SAINSTEK International Journal on Applied Science Advanced Technology and Informatics,
Год журнала:
2022,
Номер
1(02), С. 39 - 45
Опубликована: Дек. 27, 2022
Viruses
are
obligate
intracellular
parasites
because
they
can
infect
cells
and
hijack
the
gene
expression
process
in
host
for
replication
of
viral
genetic
material.
The
mechanism
life
cycle
is
generally
divided
into
three
stages
such
as
entry,
genome
replication,
budding,
release.
Several
viruses
inhibit
interferon
signaling
through
JAK/STAT
pathway
human
cytomegalovirus
(HCMV),
HIV-1,
murine
hepatitis
virus
(MHV),
severe
acute
respiratory
syndrome
coronavirus
(SARS-CoV-2),
&
vaccinia
(VACV).
Antiviral
drugs
be
developed
by
studying
how
evade
immune
response,
several
have
been
discovered
but
most
them
synthetic
compounds
that
produce
use
effect.
Screening
drug
candidate
carried
out
on
a
specific
natural
ingredient
green
tea
used
this
study.
Scientific
evidence
from
an
antiviral
agent
very
little,
study
important
it
to
screen
potential
computational
approach.
Green
Tea
phytochemical
predicted
based
silico
analysis.
EGCG,
ECG,
EGC,
EC,
Catechin,
drug-like
molecules
positive
probabilities
agents.
Journal of Agriculture and Food Research,
Год журнала:
2024,
Номер
16, С. 101129 - 101129
Опубликована: Март 26, 2024
Chlorella
protein
hydrolysate
(CPH)
is
highly
valued
for
its
content,
solubility,
and
functionality.
Our
main
aim
to
produce
CPH
via
enzymatic
hydrolysis
using
pepsin,
alkaline,
trypsin,
pancreatic
enzymes
analyze
amino
acid
composition,
antioxidant
activity,
functional,
physiological
properties.
After
the
extraction
preparation
of
bioactive
peptides
(BAPs)
derived
from
protein,
they
were
enzymatically
hydrolyzed
mentioned
enzymes,
degree
(DH)
was
evaluated.
results
showed
that
samples
fabricated
with
trypsin
alkaline
had
highest
water-holding
capacity
(WHC)
(3.8
g
water/g
dry
matter)
oil-holding
(OHC)
(5.7
oil/g
matter),
respectively.
Enzymatic
enzyme
strongest
radical
scavenging
activities
DPPH
(83.2%)
ABTS
(77.5%),
Additionally,
hydroxyl
activity
(HRSA)
reducing
power
crude
increased
after
hydrolysis.
also
metal-chelating
activity.
Based
on
these
findings,
it
can
be
concluded
has
best
potential
in
improving
nutritional
functional
properties
food
ingredients
products.
PLoS ONE,
Год журнала:
2024,
Номер
19(8), С. e0305348 - e0305348
Опубликована: Авг. 14, 2024
The
emergence
of
antimicrobial
resistance
has
led
to
an
urgent
need
for
novel
drugs.
This
study
aimed
determine
the
antioxidant
and
potentials
in
silico
vitro
Pandanus
amaryllifolius
Roxb.
ethanolic
extract.
extracts
were
subjected
gas
chromatography-mass
spectrometry
(GC-MS)
analysis
identify
compounds.
In
studies
performed
gain
insights
into
possible
mechanism
action
active
compounds
as
antimicrobials.
activities
assessed
using
agar
well
diffusion
method
against
Surabaya
strain
Escherichia
coli
Staphylococcus
aureus
.
Antioxidant
properties
extract
done
DPPH
(2,2-diphenyl-1-picryl-hydrazyl-hydrate)
ABTS
[2,2’-azino-bis
(3-ethylbenzthiazoline-6-sulphonic
acid)]
inhibition
assays.
phytochemical
screening
revealed
that
high
flavonoids
polyphenols
contents.
GC-MS
detected
presence
52
bioactive
substances,
with
n
-hexadecanoic
acid,
9,
12,
15-octadecatrienoic
benzofuran
2,3-dihydro-.
quinic
neophytadiene
major
compound.
Molecular
docking
showed
these
have
a
binding
affinity
towards
target
proteins,
thereby
inhibiting
their
activities.
P
exhibited
IC
50
11.96
±
4.01
μg/ml
26.18
7.44
ABTS.
diameters
zones
(DIZ)
percentage
(PI)
calculated
varied
every
single
pathogen
16.44
1.21mm/66.76
4.92%
(50%)
21.22
0.11mm/82.49
3.91%
E
S
(DIZ/PI)
respectively.
Overall,
this
provides
information
on
responsible
natural
antimicrobe
lays
foundation
further
isolate
characterize
candidates.
Pharmacognosy Journal,
Год журнала:
2022,
Номер
14(4), С. 267 - 272
Опубликована: Сен. 5, 2022
The
global
pandemic
of
COVID-19
has
caused
disastrous
consequences
for
both
humans
and
the
economy.
purpose
this
study
was
to
determine
potential
juwet
(Syzygium
cumini
L.)
moringa
(Moringa
oleifera
as
inhibitors
RBD
spike,
helicase,
Mpro,
RdRp
activity
SARS-CoV-2
with
an
in-silico
approach.
Samples
were
obtained
from
PubChem
RSCB
PDB
databases.
drug
similarity
analysis
determined
using
Swiss
ADME
Lipinski
rule
five.
Prediction
antivirus
probabilities
is
carried
out
PASS
Online.
Molecular
screening
performed
by
molecular
docking
PyRx.
Visualization
used
PyMol
Discovery
Studio.
bioactive
compounds
best
antiviral
had
lowest
affinity
bonds
target
proteins
against
SARS-CoV-2.
Results
show
that
ellagic
acid
java
plum
myricetin
have
antivirals.
However,
more
research
required
validate
results
these
computational
predictions.
Pharmacognosy Journal,
Год журнала:
2022,
Номер
14(4), С. 352 - 357
Опубликована: Сен. 5, 2022
Severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2)
causes
the
COVID-19
pandemic
that
infects
humans
and
attacks
body's
immune
system.
The
purpose
of
study
was
to
identify
potential
bioactive
compounds
in
purslane
(Portulaca
oleracea
L.)
star
anise
(Illicium
verum
Hook)
via
a
dual
inhibitor
mechanism
against
SARS-CoV-2
proteases
with
an
silico
approach.
samples
were
obtained
from
PubChem
RSCB
PDB.
Antivirus
probability
prediction
performed
on
PASS
Online.
Virtual
screening
PyRx
molecular
docking.
Visualization
used
by
PyMol
Discovery
Studio.
Compounds
best
antiviral
are
indicated
low
binding
affinity
value
target
proteins,
namely
TMPRSS2
PLpro.
results
showed
luteolin
has
potential.
However,
further
studies
required
validate
this
computational
prediction.
Frontiers in Microbiology,
Год журнала:
2023,
Номер
14
Опубликована: Июнь 28, 2023
Mosquitoes
are
the
primary
vector
for
West
Nile
virus,
a
flavivirus.
The
virus’s
ability
to
infiltrate
and
establish
itself
in
increasing
numbers
of
nations
has
made
it
persistent
threat
public
health
worldwide.
Despite
widespread
occurrence
this
potentially
fatal
disease,
no
effective
treatment
options
currently
on
market.
As
result,
there
is
an
immediate
need
research
development
novel
pharmaceuticals.
To
begin,
molecular
docking
was
performed
two
possible
virus
target
proteins
using
panel
twelve
natural
chemicals,
including
Apigenin,
Resveratrol,
Hesperetin,
Fungisterol,
Lucidone,
Ganoderic
acid,
Curcumin,
Kaempferol,
Cholic
Chlorogenic
Pinocembrin,
Sanguinarine.
methyltransferase
(PDB
ID:
2OY0)
binding
affinities
varied
from
−7.4
−8.3
kcal/mol,
whereas
envelope
glycoprotein
ranged
−6.2
−8.1
kcal/mol
2I69).
Second,
substances
with
larger
weights
less
likely
be
unhappy
Lipinski
rule.
Hence,
additional
carried
out
without
regard
weight.
In
addition,
compounds
01,
02,
03,
05,
06,
07,
08,
09,
10
11
more
soluble
water
than
compound
04
is.
Besides,
based
maximum
affinity,
best
three
(Apigenin,
Acid)
been
dynamic
simulation
(MDs)
at
100
ns
determine
their
stability.
MDs
data
also
reported
that
these
mentioned
molecules
highly
stable.
Finally,
advanced
principal
component
analysis
(PCA),
dynamics
cross-correlation
matrices
(DCCM)
analysis,
free
energy
cross
correlation
matrix
theoretical
study
included
established
phytochemical
as
potential
drug
candidate.
Research
indicated
aforementioned
may
tool
battle
against
dangerous
virus.
This
aims
locate
bioactive
might
used
pharmaceutical.
Pharmacognosy Journal,
Год журнала:
2022,
Номер
14(5), С. 565 - 574
Опубликована: Ноя. 1, 2022
The
aim
of
this
study
is
to
screen
the
content
bioactive
compounds
Moringa
oleifera
and
identify
its
potential
as
an
antiviral
against
COVID
19
through
entry
inhibitor
mechanism
using
bioinformatics
tools.
sample
was
obtained
from
PubChem
database.
Amino
acis
sequences
were
NCBI.
Protein
modeling
made
SWISSMODEL
site.
target
proteins
for
SARS-CoV-2
Mpro
RdRp.
protein-inhibitory
interaction
drug
M.
predicted
by
molecular
docking
with
PyRx
software.
result
shows
that
a
candidate
compound,
especially
quercetin.
RFMS
value
both
interactions
between
quercetion
RdRp
quercetin
not
higher
than
1.05.
This
still
needed
further
research
prove
prediction.
Pharmacognosy Journal,
Год журнала:
2023,
Номер
14(6), С. 789 - 795
Опубликована: Янв. 9, 2023
Conventional
chemotherapy
substances
are
associated
with
mild
to
severe
side
effects
that
affect
both
healthy
and
cancer
cells.
It
is
presumed
improve
therapeutic
efficacy
in
coexistence
reducing
chemotherapy’s
effects.
Fucoidan
an
anticancer
bioactive
compound
derived
from
Sargassum
sp.
has
low
cytotoxic
activity.
The
purpose
of
this
study
was
explore
the
effectiveness
activities
fucoidan
against
breast
then
analyze
suitability
nano
transdermal
patch
blueprint
long-term
research
design
as
a
chemoprevention
agent
chemotherapeutic
management
cancer.
This
performed
through
literature
silico
by
imposing
carbonic
anhydrase
IX
(CA
IX)
marker
hypoxia
metastatic
state
results
showed
effectively
induced
apoptosis
prevented
metastasis
cells
Bcl-2,
Bcl-w,
bad
pathways.
Fucoidan,
addition,
predicted
inhibit
CA
Glu4
Glu5,
Leu7,
Pro8,
Asp6
residues.
Therefore,
delivery
favored
have
local
effect
on
site
preparations
using
nanoparticle
polymer.
Further
development
treatment
for
suggested
stages
formulation
optimization,
optimum
formula
activity
testing,
patent
filing,
distribution
health
services.
