Biology,
Год журнала:
2021,
Номер
10(2), С. 91 - 91
Опубликована: Янв. 26, 2021
Severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2)
has
caused
a
worldwide
crisis
with
profound
effects
on
both
public
health
and
the
economy.
In
order
to
combat
COVID-19
pandemic,
research
groups
have
shared
viral
genome
sequence
data
through
Global
Initiative
Sharing
All
Influenza
Data
(GISAID).
Over
past
year,
≈290,000
full
SARS-CoV-2
proteome
sequences
been
deposited
in
GISAID.
Here,
we
used
these
assess
rate
of
nonsynonymous
mutants
over
entire
proteome.
Our
analysis
shows
that
proteins
are
mutating
at
substantially
different
rates,
most
exhibiting
little
mutational
variability.
As
anticipated,
our
calculations
capture
previously
reported
mutations
arose
first
months
such
as
D614G
(Spike),
P323L
(NSP12),
R203K/G204R
(Nucleocapsid),
but
they
also
identify
more
recent
mutations,
A222V
L18F
(Spike)
A220V
among
others.
comprehensive
temporal
geographical
analyses
show
two
distinct
periods
mutation
rates:
December
2019
July
2020
August
2020.
Notably,
some
rates
differ
by
geography,
primarily
during
latter
half
Europe.
Furthermore,
structure-based
molecular
provides
an
exhaustive
assessment
context
current
set
3D
structures
available
for
proteins.
This
emerging
sequence-to-structure
insight
is
beginning
illuminate
site-specific
(in)tolerance
virus
continues
spread
around
globe.
The
COVID-19
pandemic
is
a
harsh
reminder
of
the
fact
that,
whether
in
single
human
host
or
wave
infection
across
continents,
viral
dynamics
often
story
about
numbers.
In
this
article
we
provide
one-stop,
curated
graphical
source
for
key
numbers
(based
mostly
on
peer-reviewed
literature)
SARS-CoV-2
virus
that
responsible
pandemic.
discussion
framed
around
two
broad
themes:
i)
biology
itself;
ii)
characteristics
host.
Journal of Cellular Physiology,
Год журнала:
2021,
Номер
236(10), С. 7045 - 7057
Опубликована: Март 23, 2021
Abstract
The
evolution
of
the
SARS‐CoV‐2
new
variants
reported
to
be
70%
more
contagious
than
earlier
one
is
now
spreading
fast
worldwide.
There
an
instant
need
discover
how
interact
with
host
receptor
(ACE2).
Among
mutations
in
Spike
glycoprotein
variants,
three
are
specific
receptor‐binding
domain
(RBD)
and
required
insightful
scrutiny
for
therapeutic
options.
These
structural
evolutions
RBD
may
impart
a
critical
role
unique
pathogenicity
variants.
Herein,
using
biophysical
approaches,
we
explored
that
UK
(N501Y),
South
African
(K417N‐E484K‐N501Y),
Brazilian
(K417T‐E484K‐N501Y),
hypothetical
(N501Y‐E484K)
alter
binding
affinity,
create
inter‐protein
contacts
changes
internal
dynamics
thereby
increases
eventually
infectivity.
Our
investigation
highlighted
(K417T‐E484K‐N501Y)
lethal
variant
(N501Y).
behavior
wild
type
N501Y
comparable.
Free
energy
calculations
further
confirmed
increased
spike
ACE2
mainly
due
electrostatic
contribution.
Further,
find
unusual
virulence
this
virus
potentially
consequence
Darwinian
selection‐driven
epistasis
protein
evolution.
triple
mutants
(South
Brazilian)
pose
serious
threat
efficacy
already
developed
vaccine.
analysis
would
help
understand
demand
vitro
vivo
models
design
potential
therapeutics
against
Proceedings of the National Academy of Sciences,
Год журнала:
2021,
Номер
118(25)
Опубликована: Июнь 3, 2021
Quantitatively
describing
the
time
course
of
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2)
infection
within
an
infected
individual
is
important
for
understanding
current
global
pandemic
and
possible
ways
to
combat
it.
Here
we
integrate
best
knowledge
about
typical
viral
load
SARS-CoV-2
in
bodily
fluids
host
tissues
estimate
total
number
mass
virions
person.
We
that
each
person
carries
109
1011
during
peak
infection,
with
a
range
1
μg
100
μg,
which
curiously
implies
all
currently
circulating
human
hosts
have
collective
only
0.1
kg
10
kg.
combine
our
estimates
available
literature
on
immune
response
mutation
rates
demonstrate
how
antibodies
markedly
outnumber
spike
proteins,
genetic
diversity
covers
single
nucleotide
substitutions.
Frontiers in Cellular and Infection Microbiology,
Год журнала:
2022,
Номер
11
Опубликована: Янв. 18, 2022
The
structural
spike
(S)
glycoprotein
of
severe
acute
respiratory
syndrome
coronavirus-2
(SARS-CoV-2)
plays
an
essential
role
in
infection
and
is
important
target
for
neutralizing
antibody
recognition.
Mutations
the
S
gene
can
generate
variants
concern
(VOCs),
which
improve
"viral
fitness"
through
selective
or
survival
advantages,
such
as
increased
ACE-2
receptor
affinity,
infectivity,
viral
replication,
higher
transmissibility,
resistance
to
antibodies
immune
escape,
increasing
disease
severity
reinfection
risk.
Five
VOCs
have
been
recognized
include
B.1.1.7
(U.K.),
B.1.351
(South
Africa),
P.1
(Brazil),
B.1.617.2
(India),
B.1.1.529
(multiple
countries).
In
this
review,
we
addressed
following
critical
points
concerning
VOCs:
a)
characteristics
SARS-CoV-2
with
mutations
gene;
b)
possible
evasion
from
generated
vaccination,
previous
infection,
therapies;
c)
potential
risk
new
pandemic
waves
induced
by
worldwide;
d)
perspectives
further
studies
actions
aimed
at
preventing
reducing
impact
during
current
COVID-19
pandemic.
Frontiers in Molecular Biosciences,
Год журнала:
2020,
Номер
7
Опубликована: Дек. 17, 2020
Severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2)
has
rapidly
spread
in
humans
almost
every
country,
causing
the
disease
COVID-19.
Since
start
of
COVID-19
pandemic,
research
efforts
have
been
strongly
directed
towards
obtaining
a
full
understanding
biology
viral
infection,
order
to
develop
vaccine
and
therapeutic
approaches.
In
particular,
structural
studies
allowed
comprehend
molecular
basis
underlying
role
many
SARS-CoV-2
proteins,
make
rapid
progress
treatment
preventive
therapeutics.
Despite
great
advances
that
provided
by
these
studies,
knowledge
gaps
on
infection
still
remain.
Filling
will
be
key
tackle
this
through
development
effective
treatments
specific
vaccination
strategies.
