Identification and validation of diagnostic genes IFI44 and IRF9 in insomnia-associated autoimmune uveitis

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Янв. 31, 2025

Objective Insomnia is increasingly recognized as a significant factor in the development of various autoimmune diseases, including uveitis (AU). We investigated insomnia-associated genes that may contribute to AU pathogenesis and sought identify potential biomarkers for AU. Methods Microarray data related insomnia were downloaded from Gene Expression Omnibus (GEO) database analyzed. The GEO2R tool was used differentially expressed (DEGs) common between Weighted gene co-expression network analysis (WGCNA), protein-protein interaction (PPI), functional enrichment, CMap analyses then performed pathogenic genes, underlying mechanisms, therapeutic drugs Least absolute shrinkage selection operator regression employed screen candidate biomarkers, their diagnostic performance evaluated using receiver operating characteristic (ROC) curves quantitative polymerase chain reaction (qPCR). Finally, molecular docking applied verify binding activities. Results identified 138 up-regulated 85 down-regulated DEGs PPI highlighted 10 key 30 compounds, WGCNA revealed 54 compounds. Intersection above-mentioned compounds six five After verification by qPCR ROC curve analysis, IFI44 IRF9 confirmed hub genes. two selected based on scores less than −7 kcal/mol. Conclusion Our study demonstrated involvement viral response both significance these conditions. These findings provide novel insights future strategies treat

Язык: Английский

Tumor-associated antigens are associated with primary Sjögren’s syndrome-related interstitial lung disease and disease activity

Clinical Biochemistry, Год журнала: 2025, Номер unknown, С. 110927 - 110927

Опубликована: Апрель 1, 2025

Язык: Английский