Identification of biomarkers and potential drug targets for esophageal cancer: a Mendelian randomization study
Scientific Reports,
Год журнала:
2025,
Номер
15(1)
Опубликована: Март 10, 2025
Язык: Английский
From Classical to Alternative Pathways of 2-Arachidonoylglycerol Synthesis: AlterAGs at the Crossroad of Endocannabinoid and Lysophospholipid Signaling
Molecules,
Год журнала:
2024,
Номер
29(15), С. 3694 - 3694
Опубликована: Авг. 4, 2024
2-arachidonoylglycerol
(2-AG)
is
the
most
abundant
endocannabinoid
(EC),
acting
as
a
full
agonist
at
both
CB1
and
CB2
cannabinoid
receptors.
It
synthesized
on
demand
in
postsynaptic
membranes
through
sequential
action
of
phosphoinositide-specific
phospholipase
Cβ1
(PLCβ1)
diacylglycerol
lipase
α
(DAGLα),
contributing
to
retrograde
signaling
upon
interaction
with
presynaptic
CB1.
However,
2-AG
production
might
also
involve
various
combinations
PLC
DAGL
isoforms,
well
additional
intracellular
pathways
implying
other
enzymes
substrates.
Three
alternative
synthesis
rest
extracellular
cleavage
2-arachidonoyl-lysophospholipids
by
three
different
hydrolases:
glycerophosphodiesterase
3
(GDE3),
lipid
phosphate
phosphatases
(LPPs),
two
members
ecto-nucleotide
pyrophosphatase/phosphodiesterases
(ENPP6-7).
We
propose
names
AlterAG-1,
-2,
-3
for
sharing
an
ectocellular
localization,
allowing
them
convert
lysophospholipid
mediators
into
2-AG,
thus
inducing
typical
switches
between
G-protein-coupled
receptors
(GPCRs).
This
implies
critical
importance
regioisomerism
(LPLs)
which
object
deep
analysis
within
this
review.
The
precise
functional
roles
AlterAGs
are
still
poorly
understood
will
require
gene
invalidation
approaches,
knowing
that
its
related
lysophospholipids
involved
numerous
aspects
physiology
pathology,
including
cancer,
inflammation,
immune
defenses,
obesity,
bone
development,
neurodegeneration,
or
psychiatric
disorders.
Язык: Английский
Bioinformatic analysis and experimental validation of six cuproptosis-associated genes as a prognostic signature of breast cancer
PeerJ,
Год журнала:
2024,
Номер
12, С. e17419 - e17419
Опубликована: Июнь 18, 2024
Breast
carcinoma
(BRCA)
is
a
life-threatening
malignancy
in
women
and
shows
poor
prognosis.
Cuproptosis
novel
mode
of
cell
death
but
its
relationship
with
BRCA
unclear.
This
study
attempted
to
develop
cuproptosis-relevant
prognostic
gene
signature
for
BRCA.
Cuproptosis-relevant
subtypes
were
obtained
by
consensus
clustering.
Differential
expression
analysis
was
implemented
using
the
'limma'
package.
Univariate
Cox
multivariate
analyses
performed
determine
signature.
The
constructed
validated
distinct
datasets.
Gene
set
variation
(GSVA)
enrichment
(GSEA)
also
conducted
uncover
underlying
molecular
mechanisms.
ESTIMATE
CIBERSORT
algorithms
applied
probe
linkage
between
tumor
microenvironment
(TME).
Immunotherapy
responsiveness
assessed
Tumor
Immune
Dysfunction
Exclusion
(TIDE)
web
tool.
Real-time
quantitative
PCR
(RT-qPCR)
detect
expressions
genes
breast
cancer
lines.
Thirty-eight
cuproptosis-associated
differentially
expressed
(DEGs)
mined
clustering
differential
analysis.
Based
on
univariate
analyses,
six
genes,
namely
SAA1,
KRT17,
VAV3,
IGHG1,
TFF1,
CLEC3A,
establish
corresponding
external
validation
sets.
GSVA
GSEA
showed
that
multiple
cycle-linked
immune-related
pathways
along
biological
processes
associated
results
revealed
significantly
different
TMEs
two
Cusig
score
subgroups.
Finally,
RT-qPCR
lines
further
confirmed
expressional
trends
CLEC3A.
Taken
together,
we
projecting
overall
survival
patients
our
findings
authenticated
which
are
expected
provide
basis
developing
biomarkers
an
in-depth
understanding
cuproptosis
Язык: Английский