Fe-capsaicin nanozyme attenuates sepsis-induced acute lung injury by regulating the functions of macrophages DOI Creative Commons

Ruijie Wang,

Ning Zhou,

Jinfang Xue

и другие.

Frontiers in Bioengineering and Biotechnology, Год журнала: 2024, Номер 12

Опубликована: Ноя. 18, 2024

Background In sepsis, the lung is one of worst affected organs, often leading to acute injury (ALI). More and more evidence suggests that macrophages are also involved in pathogenesis ALI. our previous study, we successfully synthesized Iron-capsaicin-based nanoparticles (Fe-CAP NPs) found it could inhibit secretion inflammatory cytokines alleviate Here, further explore anti-inflammatory mechanism Fe-CAP NPs. Methods Bone marrow-derived (BMDM) C57 mice were divided into four groups: control group, lipopolysaccharide (LPS) CAP + LPS group group. Western blot Immunofluorescence used detect expression macrophage phenotypic markers CD86 CD206 BMDM tissue. Fluorescence microbeads, Transwell ROS kit phagocytosis, migration clearing capability BMDM. was JAK2/STAT3 pathway apoptosis proteins TUNEL H&E staining evaluate pathological changes Results vitro , increased LPS, but decreased after pretreatment. opposite. reduced scavenged LPS-treated inhibited P-JAK2 P-STAT3 apoptosis. vivo improved histopathology tissue preconditioning, reversed. Conclusion NPs sepsis-induced ALI by regulating polarization function macrophages, reducing level

Язык: Английский

Emerging application of nanomedicine-based therapy in acute respiratory distress syndrome DOI

Yitianhe Xu,

Leyao Lv,

Qian Wang

и другие.

Colloids and Surfaces B Biointerfaces, Год журнала: 2024, Номер 237, С. 113869 - 113869

Опубликована: Март 21, 2024

Язык: Английский

Процитировано

10
Natural Compounds Regulate Macrophage Polarization and Alleviate Inflammation Against ALI/ARDS DOI Creative Commons
Zhinan Yin, Ruolin Song, Tong Yu

и другие.

Biomolecules, Год журнала: 2025, Номер 15(2), С. 192 - 192

Опубликована: Янв. 29, 2025

Acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) is a pulmonary disease with high mortality associated inflammation. During the development of ALI/ARDS, macrophages usually polarize toward M1 pro-inflammatory macrophages, promoting inflammatory response in ALI/ARDS and aggravating tissue damage. Natural compounds anti-inflammatory activity have achieved excellent results treatment through different regulatory modes, including macrophage polarization. Of note, flavonoid, brevilin A, tetrahydropalmatine play an important role by modulating phenotypic polarization their cytokine expression innate immune cells lung. Flavonoids are kind naturally occurring polyphenol compound, which has antioxidant activities. Studies found that some flavonoids can alleviate inhibiting cytokines macrophages. Among them, 5-methoxyflavone, acacetin, grape seed proanthocyanidins, luteolin also regulate Therefore, in-depth exploration mechanism lay foundation for application alleviating inflammation-related injury. This review focuses on effects natural potential mechanisms ALI/ARDS.

Язык: Английский

Процитировано

0
Therapeutic Effects of Nicotinic Acid from Bubali Cornu on H1n1-Induced Acute Lung Injury in Mice by Suppressing Nos2 Expression DOI

Guoqi Yuan,

Yang Liu,

Hong Zhou

и другие.

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
Adaptive bilirubin nanoscavenger alleviates pulmonary oxidative stress and inflammation for acute lung injury therapy DOI Creative Commons
Longfa Kou,

Yitianhe Xu,

Shize Li

и другие.

Journal of Advanced Research, Год журнала: 2025, Номер unknown

Опубликована: Март 1, 2025

Acute lung injury (ALI) is a life-threatening condition characterized by rapidly progressing respiratory distress and hypoxemia. Oxidative stress-induced inflammation in tissue plays crucial role the progression of ALI. Excessive generation reactive oxygen species (ROS) pulmonary microenvironment activates inflammatory signaling pathways, enhancing transcription pro-inflammatory factors ultimately leading to necrosis. Bilirubin (BR), an exceptional endogenous antioxidant, possesses ability counteract elevated levels through direct reactions or inducing antioxidant systems such as Nrf2/HO-1 signaling. However, its limited solubility poses hindrance further applications. Hence, it imperative develop suitable bilirubin-based system for biological utilization. In this study, we developed ROS-sensitive adaptive nanoscavenger (GP@BR) co-assembling bilirubin-conjugated glycol chitosan (GC-BR) polyethylene (PEG-BR), aiming alleviate oxidative stress ALI treatment. The different conjugations endowed bilirubin derivatives with varying sensitivity towards reacting ROS, enabling GP@BR exert antioxidative properties specifically environments on demand. Besides excellent properties, also demonstrated absorb excess cytokines. Moreover, our optimized facilitated transport across mucosal layer epithelial cells. vivo studies confirmed that significantly improved symptoms suppressed fibrosis. This study highlighted potential multiple actions treatment

Язык: Английский

Процитировано

0
Cordyceps Sinensis Reduces Inflammation and Protects BEAS-2B Cells From LPS-Induced THP-1 Cell Injury DOI Creative Commons
Xiaqing Wu, Xin Li, Ying Chai

и другие.

Journal of Inflammation Research, Год журнала: 2025, Номер Volume 18, С. 4143 - 4156

Опубликована: Март 1, 2025

Cordyceps sinensis, an entomogenous fungus with unique biological properties, has demonstrated significant anti-inflammatory potential. However, its effects on inflammation regulation need to be further investigated in detail. In this study, we aimed analyze the sinensis extract (CSE) obtained via ethanol extraction and assess regulation. The secretion of pro-inflammatory cytokines (IL-6, TNF-α, IL-8, IL-1β) level MMP9, Nrf2/HO-1 ROS were evaluated. A transwell system THP-1 BEAS-2B cells was used explore inflammatory damage. Gene ontology (GO) Kyoto encyclopedia genes genomes (KEGG) enrichment analyses conducted differentially expressed genes. CSE exhibited no cytotoxicity at concentrations ≤ 1.6 mg/mL. Treatment LPS-induced significantly inhibited cytokines. reduced inflammation-related protein while upregulating signaling pathway. Fluorescence assays using DCF JC-1 confirmed that help mitigate oxidative stress-induced inflammation. treatment protected from Moreover, immune process a shared GO term between LPS-only combined LPS treatment. KEGG analysis showed is capable regulating associated responses. These findings highlight potential as immune-regulating agent functional foods health products.

Язык: Английский

Процитировано

0
The involvement of Sting in exacerbating acute lung injury in sepsis via the PARP-1/NLRP3 signaling pathway DOI

Tingting Ying,

Yulong Yu,

Qimin Yu

и другие.

Pulmonary Pharmacology & Therapeutics, Год журнала: 2024, Номер 86, С. 102303 - 102303

Опубликована: Июнь 5, 2024

Язык: Английский

Процитировано

2
Human placental mesenchymal stem cells transplantation repairs the alveolar epithelial barrier to alleviate lipopolysaccharides-induced acute lung injury DOI Creative Commons

Wenqin Yu,

Yuzhen Lv,

Ruirui Xuan

и другие.

Biochemical Pharmacology, Год журнала: 2024, Номер 229, С. 116547 - 116547

Опубликована: Сен. 19, 2024

Язык: Английский

Процитировано

2
Hyperbaric oxygen-induced acute lung injury: A mouse model study on pathogenic characteristics and recovery dynamics DOI Creative Commons
Shu Wang, Hong Chen, Zhi Li

и другие.

Frontiers in Physiology, Год журнала: 2024, Номер 15

Опубликована: Окт. 18, 2024

Oxygen is an essential substance for the maintenance of human life. It also widely used in clinical and diving medicine. Although oxygen crucial survival, too much can be harmful. Excessive inhalation a short period time lead to injury, lung one main target organs. Acute injury (ALI) induced by hyperbaric (HBO) notably more severe than that caused normobaric oxygen, yet systematic research on such its regression scarce. In this study, two independent experiments were designed. first experiment, mice exposed 2 atmospheres absolute (ATA), ≥95% 2, 4, 6, 8 h. Changes histopathology, inflammation expression chemokines, alveolar-capillary barrier, 8-OHdG detected before after exposure. second these parameters measured at 0 h, 12 24 h following 6 exposure ATA oxygen. Research indicates ALI HBO characterized histologically alveolar expansion, atelectasis, inflammatory cell infiltration, hemorrhage. At ATA, significant changes barrier observed 95% 4 as evidenced increased Evans blue (EB) extravasation ( p = 0.0200). After exposure, tissue pathology scores, levels, chemotactic factors (such Il6, CCL2, CCL3, CXCL5, CXCL10), intercellular adhesion molecule 1 (ICAM1), vascular (VCAM1) significantly elevated. Compared onset was shortened. Additionally, it markers continued increase leaving environment, peaking starting recover indicating peak occurs within post-exposure, with recovery beginning This contradicts common belief alleviated upon removal from high-oxygen environment. However, EB which reflect damage VE-Cadherin (VE-Cad), tight junction protein (ZO-1), ICAM1, VCAM1 remained altered suggests most sensitive slowest recovering part HBO. These findings provide insights into pathogenesis progression offer references identifying corresponding intervention targets.

Язык: Английский

Процитировано

2
ROS responsive nanozyme loaded with STING silencing for the treatment of sepsis-induced acute lung injury DOI

Y. F. Zhang,

Lingyang Chen, Lin Feng

и другие.

Toxicology and Applied Pharmacology, Год журнала: 2024, Номер 493, С. 117155 - 117155

Опубликована: Ноя. 12, 2024

Язык: Английский

Процитировано

1
NAC-Grafted ROS-Scavenging Polymer Nanoparticles for Modulation of Acute Lung Injury Microenvironment In Vivo DOI
Wali Muhammad, Min Liang,

Beiduo Wang

и другие.

Biomacromolecules, Год журнала: 2024, Номер 26(1), С. 528 - 540

Опубликована: Дек. 27, 2024

N-Acetyl cysteine (NAC) is an essential molecule that boosts acute lung injury (ALI) defense via its direct antioxidant capability. Nevertheless, the therapeutic use of NAC limited due to poor bioavailability and short half-life. In this study, was grafted polyurethane consisting poly(propylene fumarate), poly(thioketal), 1,6-hexamethylene diisocyanate (PFTU) reduce excessive oxidative stress inflammatory factors in ALI. The NAC-grafted polymer nanoparticles (NPT@NPs) were prepared as a drug delivery system, which could effectively scavenge free radicals inflammation vitro. administration NPT@NPs exhibited notable efficacy ameliorating pulmonary edema, attenuating presence cells, suppressing myeloperoxidase expression, diminishing levels pro-inflammatory cytokines, reversing cell apoptosis ALI model induced by lipopolysaccharide (LPS). demonstrated significantly better compared mitigating deleterious effects LPS on tissue, thereby providing more effective protection against inflammation.

Язык: Английский

Процитировано

1