Multistep Enzymatic Low-density Polyethylene Degradation via Phenylalanine Monooxygenase and Isocitrate Lyase in Pseudomonas aeruginosa DOI Creative Commons
Hong Rae Kim,

Ye Eun Lee,

Dong-Eun Suh

и другие.

Опубликована: Июнь 4, 2024

Plastics have become indispensable in modern industries; however, their resistance to natural degradation poses environmental challenges. Biological technologies employing microorganisms offer promising solutions. Here, we analyzed the transcriptome and proteome of Pseudomonas aeruginosa, a plastic-degrading microorganism found gut superworms, identify genes enzymes upregulated during polyethylene degradation. Functional analyses these using Kyoto Encyclopedia Genes Genomes Gene Ontology databases revealed an increase lipid hydrophobic amino acid metabolism, suggesting involvement Based on analyses, identified phenylalanine monooxygenase, which is capable oxidizing plastics, isocitrate lyase, involved C-C bond cleavage. To investigate degradation, phhA aceA were transformed into Escherichia coli, produced purified. The purified then reacted with analyzed. results formation hydroxyl (-OH) C-O groups surface after treatment confirming its ability oxidize polyethylene. Isocitrate lyase alone did not affect production; when combined it contributed reduction molecular weight. This suggests two-stage process involving oxidation depolymerization that requires sequential action multiple enzymes. Thus, each stage demonstrated

Язык: Английский

Multistep Enzymatic Low-density Polyethylene Degradation via Phenylalanine Monooxygenase and Isocitrate Lyase in Pseudomonas aeruginosa DOI Creative Commons
Hong Rae Kim,

Ye Eun Lee,

Dong-Eun Suh

и другие.

Опубликована: Июнь 4, 2024

Plastics have become indispensable in modern industries; however, their resistance to natural degradation poses environmental challenges. Biological technologies employing microorganisms offer promising solutions. Here, we analyzed the transcriptome and proteome of Pseudomonas aeruginosa, a plastic-degrading microorganism found gut superworms, identify genes enzymes upregulated during polyethylene degradation. Functional analyses these using Kyoto Encyclopedia Genes Genomes Gene Ontology databases revealed an increase lipid hydrophobic amino acid metabolism, suggesting involvement Based on analyses, identified phenylalanine monooxygenase, which is capable oxidizing plastics, isocitrate lyase, involved C-C bond cleavage. To investigate degradation, phhA aceA were transformed into Escherichia coli, produced purified. The purified then reacted with analyzed. results formation hydroxyl (-OH) C-O groups surface after treatment confirming its ability oxidize polyethylene. Isocitrate lyase alone did not affect production; when combined it contributed reduction molecular weight. This suggests two-stage process involving oxidation depolymerization that requires sequential action multiple enzymes. Thus, each stage demonstrated

Язык: Английский

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