Advanced Glycation End-Product-Modified Heat Shock Protein 90 May Be Associated with Urinary Stones
Diseases,
Год журнала:
2025,
Номер
13(1), С. 7 - 7
Опубликована: Янв. 2, 2025
Urinary
stones
(urolithiasis)
have
been
categorized
as
kidney
(renal
calculus),
ureteric
(ureteral
calculus
and
ureterolith),
bladder
(bladder
urethral
(urethral
calculus);
however,
the
mechanisms
underlying
their
promotion
related
injuries
in
glomerular
tubular
cells
remain
unclear.
Although
lifestyle-related
diseases
(LSRDs)
such
hyperglycemia,
type
2
diabetic
mellitus,
non-alcoholic
fatty
liver
disease/non-alcoholic
steatohepatitis,
cardiovascular
disease
are
risk
factors
for
urolithiasis,
Recently,
heat
shock
protein
90
(HSP90)
on
membrane
of
HK-2
human
proximal
epithelium
has
associated
with
adhesion
urinary
cytotoxicity.
Further,
HSP90
pancreatic
breast
can
be
modified
by
various
advanced
glycation
end-products
(AGEs),
thus
affecting
function.
Hypothesis
1:
We
hypothesized
that
HSP90s
on/in
types
AGEs,
they
may
affect
functions
it
a
key
to
reveal
LSRDs
urolithiasis.
2:
considered
possibility
Japanese
traditional
medicines
urolithiasis
inhibit
AGE
generation.
Of
Choreito
Urocalun
(the
extract
Quercus
salicina
Blume/Quercus
stenophylla
Makino)
used
clinic,
is
Kampo
medicine,
while
characteristic
medicine.
As
contains
quercetin,
hesperidin,
p-hydroxy
cinnamic
acid,
which
generation,
we
generation
AGE-modified
cells.
Язык: Английский
Slot Blot- and Electrospray Ionization–Mass Spectrometry/Matrix-Assisted Laser Desorption/Ionization–Mass Spectrometry-Based Novel Analysis Methods for the Identification and Quantification of Advanced Glycation End-Products in the Urine
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(17), С. 9632 - 9632
Опубликована: Сен. 5, 2024
Proteins,
saccharides,
and
low
molecular
organic
compounds
in
the
blood,
urine,
saliva
could
potentially
serve
as
biomarkers
for
diseases
related
to
diet,
lifestyle,
use
of
illegal
drugs.
Lifestyle-related
(LSRDs)
such
diabetes
mellitus
(DM),
non-alcoholic
steatohepatitis,
cardiovascular
disease,
hypertension,
kidney
osteoporosis
develop
into
life-threatening
conditions.
Therefore,
there
is
an
urgent
need
their
early
diagnosis.
Advanced
glycation
end-products
(AGEs)
are
associated
with
LSRDs
may
induce/promote
LSRDs.
The
presence
AGEs
body
fluids
represent
a
biomarker
Urine
samples
be
used
detecting
AGEs,
urine
collection
convenient
non-invasive.
However,
detection
identification
AGE-modified
proteins
challenging,
concentrations
might
extremely
low.
To
address
this
issue,
we
propose
new
analytical
approach.
This
strategy
employs
method
previously
introduced
by
us,
which
combines
slot
blotting,
our
unique
lysis
buffer
named
Takata’s
buffer,
polyvinylidene
difluoride
membrane,
conjunction
electrospray
ionization-mass
spectrometry
(ESI)/matrix-assisted
laser
desorption/ionization-mass
(MALDI-MS).
novel
detect
proteins,
peptides,
free-type
samples.
Язык: Английский