Coiled double amyloid-like fibrils allosterically catalyze hydrolysis of beta-lactam antibiotics DOI Creative Commons
Raz Jelinek,

sisira Mambram Kunnath,

Elad Arad

и другие.

Опубликована: Авг. 8, 2024

The release of antibiotic compounds into wastewater constitutes a significant and growing health environmental hazard, particularly contributing to the spread resistant bacterial strains. Here, we demonstrate that amyloid fibrils, consisting an alternating lysine/phenylalanine -sheet forming short peptide, catalyze hydrolysis β-lactam antibiotics, most prominent family compounds, which is further widespread in wastewater. Peptide variant analysis, molecular dynamics (MD) simulations, cryogenic electron microscopy (cryo-EM) reveal molecules dock onto fibrils’ surface via electrostatic interactions with lysine sidechains. Importantly, catalytic occurs allosteric mechanism mediated by unique coiled double fibril structure anchored are embedded within twisted fiber strands, facilitating nucleophilic attacks Utilization lysine-displaying fibrils for hydrolytic degradation removal antibiotics from water was accomplished through display on silica beads placed conventional column filtration setup. Amyloid displaying arrays may furnish versatile platform water, underscoring new avenues addressing considerable threat contamination.

Язык: Английский

Coiled double amyloid-like fibrils allosterically catalyze hydrolysis of beta-lactam antibiotics DOI Creative Commons
Raz Jelinek,

sisira Mambram Kunnath,

Elad Arad

и другие.

Опубликована: Авг. 8, 2024

The release of antibiotic compounds into wastewater constitutes a significant and growing health environmental hazard, particularly contributing to the spread resistant bacterial strains. Here, we demonstrate that amyloid fibrils, consisting an alternating lysine/phenylalanine -sheet forming short peptide, catalyze hydrolysis β-lactam antibiotics, most prominent family compounds, which is further widespread in wastewater. Peptide variant analysis, molecular dynamics (MD) simulations, cryogenic electron microscopy (cryo-EM) reveal molecules dock onto fibrils’ surface via electrostatic interactions with lysine sidechains. Importantly, catalytic occurs allosteric mechanism mediated by unique coiled double fibril structure anchored are embedded within twisted fiber strands, facilitating nucleophilic attacks Utilization lysine-displaying fibrils for hydrolytic degradation removal antibiotics from water was accomplished through display on silica beads placed conventional column filtration setup. Amyloid displaying arrays may furnish versatile platform water, underscoring new avenues addressing considerable threat contamination.

Язык: Английский

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