The Lachnospiraceae-butyric acid axis and its role in glucocorticoid-associated osteonecrosis DOI Creative Commons

Mingbin Guo,

Shuai He, Wei Song

и другие.

Journal of Translational Medicine, Год журнала: 2024, Номер 22(1)

Опубликована: Ноя. 11, 2024

Glucocorticoids (GCs) are key inducers of osteonecrosis, yet not all patients treated with GCs develop glucocorticoid-associated osteonecrosis (GAON). The factors mediating this relationship unclear. Studies have shown that gut microbiota and their metabolites influence bone metabolism, but role in GAON is This study aimed to explore the connection between microbiota. Through bidirectional Mendelian randomization analysis, we identified 14 microbial taxa, including Lachnospiraceae (IVW, P = 0.011), associated GAON. RNA-seq analysis revealed differentially expressed genes (DEGs) were enriched for intestinal inflammatory response mechanisms. We then compared who developed (17 cases), those did (GAnON, 15 untreated (Blank, cases) composition, short-chain fatty acids (SCFAs), serum factors. Our findings indicated a decrease abundance (GAON 17.13%, GAnON 12.51%, Blank 24.52%) GC-treated patients. Serum (IL-17 A, IL-33, TNF-α) (59.603 ± 12.147, 89.337 20.714, 42.584 9.185) showed significant differences (1.446 0.683, 11.534 4.705, 4.682 1.48) (25.353 8.181, 32.527 7.352, 12.49 3.217) groups, negative correlation these levels. Butyric acid levels SCFAs varied among groups (P<0.01) correlated Controlled experiments rats demonstrated butyric acid's osteoprotective development (P<0.01). In conclusion, our suggests reduced levels, along increased inflammation due use, contribute may mediate effects inflammation. Butyrate supplementation could potentially reduce incidence, offering novel approach its clinical management.

Язык: Английский

The Lachnospiraceae-butyric acid axis and its role in glucocorticoid-associated osteonecrosis DOI Creative Commons

Mingbin Guo,

Shuai He, Wei Song

и другие.

Journal of Translational Medicine, Год журнала: 2024, Номер 22(1)

Опубликована: Ноя. 11, 2024

Glucocorticoids (GCs) are key inducers of osteonecrosis, yet not all patients treated with GCs develop glucocorticoid-associated osteonecrosis (GAON). The factors mediating this relationship unclear. Studies have shown that gut microbiota and their metabolites influence bone metabolism, but role in GAON is This study aimed to explore the connection between microbiota. Through bidirectional Mendelian randomization analysis, we identified 14 microbial taxa, including Lachnospiraceae (IVW, P = 0.011), associated GAON. RNA-seq analysis revealed differentially expressed genes (DEGs) were enriched for intestinal inflammatory response mechanisms. We then compared who developed (17 cases), those did (GAnON, 15 untreated (Blank, cases) composition, short-chain fatty acids (SCFAs), serum factors. Our findings indicated a decrease abundance (GAON 17.13%, GAnON 12.51%, Blank 24.52%) GC-treated patients. Serum (IL-17 A, IL-33, TNF-α) (59.603 ± 12.147, 89.337 20.714, 42.584 9.185) showed significant differences (1.446 0.683, 11.534 4.705, 4.682 1.48) (25.353 8.181, 32.527 7.352, 12.49 3.217) groups, negative correlation these levels. Butyric acid levels SCFAs varied among groups (P<0.01) correlated Controlled experiments rats demonstrated butyric acid's osteoprotective development (P<0.01). In conclusion, our suggests reduced levels, along increased inflammation due use, contribute may mediate effects inflammation. Butyrate supplementation could potentially reduce incidence, offering novel approach its clinical management.

Язык: Английский

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