The potential use of bacteriophages as antibacterial agents against Klebsiella pneumoniae

Virology Journal, Год журнала: 2024, Номер 21(1)

Опубликована: Авг. 19, 2024

One of the most common bacteria that cause nosocomial infections is Klebsiella pneumonia (K. pneumoniae), especially in patients who are very sick and admitted to intensive care unit (ICU). The frequency multi-drug-resistant pneumoniae (MDRKP) has dramatically increased worldwide recent decades, posing an urgent threat public health. Western world's bacteriophage (phage) studies have been revitalized due increasing reports antimicrobial resistance restricted development discovery new antibiotics. These factors also spurred innovation other scientific domains. primary agent phage treatment obligately lytic organism (called bacteriophage) kills corresponding bacterial host while sparing human cells lessening broader effects antibiotic usage on commensal bacteria. Phage developing quickly, leading many clinical instances life-saving medicinal use. In addition, a few immunological adverse consequences addition its usefulness. Since K. made treating multidrug-resistant (MDR) challenging, therapy (PT) emerged as novel therapeutic strategy. effectiveness phages investigated biofilms animal infection models. Compared with antibiotics, PT exhibits numerous advantages, including particular lysis spectrum, co-evolution avoid emergence resistance, higher abundance diversity resources than found Moreover, eliminated absence bacterium, which makes them only self-regulates at sites infection. Therefore, it essential pay attention role these infections. This study summarizes state knowledge spp. provides outlook phage-based treatments target trials.

Язык: Английский

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Altitudinal influence on survival mechanisms, nutritional composition, and antimicrobial activity of Moringa Peregrina in the summer climate of Fujairah, UAE

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Фев. 15, 2025

Extreme environments significantly impact the metabolic profiles of plants, leading to variations in chemical composition and bioactivity. This study investigates effects altitude, plant part age, light exposure on antimicrobial properties Moringa peregrina. Based our results, mineral contents were location dependent; while dry matter, ash, total digestible nutrients (TDN), fibre, protein, tannins, greater samples from mountain. Vitamin E was more concentrated wadi. A, selenium, phenols, heavy metals undetectable both environments. Antimicrobial assays revealed stronger activity mountain than other locations. Age-dependent analyses showed that nitrogen, electrolyte leakage higher young parts, whereas chlorophyll a b levels elevated mature leaves younger branches, particularly lower elevations. Interestingly, regions had concentrations compared leaves, contrasting with trends at Photosynthetic photon flux density (PPFD) measurements also highest region. These findings suggest M. peregrina employs diverse adaptations for survival challenging environments, potentially offering socioeconomic value indigenous communities through its bioactive properties.

Язык: Английский

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The metagenomic and whole-genome metagenomic detection of multidrug-resistant bacteria from subclinical mastitis-affected cow’s milk in India

Frontiers in Cellular and Infection Microbiology, Год журнала: 2025, Номер 15

Опубликована: Апрель 22, 2025

Multidrug-resistant (MDR) bacteria in farm animal products threaten human health by causing zoonotic infections. The complete genome sequences of MDR from subclinical mastitis-affected cow’s milk have not yet been comprehensively documented India. In this study, using a bacterial metagenomic sequencing approach, we detected the nearly Pseudomonas veronii and Staphylococcus xylosu s milk. Furthermore, sequenced Escherichia coli , Klebsiella pneumoniae hominis S. xylosus whole-genome method. Our analysis subsequently revealed that identified contained genes for multiple virulence factors. These may pose public risk through exposure to milkers, handlers, workers or handling consumption unpasteurized

Язык: Английский

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Klebsiella pneumoniae capsular polysaccharide: Mechanism in regulation of synthesis, virulence, and pathogenicity

Virulence, Год журнала: 2024, Номер 15(1)

Опубликована: Дек. 13, 2024

Hypervirulent Klebsiella pneumoniae exhibits strong pathogenicity and can cause severe invasive infections but is historically recognized as antibiotic-susceptible. In recent years, the escalating global prevalence of antibiotic-resistant hypervirulent K. has raised substantial concerns created an urgent demand for effective treatment options. Capsular polysaccharide (CPS) one main virulence determinants contributing to phenotype. The structure CPS varies widely among strains, both composition influence pneumoniae. possesses various immune evasion mechanisms that promote survival pneumoniae, well its colonization dissemination. Given proven viability therapies target capsule, improving our understanding critical effectively directing strategies. this review, typing are addressed genes related synthesis regulation, relationships with virulence, pathogenic mechanisms. We aim provide a reference research on pathogenesis

Язык: Английский

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Transcriptomic analysis reveals pathways underlying the multi‐antibiotic resistance of Klebsiella pneumoniae

IET Systems Biology, Год журнала: 2024, Номер unknown

Опубликована: Дек. 17, 2024

Klebsiella pneumoniae, an opportunistic pathogen, is pervasively distributed across the world. Its escalating antibiotic resistance poses a serious threat to global public health. The mechanisms behind this remain largely elusive. In study, we performed susceptibility testing on several clinical isolates of and reference strain ATCC13883, then analysed their transcriptomic profiles identify genes pathways associated with resistance. Our results showed that isolate DY16KPN may counteract antibiotics by enhancing biosynthesis building blocks bacterial cell, such as fatty acids, proteins, DNA, reducing transmembrane transport. Increased butanoate metabolism lipopolysaccharide also contribute drug-resistance pneumoniae. Additionally, identified resistance-promoting mutations in gene promoter regions, which regulate activity downstream drug-resistant pathways. demonstrated counterbalances trimethoprim/sulfamethoxazole-mediated inhibitory effect synthesis tetrahydrofolates DNA up-regulating expression targeted enzymes trimethoprim/sulfamethoxazole, dihydrofolate reductase dihydropteroate synthase.

Язык: Английский

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Klebsiella pneumoniae ST258 impairs intracellular elastase mobilization and persists within human neutrophils

Microbiological Research, Год журнала: 2024, Номер 292, С. 128035 - 128035

Опубликована: Дек. 26, 2024

Язык: Английский

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