIR99021
is
an
aminopyrimidine
derivative,
which
can
efficiently
inhibit
the
activity
of
glycogen
synthesis
kinase
3α
(GSK-3α)
and
GSK-3β.
As
essential
component
stem
cell
culture
medium,
it
plays
important
role
in
maintaining
stemness.
However,
mechanism
its
not
fully
understood.
In
present
study,
we
first
found
that
removal
from
embryonic
medium
reduced
iron
storage
mouse
cells
(mESCs).
CHIR99021-treated
Neuro-2a
led
to
upregulation
ferritin
expression
increase
intracellular
levels,
along
with
GSK3β
inhibition
Wnt/GSK-3β/β-catenin
pathway
activation.
addition,
treatment
activated
classical
Wnt
by
affecting
β-catenin
cells.
Our
data
link
maintenance
stemness
via
signaling
pathway,
identify
intermediate
molecules,
including
Steap1,
Bola2,
Kdm6bos,
may
mediate
CHIR99021.
These
findings
reveal
novel
mechanisms
differentiation
provide
a
theoretical
basis
for
development
new
strategies
disease.
Mucosal Immunology,
Год журнала:
2024,
Номер
17(4), С. 752 - 767
Опубликована: Июнь 12, 2024
Serving
as
a
part
of
intestinal
innate
immunity,
Paneth
cells
play
an
important
role
in
homeostasis
maintenance
via
their
multiple
functions.
However,
the
regulation
has
been
proven
to
be
complex
and
diverse.
Here,
we
identified
nuclear
receptor
Nur77
novel
regulator
cell
differentiation
function.
deficiency
led
loss
murine
ileal
crypts.
Intestinal
tissues
or
organoids
with
exhibited
impaired
stem
niche
failed
enhance
antimicrobial
peptide
expression
after
degranulation.
The
defects
peptides
Nur7−/−
mice
microbiota
disorders.
rendered
postnatal
susceptible
necrotizing
enterocolitis.
Mechanistically,
transcriptionally
inhibited
Dact1
activate
Wnt
signaling
activity,
thus
promoting
Taken
together,
our
data
suggest
regulatory
function
reveal
Dact1-mediated
inhibition
mechanism
development.
