Microbiota and urinary tumor immunity: Mechanisms, therapeutic implications, and future perspectives

Chinese Journal of Cancer Research, Год журнала: 2024, Номер 36(6), С. 596 - 615

Опубликована: Янв. 1, 2024

Язык: Английский

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Gene therapy in polycystic kidney disease: A promising future

Journal of Translational Internal Medicine, Год журнала: 2024, Номер 12(6), С. 543 - 552

Опубликована: Дек. 1, 2024

Polycystic kidney disease (PKD) is a genetic disorder marked by numerous cysts in the kidneys, progressively impairing renal function. It classified into autosomal dominant polycystic (ADPKD) and recessive (ARPKD), with ADPKD being more common. Current treatments mainly focus on symptom relief slowing progression, without offering cure. Recent advancements gene editing technologies, such as CRISPR-Cas9, have introduced new therapeutic possibilities for PKD. These approaches include miR-17 antisense oligonucleotides, adenovirus-mediated knockdown, Pkd1 or polycystin -1 C-terminal tail enhancement therapy, 3-UTR binding element which shown potential animal models early clinical trials. Specifically ARPKD, strategies like oligonucleotide therapy targeting c-myc CRISPR/ Cas9 knockdown of P2rx7 promise. Despite facing challenges technological limitations, ethical legal issues, high costs, presents unprecedented hope PKD treatment. Future interdisciplinary collaboration international cooperation are essential developing effective treatment patients.

Язык: Английский

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Prognostic and diagnostic value of circRNA expression in cervical cancer: a meta analysis

Frontiers in Oncology, Год журнала: 2025, Номер 14

Опубликована: Янв. 13, 2025

Introduction Cervical cancer (CC) is a highly prevalent malignancy of the reproductive system. This study aimed to methodically assess function circular RNAs (circRNAs) as possible indicators CC, with specific emphasis on their usefulness in identification, prediction, and correlation clinicopathological elements. Methods A comprehensive literature search was conducted using databases such PubMed, Cochrane Library, Web Science, Embase, China National Knowledge Infrastructure (CNKI). The latest data were extracted May 3 rd , 2024. diagnostic potential circRNA expression evaluated range metrics including sensitivity, specificity, area under receiver operating characteristic curve (AUC). importance circRNAs further terms clinical relevance, pathological features, prognostic value pooled odds ratios (ORs) hazard (HRs). Results meta-analysis included 27 studies, which categorised based applications (n=3), correlations (n=15), evaluations (n=23). Elevated levels oncogenic significantly associated poor indicators, tumour size (odds ratio [OR] = 0.425, 95% confidence interval [CI]: 0.267–0.676), International Federation Gynaecology Obstetrics (FIGO) stage (OR 0.315, CI: 0.224–0.443), lymph node metastasis 2.975, 1.816–4.872). upregulation also identified predictor worse survival outcomes, (HR) 2.13 (95% 1.73–2.62, P < 0.001). downregulation tumour-suppressor properties similarly parameters, 0.310, 0.102–0.941), FIGO 0.231, 0.101–0.527), 2.430, 1.156–5.110), indicative worsened prognosis (HR 2.20, 1.03–4.70, 0.042). In value, (AUC) calculated be 0.85, 0.83, 0.91, respectively. Conclusion results our indicate that have serve promising biomarkers for CC diagnosis, prognosis, clinicopathology. Systematic review registration https://www.crd.york.ac.uk/PROSPERO/ identifier CRD42024544997.

Язык: Английский

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Pingxiao pian attenuate invasiveness and proliferation of lung adenocarcinoma through regulating miR-29b-3p/TGF-β1/Smad/EMT pathway

Discover Oncology, Год журнала: 2025, Номер 16(1)

Опубликована: Фев. 24, 2025

Lung cancer is a highly prevalent and deadly disease worldwide, causing over 1.2 million deaths each year. Pingxiao Pian (PXP) tablets, Chinese traditional medicine, have been widely applied in the treatment of lung cancer. However, mechanism underlying therapeutic effects PXP tablets remains undisclosed. A549 human LUAD cell line was utilized for vitro experiments. Transfection miR-29b mimic performed using Lipofectamine 3000. purchased dissolved into PBS drinking water after carefully removing outer coating. Dual-luciferase reporter assay conducted to assess regulatory effect on TGF-β1. The protein levels epithelial-mesenchymal transition (EMT) markers activation TGF-β1 pathway were characterized immunoblotting analysis. reduced invasiveness proliferation cells by increasing miR-29b-3p expression vitro. Overexpression resulted decreased invasiveness, while silencing displayed opposite effect. Moreover, reversed increased proliferating rate triggered silencing. Additionally, found hamper EMT occurrence regulating reduce N-cad vimentin. blocked phosphorylation Smad2/3 expression. Luciferase results indicated that directly regulated In vivo tumor formation experiments confirmed tumor-reducing role progression. size weight via miR-29b-3p. Our study suggests exerts anti-tumor through regulation inhibition TGF-β1/Smad2/3 pathway.

Язык: Английский

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Motor protein KIF5B inhibition as a novel strategy of controlled reperfusion against myocardial ischemia/reperfusion injury

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Год журнала: 2025, Номер 1871(5), С. 167785 - 167785

Опубликована: Март 7, 2025

Язык: Английский

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