Engineered mitochondrial ROS scavenger nanocomplex to enhance lung biodistribution and reduce inflammation for the treatment of ARDS

Advanced Composites and Hybrid Materials, Год журнала: 2024, Номер 7(6)

Опубликована: Окт. 23, 2024

Язык: Английский

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Magnesium hydride attenuates intestinal barrier injury during hemorrhage shock by regulating neutrophil extracellular trap formation via the ROS/MAPK/PAD4 pathway

International Immunopharmacology, Год журнала: 2024, Номер 130, С. 111688 - 111688

Опубликована: Фев. 22, 2024

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Ribociclib leverages phosphodiesterase 4 inhibition in the treatment of neutrophilic inflammation and acute respiratory distress syndrome

Journal of Advanced Research, Год журнала: 2024, Номер 62, С. 229 - 243

Опубликована: Март 27, 2024

Overwhelming neutrophil activation and oxidative stress significantly contribute to acute respiratory distress syndrome (ARDS) pathogenesis. However, the potential of repurposing ribociclib, a cyclin-dependent kinase 4 6 (CDK4/6) inhibitor used clinically in cancer treatment, for treating neutrophilic ARDS remains uncertain. This study illustrated ability underlying mechanism ribociclib inflammation.

Язык: Английский

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CTSO and HLA-DQA1 as biomarkers in sepsis-associated ARDS: insights from RNA sequencing and immune infiltration analysis

BMC Infectious Diseases, Год журнала: 2025, Номер 25(1)

Опубликована: Март 7, 2025

The onset of sepsis frequently coincides with acute respiratory distress syndrome (ARDS), which constitutes a significant contributor to severe acid–base disturbances in septic patients. In the pathogenesis sepsis, it conducts crucial role. lysosomal metabolic disorders and immune imbalance conduct pivotal Despite extensive research into alterations status during few studies have been reported thoroughly examine association between lysosomes sepsis. As result, this study is predominantly Intended delve link lysosome-related genes lysosome microenvironment from standpoint bioinformatics Registration Number was ChiCTR1900021261. Date 2019/02/04. Method Sepsis data source: collected previous clinical sequencing results (Originated BGI Shenzhen Co., Ltd.) GO database utilized for collection genes. Differential expression (DEGs) were screened on by employing IDEP 0.93 software subsequent quality control. Afterwards, enrichment analysis conducted adopting Gene Set Enrichment Analysis (GSEA) Weighted Co Network (WGCNA), followed cross referencing identify DEGs associated lysosomes. KEGG pathway wereperformed subsequently. obtained PLSGs WGCNA Creating PPI network entails following steps: points intersected at first. CytoHubba MCODE performed utilizing cytoscape software. Next, intersection taken confirm Hub gene sequences, subsequently central tightly existing CTD scores. Notwithstanding fact that causes are multifaceted, ARDS can often trigger development numerous cases. Simultaneously, an aim predict transcription factor levels nervous system, Cytoscape adopted find relevant target miRNAs miRWalk database, correlated regulatory established accordingly. SEPSIS infiltration model constructed ImmuCellAI abundance Spearman's method. Last but not least, worth noting single-cell has validated as method analyze hub cells patients, enabling selection key closely predictive outcomes. Result When (ARDS) present, differentially expressed implicated metabolism regulation microenvironment. Six bound up or attributable examinations. On top that, determined patients had syndrome. illustrated remarkable augment T cell while relative DC reduced certain level. Positive correlations found two Spearman role cells. Moreover, universally acknowledged anti-inflammatory responsible negative correlation. basis sequencing, CTSO HLA-DQA1 Aside survival-death curve direction suggested they could be core predicting sepsis-related prognosis analysis. Conclusion An demonstrates interaction changes understanding basis, we develop new diagnostics therapeutic approaches identifying drug targets. Nonetheless, differ simply difference site infection; etiology cases quite complex, progression occur if infection exacerbates other complications arise, meeting diagnostic criteria 3.0.

Язык: Английский

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Scutellaria barbata ameliorates acute respiratory distress syndrome by inhibiting neutrophil-mediated inflammatory responses

Journal of Ethnopharmacology, Год журнала: 2025, Номер unknown, С. 119653 - 119653

Опубликована: Март 1, 2025

Язык: Английский

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Ferulic Acid Alleviates Mycoplasma pneumoniae -induced Pneumonia in Mice by Regulation of Inflammation and ERK/JNK/NF-κB Pathway

Pharmacognosy Magazine, Год журнала: 2025, Номер unknown

Опубликована: Апрель 14, 2025

Background Pneumonia is a serious respiratory illness that impacts the lungs, leading to inflammation and fluid accumulation in alveoli, tiny air sacs essential for gas exchange. Mycoplasma pneumonia severe infection caused by atypical bacterium pneumoniae (MP). Purpose The primary objective of this work explore therapeutic effects ferulic acid against MP-infected an experimental mice model. Methods In work, BALB/c were subjected 100 µL MP challenge via nasal drips trigger pneumonia. then administered 50 mg/kg 3 days. After experimentation, lung weight, myeloperoxidase (MPO), nitric oxide (NO) concentrations assessed. inflammatory cytokines oxidative stress biomarkers analyzed using appropriate diagnostic kits. total cell count bronchoalveolar lavage (BAL) deoxyribonucleic (DNA) concentration lungs assessed mice. histopathology examination was conducted evaluate histological abnormalities. Results administration significantly decreased MPO, NO Ferulic treatment resulted diminution malondialdehyde (MDA) enhancement glutathione (GSH) superoxide dismutase (SOD) concentrations. diminished marker MP-challenged Furthermore, reduced DNA content counts addition, outcomes indicated significant reduction alveolar damage with Conclusion findings confirm salutary properties Consequently, may serve as effective drug candidate treatment.

Язык: Английский

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Targeting Glutamate Transport: A Breakthrough in Mitigating Sepsis Lung Injury

Free Radical Biology and Medicine, Год журнала: 2025, Номер 235, С. 190 - 199

Опубликована: Апрель 26, 2025

Язык: Английский

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Suppression of NOX2-Derived Reactive Oxygen Species (ROS) Reduces Epithelial-to-MesEnchymal Transition Through Blocking SiO2-Regulated JNK Activation

Toxics, Год журнала: 2025, Номер 13(5), С. 365 - 365

Опубликована: Апрель 30, 2025

(1) Background: Silicosis, a chronic lung fibrosis disorder triggered by the accumulation of silica dust in deep regions, is characterized intricate molecular mechanisms. Among these, NOX2 (NADPH oxidase 2) and JNK (C-Jun N-terminal kinase) signaling pathways play pivotal roles progression pulmonary fibrosis. Despite their significance, precise mechanisms underlying crosstalk between these remain largely unexplored. (2) Methods: To unravel interactions, we examined interplay human epithelial cells subjected to exposure through vivo assays, followed validation using single-cell sequencing. Our findings consistently revealed elevated expression levels key components from both pathway lungs silicosis-induced mice silica-treated cells. (3) Results: Notably, activation was linked increased ROS (reactive oxygen species) production, profibrogenic factors, diminished cell proliferation silica-exposed Further mechanistic analyses demonstrated that amplifies production induced exposure, while treatment with inhibitor SP600125 mitigates effects. Conversely, overexpression enhanced silica-induced epithelial–mesenchymal transition (EMT)-related whereas knockdown exerted opposite effect. These results suggest positive feedback loop signaling, which may drive EMT following exposure. (4) Conclusions: This reciprocal interaction appears critical role damage pathogenesis silicosis, shedding light on disease offering potential avenues for therapeutic intervention.

Язык: Английский

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siRNA-Mediated Timp1 Silencing Inhibited the Inflammatory Phenotype during Acute Lung Injury

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(2), С. 1641 - 1641

Опубликована: Янв. 13, 2023

Acute lung injury is a complex cascade process that develops in response to various damaging factors, which can lead acute respiratory distress syndrome. Within this study, based on bioinformatics reanalysis of available full-transcriptome data induced mice and humans by we selected set genes could serve as good targets for suppressing inflammation the tissue, evaluated their expression cells different origins during LPS-induced inflammation, chose tissue inhibitor metalloproteinase Timp1 promising target inflammation. We designed an effective chemically modified anti-TIMP1 siRNA showed silencing correlates with decrease pro-inflammatory cytokine IL6 secretion cultured macrophage reduces severity mouse model.

Язык: Английский

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Drug repurposing of cyclin-dependent kinase inhibitors for neutrophilic acute respiratory distress syndrome and psoriasis

Journal of Advanced Research, Год журнала: 2024, Номер unknown

Опубликована: Июль 1, 2024

Neutrophilic inflammation, characterized by dysregulated neutrophil activation, triggers a variety of inflammatory responses such as chemotactic infiltration, oxidative bursts, degranulation, extracellular traps (NETs) formation, and delayed turnover. This type inflammation is pivotal in the pathogenesis acute respiratory distress syndrome (ARDS) psoriasis. Despite current treatments, managing neutrophil-associated symptoms remains significant challenge. review emphasizes role cyclin-dependent kinases (CDKs) activation inflammation. It aims to highlight therapeutic potential repurposing CDK inhibitors manage neutrophilic particularly ARDS Additionally, it discusses necessary precautions for clinical application these due off-target effects need dose optimization. CDKs regulate key functions, including responses, NET apoptosis. Repurposing inhibitors, originally developed cancer treatment, shows promise controlling Clinical anticancer drugs, palbociclib ribociclib, have demonstrated efficacy treating psoriasis targeting off-label pathways, phosphoinositide 3-kinase (PI3K) phosphodiesterase 4 (PDE4), respectively. While offer promising benefits, their requires careful consideration Further exploration trials are ensure safety conditions.

Язык: Английский

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