miR-34a-5p modulation of polycystic ovary syndrome via targeting the NOTCH signaling pathway DOI Creative Commons
Kexin Zhang, Xiaomeng Wang, Fang Liu

и другие.

Journal of Ovarian Research, Год журнала: 2025, Номер 18(1)

Опубликована: Март 13, 2025

Polycystic ovary syndrome (PCOS) is currently recognized as a condition that affects several systems in the body, including reproductive, endocrine, and cardiovascular systems. Prevalent among teenagers women of reproductive age. Prior research has demonstrated an elevation miR-34a-5p within follicular fluid (FF) PCOS. Despite this, precise mechanisms through which influences granulosa cells (GC) development function remain poorly characterized. Therefore, this study investigates involvement pathogenic GCs context The human granulosa-like tumor cell line (KGN) got transfected at control, well mimic inhibitor, respectively. Monitor cellular proliferation each experimental group. methods included RT-qPCR, CCK8, flow cytometry western blotting. Also, interaction between particular sequence JAG1 been verified using dual luciferase assay. Further investigation connection involving Notch signaling pathway was conducted bioinformatics analysis methods. results expression significantly elevated serum(p<0. 0001)and FF (p = 0. 0402) PCOS, whereas its 5522) showed no significant variation. Overexpressing caused decrease rate KGN multiplied increase programmed death. Conversely, inhibiting resulted growth Bioinformatics further thatmiR-34a-5p interacts with 3'UTR region JAG1, leading to negative regulation Jagged1-Notch pathway. In summary, molecule inhibits triggers death by regulating Silencing prevents dysfunction GCs. Our implies new molecular site treat

Язык: Английский

miR-34a-5p modulation of polycystic ovary syndrome via targeting the NOTCH signaling pathway DOI Creative Commons
Kexin Zhang, Xiaomeng Wang, Fang Liu

и другие.

Journal of Ovarian Research, Год журнала: 2025, Номер 18(1)

Опубликована: Март 13, 2025

Polycystic ovary syndrome (PCOS) is currently recognized as a condition that affects several systems in the body, including reproductive, endocrine, and cardiovascular systems. Prevalent among teenagers women of reproductive age. Prior research has demonstrated an elevation miR-34a-5p within follicular fluid (FF) PCOS. Despite this, precise mechanisms through which influences granulosa cells (GC) development function remain poorly characterized. Therefore, this study investigates involvement pathogenic GCs context The human granulosa-like tumor cell line (KGN) got transfected at control, well mimic inhibitor, respectively. Monitor cellular proliferation each experimental group. methods included RT-qPCR, CCK8, flow cytometry western blotting. Also, interaction between particular sequence JAG1 been verified using dual luciferase assay. Further investigation connection involving Notch signaling pathway was conducted bioinformatics analysis methods. results expression significantly elevated serum(p<0. 0001)and FF (p = 0. 0402) PCOS, whereas its 5522) showed no significant variation. Overexpressing caused decrease rate KGN multiplied increase programmed death. Conversely, inhibiting resulted growth Bioinformatics further thatmiR-34a-5p interacts with 3'UTR region JAG1, leading to negative regulation Jagged1-Notch pathway. In summary, molecule inhibits triggers death by regulating Silencing prevents dysfunction GCs. Our implies new molecular site treat

Язык: Английский

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