Phytochemical constituent analysis, antioxidative activity, and cytotoxic effects of Caulerpa lentillifera extracts inducing cell apoptosis and sub-G/G0-G1 cell cycle arrest in KON oral cancer cells

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Дек. 9, 2024

Background The purpose of this study was to explore the anti-cancer properties Caulerpa lentillifera (C. lentillifera) in order gain insights into possible treatment approaches. Using maceration method, which included solubilizing chemicals such as methanol, ethanol, and acetone, C. extracted. chemical composition acetone extracts investigated their anti-oxidative activity evaluated. Methods phytoconstituents were screened using LC-QTOF MS/MS technique, cytotoxic effect assessed MTT test. We used Hoechst 33258 AO double staining investigate morphological changes apoptosis, DAPI FDA labeling look at nucleus cytoplasm. a flow cytometer, percentage cell cycle arrest calculated fraction death examined. Results analyses results revealed ten main phytochemicals extracts. KON cells exhibited sensitivity methanol that order. By causing ROS-mediated death, concentrations extract IC₄₀, IC₆₀, IC₈₀ can cause apoptosis less than day. prevented from advancing past sub-G G0-G1 stages by all three Furthermore, significantly impeded colony growth, migration, invasion. increase cellular uptake measured TEER Conclusion This work advances our understanding anti-tumor characteristics extract, will benefit patients suffering oral cancer.

Язык: Английский

An in vitro anticancerous and antioxidant potentials of the brown seaweed Sargassum polycystum C. Agardh

Kongunadu Research Journal, Год журнала: 2023, Номер 10(2), С. 31 - 34

Опубликована: Дек. 31, 2023

Discovering new therapeutic agents for cancer treatment remains a significant issue in the search cure as, is preeminent reason of death worldwide. The majority compounds used as chemotherapeutic medications to treat have been found and isolated plants their synthetic derivatives. Biomaterials made from marine algae are crucial components several other diseases, due diverse bioactivities. goal current study was assess Sargassum polycystum antioxidant capacity anticancer efficacy against A549 cell line. antioxidants preventing oxidative stress-related damage (OS). OS has linked pathogenesis illnesses, including cancer, diabetes, heart disease. Marine algae-derived natural shield cells by reducing effects stress. From algal extract DPPH radical scavenging activity concentration–dependent manner with maximum (IC50 value = 27.7±1.3µg/ml) carried out. vitro anticancerous Lung line revealed that IC50 13±1.5 µg/ml. Thus we can deduce secondary metabolites advance substantial range anti-cancerous medicaments.

Язык: Английский