Association of inflammation and protein carbamylation in patients with COVID-19
Frontiers in Medicine,
Год журнала:
2025,
Номер
12
Опубликована: Апрель 2, 2025
Carbamylation
involves
the
non-enzymatic
binding
of
isocyanic
acid
to
amino
groups
proteins,
making
it
associated
with
many
pathological
conditions,
including
inflammation,
aging,
arteriosclerosis,
and
renal
failure.
However,
there
are
no
data
on
protein
carbamylation
in
patients
COVID-19.
Our
study
is
first
evaluate
association
between
blood
inflammation
who
died
from
COVID-19
compared
survivors.
The
included
50
admitted
Dr.
Tytus
Chałubiński
Specialist
Hospital
Radom,
Poland.
Twenty-five
them
were
survivors
(15
men,
10
women),
25
deceased
women).
number
subjects
was
based
a
pilot
assuming
significance
level
0.05
test
power
0.8.
Plasma/serum
samples
assayed
for
carbamyl-lysine
(CBL)
inflammatory
biomarkers
(CRP,
procalcitonin,
D-dimer,
IL-6,
WBC).
concentration
CBL
measured
using
an
enzyme-linked
immunosorbent
assay
(ELISA).
Statistical
analysis
performed
Mann-Whitney
U
Spearman
rank
correlation.
Receiver
Operating
Characteristic
(ROC)
used
assess
diagnostic
utility
serum
CBL.
Serum
levels
significantly
higher
(p
=
0.0011).
There
positive
correlation
WBC.
>101
ng/mL,
moderate
sensitivity
specificity,
differentiate
recovered
(area
under
curve
0.76).
In
conclusion,
excessive
carbamylation.
Inflammation
may
be
source
production
A
thorough
understanding
consequences
increased
clarify
complications.
Язык: Английский
Oxidative stress indexes as biomarkers of the severity in COVID-19 patients
International Journal of Medical Sciences,
Год журнала:
2024,
Номер
21(15), С. 3034 - 3045
Опубликована: Ноя. 11, 2024
Background:
SARS-CoV-2
causes
a
global
pandemic,
with
severe
and
critically
ill
COVID-19
patients
often
experiencing
poor
prognoses.
Severe
infection
is
associated
oxidative
stress
(OS)
inflammation.
Detecting
markers
of
macromolecular
damage
caused
by
OS
may
provide
valuable
insights
into
disease
progression.
Methods:
This
study
included
187
laboratory-confirmed
infection,
categorized
non-severe,
severe,
groups.
We
monitored
the
changes
in
serum
indexes
such
as
oxidized
low-density
lipoprotein
(OxLDL),
OxLDL/LDL-C
ratio,
advanced
oxidation
protein
products
(AOPP),
3-nitrotyrosine
(3-NT),
8-hydroxydeoxyguanosine
(8-OHdG),
lipoprotein-associated
phospholipase
A2
(Lp-PLA2)
thromboxane
B2
(TXB2)
different
clinical
types.
Results:
48
non-severe
patients,
90
49
were
enrolled.
Compared
group,
OxLDL
level
ratio
increased
while
3-NT
TXB2
concentrations
lower
patients.
Critically
also
exhibited
Lp-PLA2
higher
compared
to
No
significant
differences
observed
AOPP
8-OHdG
concentrations.
Spearman's
correlation
analysis
revealed
that
CRP
was
OxLDL,
AOPP,
3-NT,
TXB2,
(P
<0.05).
identified
an
independent
risk
factor
for
progression
from
severe/critically
COVID-19.
demonstrated
good
discriminatory
value
between
COVID-19,
distinguishing
Conclusion:
Patients
exhibit
elevated
levels
lipoproteins.
can
serve
biomarkers
assessing
severity
Язык: Английский
Total Antioxidant and Oxidative Status as Potential Biomarkers of Alcohol Overdose
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
26(1), С. 82 - 82
Опубликована: Дек. 25, 2024
Serious
alcohol-associated
hazards
underscore
the
need
to
develop
new
biomarkers
reflecting
biological
changes
caused
by
chronic
alcohol
use
and
predicting
risk
of
alcohol-related
death.
Oxidative
stress
is
one
mechanism
toxicity.
The
blood
urine
redox
status
(total
antioxidant
capacity
[TAC],
total
oxidative
[TOS],
index
[OSI])
was
assessed
in
105
people
who
died
a
sudden
death
(controls),
47
overdose,
102
with
dependency.
TAC
TOS
were
determined
utilizing
colorimetric
method.
Non-parametric
tests
used
for
statistical
analysis.
Blood
levels
significantly
elevated
individuals
both
dependency
poisoning
compared
controls.
study
groups
control
group,
higher
patients
group
poisoning.
highly
correlated
content.
Receiver
operating
characteristic
(ROC)
analysis
showed
that
effectively
discriminated
between
high
sensitivity
specificity.
Our
confirmed
impaired
homeostasis
alcoholism
indicated
utility
TAC,
TOS,
OSI
as
exposure.
Язык: Английский