Biochar supplementation affects the microbiome of recycled manure solids for cow bedding: a metagenomic analysis DOI Creative Commons
Joana F. Guerreiro, Ana José Pires, Mónica Nunes

и другие.

Journal of Dairy Science, Год журнала: 2024, Номер unknown

Опубликована: Дек. 1, 2024

The widespread use of Recycled Manure Solids (RMS) as cow bedding material is not without risks, since cattle manure may act a vehicle for pathogenic and antimicrobial resistant bacteria dissemination. Thus, our aim was to evaluate RMS-supplemented with pine biochar produced in Portugal new material, the has been shown have potential mitigate impact relevant bacterial species when added animal microbiota. Our experimental setup consisted on fresh RMS samples that were collected commercial dairy farm placed naturally-ventilated containers total 4 groups: 1-non-supplemented RMS; 2-RMS supplemented 2.5% (wt/wt) biochar; 3-RMS 5% 4-RMS 10% biochar. Sampling performed at different incubation times (0, 5, 15 30 d) 2 distinct seasons: April-May (humid season) June-July (dry season). resulting 32 subjected DNA extraction their microbiome profile determined through complete 16S rDNA gene sequencing using Nanopore next-generation sequencing. We observed supplementation clearly altered RMS, which reflected changes populations' diversity relative abundance bacteria. In particular, we found long-term storage (30 more beneficial than short-term storage, an effect evident or both seasons, those concentrations led decrease levels several mastitis-causing agents (Enterobacteriaceae, streptococci, enterococci staphylococci). addition, also reduction Salmonella spp. Gram-positive bacilli biochar-supplemented samples. Unexpectedly, however, same conditions yielded increase Brucella spp., group includes important infectious agents, highlighting need deeper evaluation ensure future safe sustainable this environmental-friendly resource production.

Язык: Английский

Potential of Pine Biochar to Mitigate Bacterial Hazards Present in Recycled Manure Solids from Dairy Cows DOI Creative Commons
Ana José Pires, Ana Rita Mendes Esteves, Gonçalo Pereira

и другие.

Veterinary Sciences, Год журнала: 2025, Номер 12(1), С. 43 - 43

Опубликована: Янв. 10, 2025

The use of recycled manure solids (RMS) as cow bedding in dairy farms poses concerns due to its potential harbor pathogenic and antimicrobial-resistant bacteria. This study evaluated the impact RMS supplementation with biochar at three concentrations (2.5%, 5%, 10%) on bacterial counts antimicrobial resistance virulence profiles Escherichia coli Enterococcus isolates. results show that did not promote a significant reduction numbers. Overall, there were no changes isolates’ or profiles, none isolates obtained classified high moderate threats based their MAR VIR indexes. most found present control samples. A phylogenetic analysis E. allowed us identify phylogroup D strains, predominantly supplemented 2.5% 10% biochar, which are associated higher resistance. These findings indicate tested effective significantly reducing risks RMS. Further research is needed evaluate different formulations concentrations, aiming optimize for supplementation.

Язык: Английский

Процитировано

1
Antimicrobial Resistance: The Impact from and on Society According to One Health Approach DOI Creative Commons
María Pía Ferraz

Societies, Год журнала: 2024, Номер 14(9), С. 187 - 187

Опубликована: Сен. 17, 2024

Antibiotics are drugs that target and destroy bacteria, they hailed as one of the most amazing medical breakthroughs 20th century. They have completely changed how we treat infections saved countless lives. But their usefulness is not limited to just medicine; also been used for many years in sectors like farming prevent animals, especially less wealthy countries. Antimicrobial resistance (AMR) ability microorganisms such viruses, fungi, parasites resist effects antimicrobial agents, antibiotics, antivirals, antifungals, antiparasitics, were once effective treating caused by these organisms. AMR presents an intricate challenge endangers health both humans well global economy, security nations world at large. Because resistant bacteria swiftly appearing spreading among humans, environment worldwide, acknowledged a within framework One Health. The Health approach involves cooperation various fields attain best possible outcomes environment. It acknowledges interconnectedness human, animal, environmental health. merely scientific or issue; it societal demands collective action awareness. In tapestry society, every thread contributes fabric AMR, individual holds stake its resolution.

Язык: Английский

Процитировано

5
Rational computational design and development of an immunogenic multiepitope vaccine incorporating transmembrane proteins of Fusobacterium necrophorum DOI Creative Commons
Muhammad Naveed,

Muhammad Toheed,

Tariq Aziz

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Май 4, 2025

Fusobacterium necrophorum is a Gram-negative, anaerobic pathogen responsible for Lemierre's syndrome, bovine foot rot, and other necrotizing infections. The rise in antimicrobial resistance the absence of effective vaccines underscore need alternative therapeutic strategies. This study employs computational biology to design multi-epitope vaccine targeting transmembrane proteins F. elicit strong immune responses. selected were evaluated toxicity, allergenicity, antigenicity, followed by epitope prediction screening. B T cell epitopes linked using immunogenic linkers, forming construct with VaxiJen score 0.7293 solubility 8.30 E. coli. Structural validation TrRosetta Ramachandran plots confirmed 97.4% residues favored regions, indicating high stability. Population coverage analysis indicated over 99% global applicability, further enhancing its potential impact. Docking studies revealed interactions receptors TLR7 TLR8. formed 12 hydrogen bonds, while TLR8(A) 9, TLR8(B) exhibited highest interaction, 13 bonds construct. Molecular dynamics simulations structural stability receptor engagement. RMSD stabilized around 4-5 Å, Vaccine-TLR8(B) complex. Radius Gyration remained 36 showing slight compaction time, RMSF peaked at 8-9 Å flexible lower fluctuations (1.5-2.5 Å) stable core regions. Principal component (PCA) identified elastic regions critical biological activity, energy levels (-5000 kJ/mol) reliability binding. Moreover, expression coli, as demonstrated SnapGene software pET-29a( +) vector. binding affinities predicted activation both humoral cellular responses, including increased IgM, IgG, cytokine levels. However, experimental necessary confirm safety efficacy, challenges manufacturing variable responses across populations must also be addressed.

Язык: Английский

Процитировано

0
Biochar supplementation affects the microbiome of recycled manure solids for cow bedding: a metagenomic analysis DOI Creative Commons
Joana F. Guerreiro, Ana José Pires, Mónica Nunes

и другие.

Journal of Dairy Science, Год журнала: 2024, Номер unknown

Опубликована: Дек. 1, 2024

The widespread use of Recycled Manure Solids (RMS) as cow bedding material is not without risks, since cattle manure may act a vehicle for pathogenic and antimicrobial resistant bacteria dissemination. Thus, our aim was to evaluate RMS-supplemented with pine biochar produced in Portugal new material, the has been shown have potential mitigate impact relevant bacterial species when added animal microbiota. Our experimental setup consisted on fresh RMS samples that were collected commercial dairy farm placed naturally-ventilated containers total 4 groups: 1-non-supplemented RMS; 2-RMS supplemented 2.5% (wt/wt) biochar; 3-RMS 5% 4-RMS 10% biochar. Sampling performed at different incubation times (0, 5, 15 30 d) 2 distinct seasons: April-May (humid season) June-July (dry season). resulting 32 subjected DNA extraction their microbiome profile determined through complete 16S rDNA gene sequencing using Nanopore next-generation sequencing. We observed supplementation clearly altered RMS, which reflected changes populations' diversity relative abundance bacteria. In particular, we found long-term storage (30 more beneficial than short-term storage, an effect evident or both seasons, those concentrations led decrease levels several mastitis-causing agents (Enterobacteriaceae, streptococci, enterococci staphylococci). addition, also reduction Salmonella spp. Gram-positive bacilli biochar-supplemented samples. Unexpectedly, however, same conditions yielded increase Brucella spp., group includes important infectious agents, highlighting need deeper evaluation ensure future safe sustainable this environmental-friendly resource production.

Язык: Английский

Процитировано

1