Abstract
In
this
study,
the
transcriptional
repressor
REST
(Repressor
Element
1
Silencing
Transcription
factor)
was
ablated
in
mouse
placenta
to
investigate
molecular
and
cellular
impacts
on
offspring
brain
at
different
life
stages.
Ablation
of
placental
deregulated
several
metabolites,
including
glucose
lactate
that
fuel
energy,
vitamin
C
(ascorbic
acid)
functions
epigenetic
programming
during
postnatal
development,
glutamate
creatine
help
respond
stress
conditions
adult
life.
Bulk
RNA‐seq
analysis
showed
a
lack
persistently
altered
multiple
transport
genes,
those
related
oxygen
transportation
brain.
While
metabolic
genes
were
impacted
brain,
response
activated
DNA
methylation
also
due
loss
REST,
but
sex‐biased
manner.
Single‐nuclei
specific
cell
types
particularly
choroid
plexus
ependyma,
which
play
critical
roles
producing
cerebrospinal
fluid
maintaining
homeostasis,
significantly
REST.
These
cells
significant
differential
expression
associated
with
metabotropic
(G
coupled
protein)
ionotropic
(ligand‐gated
ion
channel)
receptors,
suggesting
an
impact
ablation
glutamatergic
signaling
The
study
expands
our
understanding
influences
Molecular Psychiatry,
Год журнала:
2022,
Номер
27(9), С. 3573 - 3582
Опубликована: Май 26, 2022
Abstract
The
choroid
plexus
(CP)
is
a
delicate
and
highly
vascularized
structure
in
the
brain
comprised
of
dense
network
fenestrated
capillary
loops
that
help
synthesis,
secretion
circulation
cerebrospinal
fluid
(CSF).
This
unique
neuroanatomical
arachnoid
villi
stemming
from
frond-like
surface
projections—that
protrude
into
lumen
four
cerebral
ventricles—providing
key
source
nutrients
to
parenchyma
addition
serving
as
‘sink’
for
central
nervous
system
metabolic
waste.
In
fact,
functions
CP
are
often
described
being
analogous
those
liver
kidney.
Beyond
forming
barrier/interface
between
blood
CSF
compartments,
has
been
identified
modulator
leukocyte
trafficking,
inflammation,
cognition,
circadian
rhythm
gut
brain-axis.
recent
years,
advances
molecular
biology
techniques
neuroimaging
along
with
use
sophisticated
animal
models
have
played
an
integral
role
shaping
our
understanding
how
CP–CSF
changes
relation
maturation
neural
circuits
during
critical
periods
development.
this
article
we
provide
ontogenetic
perspective
review
experimental
evidence
implicating
pathophysiology
neurodevelopmental
neuropsychiatric
disorders.
Fluids and Barriers of the CNS,
Год журнала:
2022,
Номер
19(1)
Опубликована: Май 26, 2022
Choroid
plexus
(ChP)
epithelial
cells
are
crucial
for
the
function
of
blood-cerebrospinal
fluid
barrier
(BCSFB)
in
developing
and
mature
brain.
The
ChP
is
considered
primary
source
regulator
CSF,
secreting
many
important
factors
that
nourish
It
also
performs
CSF
clearance
functions
including
removing
Amyloid
beta
potassium.
As
such,
a
promising
target
gene
drug
therapy
neurodevelopmental
neurological
disorders
central
nervous
system
(CNS).
This
review
describes
current
successful
emerging
experimental
approaches
targeting
cells.
We
highlight
methodological
strategies
to
specifically
these
gain
or
loss
vivo.
cover
both
genetic
models
viral
delivery
systems.
Additionally,
several
lines
reporters
access
epithelia
reviewed.
Finally,
we
discuss
exciting
new
approaches,
such
as
chemical
activation
transplantation
engineered
elaborate
on
fundamental
secretion
outline
paving
way
clinical
applications.
Cells and Development,
Год журнала:
2023,
Номер
174, С. 203841 - 203841
Опубликована: Апрель 14, 2023
In
the
adult
rodent
brain,
neural
stem
cells
(NSCs)
reside
in
subventricular
zone
(SVZ)
of
lateral
ventricles
and
subgranular
(SGZ)
hippocampus.
these
areas,
NSCs
their
progeny
integrate
intrinsic
signals
extrinsic
cues
provided
by
microenvironment
that
control
behavior.
The
vasculature
SVZ
SGZ,
choroid
plexus
(ChP)
SVZ,
have
emerged
as
critical
compartments
neurogenic
niches
they
provide
a
rich
repertoire
to
regulate
NSC
quiescence,
proliferation,
self-renewal
differentiation.
Physical
contact
between
blood
vessels
is
also
feature
within
supports
different
processes
such
migration
vesicle
transport.
this
review,
we
description
brain
both
cell
niches,
highlighting
main
properties
role
each
niche.
We
summarize
current
understanding
how
vessel-
ChP-derived
influence
behavior
physiological
adulthood,
well
upon
aging.
Cellular and Molecular Life Sciences,
Год журнала:
2022,
Номер
79(6)
Опубликована: Май 19, 2022
Abstract
The
choroid
plexus
(ChP)
is
an
extensively
vascularized
tissue
that
protrudes
into
the
brain
ventricular
system
of
all
vertebrates.
This
highly
specialized
structure,
consisting
polarized
epithelial
sheet
and
underlying
stroma,
serves
a
spectrum
functions
within
central
nervous
(CNS),
most
notably
production
cerebrospinal
fluid
(CSF).
cells
ChP
have
competence
to
tightly
modulate
biomolecule
composition
CSF,
which
acts
as
milieu
functionally
connecting
with
other
structures.
review
aims
eloquently
summarize
current
knowledge
about
development
ChP.
We
describe
mechanisms
control
its
early
specification
from
roof
plate
followed
by
formation
proliferative
regions—cortical
hem
rhombic
lips—feeding
later
Next,
we
summarized
on
maturation
morphological
cellular
diversity.
Furthermore,
attempted
currently
available
battery
molecular
markers
mouse
strains
for
research
ChP,
identified
some
technological
shortcomings
must
be
overcome
accelerate
field.
Overall,
principle
this
highlight
intriguing
surprisingly
poorly
known
structure
vital
function
whole
CNS.
believe
our
summary
will
increase
interest
in
further
studies
aim
principles
guiding
tissue.
International Journal of Molecular Sciences,
Год журнала:
2020,
Номер
21(13), С. 4760 - 4760
Опубликована: Июль 4, 2020
Cerebrospinal
fluid
(CSF)
is
the
liquid
that
fills
brain
ventricles.
CSF
represents
not
only
a
mechanical
protection
but
also
rich
source
of
signalling
factors
modulating
diverse
processes
during
development
and
adulthood.
The
choroid
plexus
(CP)
major
as
such
it
has
recently
emerged
an
important
mediator
extracellular
within
brain.
Growing
interest
in
CP
revealed
its
capacity
to
release
broad
variety
bioactive
molecules
that,
via
CSF,
regulate
across
whole
central
nervous
system
(CNS).
Moreover,
been
recognized
sensor,
responding
altered
composition
associated
with
changes
patterns
CNS
activity.
In
this
review,
we
summarize
recent
advances
our
understanding
centre
mediates
long-range
communication
CNS.
By
providing
detailed
account
secretory
repertoire,
describe
how
contributes
regulation
environment—in
context
both
embryonal
well
adult
We
highlight
role
regulator
function
acts
CSF-mediated
signalling.
Further
studies
CP–CSF
hold
potential
provide
key
insights
into
biology
CNS,
implications
for
better
treatment
neuropathological
conditions.
Journal of Tissue Engineering,
Год журнала:
2024,
Номер
15
Опубликована: Янв. 1, 2024
In
vitro
modeling
of
brain
tissue
is
a
promising
but
not
yet
resolved
problem
in
modern
neurobiology
and
neuropharmacology.
Complexity
the
structure
diversity
cell-to-cell
communication
(patho)physiological
conditions
make
this
task
almost
unachievable.
However,
establishment
novel
models
would
ultimately
lead
to
better
understanding
development-associated
or
experience-driven
plasticity,
designing
efficient
approaches
restore
aberrant
functioning.
The
main
goal
review
summarize
available
data
on
methodological
that
are
currently
use,
identify
most
prospective
trends
development
neurovascular
unit,
blood-brain
barrier,
blood-cerebrospinal
fluid
neurogenic
niche
models.
manuscript
focuses
regulation
adult
neurogenesis,
cerebral
microcirculation
fluids
dynamics
should
be
reproduced
4D
mimic
its
alterations
pathology.
We
discuss
critical
for
studying
deciphering
individual
person-specific
trajectory
aging,
testing
new
drug
candidates
International Journal of Molecular Sciences,
Год журнала:
2021,
Номер
22(16), С. 8951 - 8951
Опубликована: Авг. 19, 2021
The
choroid
plexus
is
an
important
blood
barrier
that
secretes
cerebrospinal
fluid,
which
essential
for
embryonic
brain
development
and
adult
homeostasis.
OTX2
homeoprotein
a
transcription
factor
critical
remains
highly
expressed
in
plexus.
Through
RNA
sequencing
analyses
of
constitutive
conditional
knockdown
mouse
models,
we
reveal
putative
functional
roles
function,
including
cell
signaling
adhesion,
show
regulates
the
expression
factors
are
secreted
into
notably
transthyretin.
We
also
Otx2
impacts
immune
stress
responses,
affects
splicing,
leading
to
changes
mRNA
isoforms
proteins
implicated
oxidative
response
DNA
repair.
mass
spectrometry
analysis
protein
partners
plexus,
known
non-cell-autonomous
target
regions,
such
as
visual
cortex
subventricular
zone,
identify
targets
involved
chromatin
structure,
processing.
Thus,
retains
regulating
function
homeostasis
throughout
life.
Schizophrenia Research,
Год журнала:
2024,
Номер
275, С. 107 - 114
Опубликована: Дек. 17, 2024
The
choroid
plexus
is
an
important
structure
within
the
ventricular
system.
Schizophrenia
has
been
associated
with
morphological
changes
to
but
presence
and
extent
of
alterations
at
different
illness
stages
unclear.
We
examined
volumes
in
participants
clinical
high-risk
for
psychosis
(N
=
110),
first-episode
37),
schizophrenia
28),
38)
non-clinical
controls
75).
Automated
segmentation
(Gaussian
mixture
model)
was
used
estimate
from
T1
magnetic
resonance
(MR)
images.
then
conducted
a
linear
model
Bayes
factor
analysis
investigate
group
differences.
In
addition,
relationship
between
characteristics
assessed.
patients
were
characterized
by
increased
volume
while
showed
no
differences
volumes.
However,
did
not
significantly
differ
when
controlling
volume.
Finally,
group.
Our
findings
suggest
that
are
specific
early-stage
psychosis.
Previously
reported
abnormalities
could
be
explained
Fluids and Barriers of the CNS,
Год журнала:
2022,
Номер
19(1)
Опубликована: Окт. 15, 2022
In
the
vascular
compartment,
serine
protease
tissue-type
plasminogen
activator
(tPA)
promotes
fibrinolysis,
justifying
its
clinical
use
against
vasculo-occlusive
diseases.
Accumulating
evidence
shows
that
circulating
tPA
(endogenous
or
exogenous)
also
controls
brain
physiopathological
processes,
like
cerebrovascular
reactivity,
blood-brain
barrier
(BBB)
homeostasis,
inflammation
and
neuronal
fate.
Whether
this
occurs
by
direct
actions
on
parenchymal
cells
and/or
indirectly
via
barriers
between
blood
central
nervous
system
(CNS)
remains
unclear.
Here,
we
postulated
can
reach
parenchyma
blood-cerebrospinal
fluid
(BCSFB),
relies
choroid
plexus
(CP)
epithelial
(CPECs).We
produced
various
reporter
fusion
proteins
to
track
in
primary
cultures
of
CPECs,
CP
explants
vivo
mice.
We
investigated
mechanisms
underlying
transport
across
BCSFB,
with
pharmacological
molecular
approaches.We
first
demonstrated
be
internalized
CPECs
ex
CPs
explants.
vivo,
both
at
their
basal
apical
sides.
After
intra-vascular
administration,
cerebral
spinal
(CSF)
parenchyma.
Further
investigation
allowed
discovering
transcytosis
is
mediated
Low-density-Lipoprotein
Related
Protein-1
(LRP1)
expressed
surface
depends
finger
domain
tPA.
Interestingly,
albumin,
which
has
a
size
comparable
tPA,
does
not
normally
cross
CPs,
but
switches
transportable
form
when
grafted
tPA.These
findings
provide
new
insights
how
parenchyma,
open
therapeutic
avenues
for
CNS
disorders.