Preparation of a universally usable, animal product free, defined medium for 2D and 3D culturing of normal and cancer cells

MethodsX, Год журнала: 2024, Номер 12, С. 102592 - 102592

Опубликована: Фев. 3, 2024

Since 1958, cell culture media supplemented with fetal bovine serum is used, despite the well-known concerns about animal welfare, reproducibility, reliability, relevance, and safety. To obliterate these increase scientific accuracy, we recently published an open access, publicly available paper on a defined medium composition to make it possible for any lab prepare this medium. The supports routine culturing banking as well investigations of growth curves, dose response testing compounds cells in 2D 3D, migration; all important aspects research toxicology. Here give detailed description how mix universal 14 simple steps support entity that wishes it. We also list different normal cancer lines have been cultured medium.•Open source product free medium•Protocols mixing solutions small xeno molecules supplementation•Protocols human proteins supplementation.

Язык: Английский

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Viral Metagenomics as a Tool to Track Sources of Fecal Contamination: A One Health Approach

Viruses, Год журнала: 2023, Номер 15(1), С. 236 - 236

Опубликована: Янв. 14, 2023

The One Health framework recognizes that human, animal, and environmental health are linked highly interdependent. Fecal contamination of water, soil, foodstuff, air may impact many aspects Health, culture, PCR-based, sequencing methods utilized in the detection fecal to determine source, load, risk inform targeted mitigation strategies. Viruses, particularly, have been considered as indicators given narrow host range exhibit their association with other biological contaminants. Culture- molecular-based gold-standards for virus determining specific sources via viral indicators. However, metagenomics is also being a tool tracking contamination. In present review, studies potential freshwaters, marine waters, using discussed highlight optimizing source tracking. Limitations use track sources, including sample processing, nucleic acid recovery, depth, bioinformatics discussed. Finally, review discusses part toolbox approach.

Язык: Английский

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A Diverse Virome Is Identified in Parasitic Flatworms of Domestic Animals in Xinjiang, China

Microbiology Spectrum, Год журнала: 2023, Номер 11(3)

Опубликована: Апрель 12, 2023

Parasitic flatworms infect diverse vertebrates and are major threats to animal even human health; however, little is known about the virome of these lower life forms. Using viral metagenomic sequencing, we characterized parasitic collected from domestic animals, including Dicrocoelium lanceatum Taenia hydatigena, Echinococcus granulosus sensu stricto multilocularis. Seven three different viruses were discovered D. T. respectively, no sequences found in adult tapeworms protoscoleces E. Two out five flatworm species carry viruses, showing a host specificity viruses. These belong Parvoviridae, Circoviridae, unclassified circular, Rep-encoding single-stranded (CRESS) DNA virus, Rhabdoviridae, Endornaviridae, RNA The presence multiple highly divergent especially those that cluster with marine implies deep evolutionary history parasite-associated In addition, high identity common pathogens dogs, canine circovirus parvovirus 2. parasites they may but does not completely exclude possibility contamination intestinal contents. Furthermore, demonstrated certain such as CRESS virus integrate into genome their host. Our results expand knowledge diversity important highlighting need for further investigations prevalence among other key animals. IMPORTANCE Characterizing unveiling diversity, evolution, ecology will help understand "Russian doll" pattern parasites, data indicate present specific flatworms, have an ancient currently circulating parasite-infected also raise question whether acquire and/or some transmission potential through study virus-parasite-host interactions, influence infection on cycle parasite, well its fitness pathogenicity host, could find new strategies prevent control diseases.

Язык: Английский

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Food safety of fermented proteins and cultivated meat and seafood

Elsevier eBooks, Год журнала: 2024, Номер unknown, С. 77 - 94

Опубликована: Янв. 1, 2024

Язык: Английский

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Unmapped short reads from whole-genome sequencing indicate potential infectious pathogens in German Black Pied cattle

Veterinary Research, Год журнала: 2023, Номер 54(1)

Опубликована: Окт. 18, 2023

When resequencing animal genomes, some short reads cannot be mapped to the reference genome and are usually discarded. In this study, unmapped from 302 German Black Pied cattle were analyzed identify potential pathogenic DNA. These assembled blasted against NCBI's database bacterial viral sequences. The results provided evidence for presence of pathogens. We found sequences Bovine parvovirus 3 Mycoplasma species. findings emphasize information content gaining insight into infections, which is important veterinarians epidemiologists.

Язык: Английский

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BOVINE PESTIVIRUSES AS CONTAMINANTS OF BIOLOGICAL PREPARATIONS (review)

Sel skokhozyaistvennaya Biologiya, Год журнала: 2024, Номер 59(2), С. 179 - 193

Опубликована: Апрель 1, 2024

К таким вирусам относятся прототипный вид Pestivirus А (вирус вирусной диареи крупного рогатого скота 1 вида; BVDV-1), В 2 BVDV-2) и H (Hobi-like pestivirus, HoBiPeV; вирус

Язык: Русский

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Research Progress of Information Technology Applied to the Management of Pulmonary Tuberculosis Patients

Advances in Clinical Medicine, Год журнала: 2023, Номер 13(06), С. 8871 - 8876

Опубликована: Янв. 1, 2023

Язык: Английский

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