Stem Cell-Derived Organoids, Embryoids, and Embryos: Advances in Organismic Development In Vitro Force Us to Re-Focus on Ethical and Legal Aspects of Model Choice DOI Creative Commons
Hans‐Werner Denker

Organoids, Год журнала: 2023, Номер 2(4), С. 231 - 238

Опубликована: Дек. 5, 2023

While research on stem cell-derived tissues and organoids is rapidly expanding, the technically related creation of complex embryoids has recently excited a vivid discussion since it raises ethical questions about individuation possible gain viability. The present study focuses onset organismic development proposed biological legal definitions for terms embryo, embryoid, organoid. It concluded that such considerations have become important investigators’ choices appropriate in vitro model systems, allowing formation vs. embryoids.

Язык: Английский

Totipotent-like reprogramming: Molecular machineries and chemical manipulations DOI

Wei Cai,

Lingci Huang,

Xiaoping Wu

и другие.

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Год журнала: 2025, Номер 1872(3), С. 119925 - 119925

Опубликована: Фев. 22, 2025

Язык: Английский

Процитировано

1

Ovarian Stem Cells: Will the Dream of Neo-Folliculogenesis After Birth Become Real? DOI
Stefano Canosa, Erica Silvestris, Andrea Roberto Carosso

и другие.

Obstetrical & Gynecological Survey, Год журнала: 2025, Номер 80(2), С. 112 - 120

Опубликована: Фев. 1, 2025

Ovarian stem cells (OSCs) represent a promising tool in reproductive medicine, particularly for the treatment of premature ovarian failure and fertility preservation. Herein, we summarize main characteristics adult cells, their status, needs, new challenges application medicine. Clinical studies have shown that OSCs transplantation can restore function stimulate neo-folliculogenesis patients with failure, enabling them to conceive naturally or through vitro fertilization techniques. Moreover, gained increasing interest as chance preserve cancer undergoing gonadotoxic treatments affecting fertility, chemotherapy radiotherapy. The recruitment from fresh thawed fragments coupled capability differentiate mature oocytes could provide novel opportunity verify suitability be expanded oocyte like cells. Research into applications medicine is still its infancy, but results so far are offer possibilities suffering cancer.

Язык: Английский

Процитировано

0

The explosive discovery of TNT in early mouse embryos DOI

Chad S Driscoll,

Jae-Hwan Kim, Jason G. Knott

и другие.

Nature Structural & Molecular Biology, Год журнала: 2024, Номер 31(6), С. 852 - 855

Опубликована: Май 24, 2024

Язык: Английский

Процитировано

1

Embryoids, models, embryos? We need to take a new look at legal norms concerning the beginning of organismic development DOI Open Access
Hans‐Werner Denker

Molecular Human Reproduction, Год журнала: 2023, Номер 30(1)

Опубликована: Дек. 19, 2023

Язык: Английский

Процитировано

1

Chromatin structure in totipotent mouse early preimplantation embryos DOI Creative Commons

Masatoshi Ooga

Journal of Reproduction and Development, Год журнала: 2024, Номер 70(3), С. 152 - 159

Опубликована: Янв. 1, 2024

Totipotency refers to the ability of a single cell give rise all different types in body. Terminally differentiated germ cells (spermatozoa and oocytes) undergo reprogramming, which results acquisition totipotency zygotes. Since 1990s, numerous studies have focused on mechanisms totipotency. With emergence concept epigenetic is important for undifferentiated states cells, epigenomes fertilized eggs been thoroughly analyzed. However, early immunostaining studies, detailed epigenomic information was difficult obtain. In recent years, explosive development next-generation sequencing has made it possible acquire genome-wide genome editing facilitated analysis knockout mice, previously difficult. addition, live imaging can effectively analyze zygotes 2-cell embryos, number samples limited, provides biological insights that cannot be obtained by other methods. this review, progress our research using these advanced techniques traced back from present its earliest years.

Язык: Английский

Процитировано

0

Use of induced pluripotent stem cells for regenerative medicine and understanding of cell biology DOI Creative Commons
Kaiana Recchia, Laís Vicari de Figueirêdo Pessôa, Naira Caroline Godoy Pieri

и другие.

Reproduction Fertility and Development, Год журнала: 2024, Номер 37(1)

Опубликована: Дек. 12, 2024

Stem cells are a highly desirable tool for regenerative medicine due to unique characteristics such as immunomodulation and angiogenesis (multipotent cells) high self-renewal potential differentiation capability (pluripotent cells), thus being classified according their stage of dedifferentiation epigenetic profile. Apart from used in vitro disease modeling or even vivo therapies, pluripotent stem valuable animal production breeding improvement. In particular, the lack robustness ethical concerns regarding embryonic cells, induced (iPSCs) emerge new ‘game changer’ veterinary translational medicine. Herein, we present discuss recent uses understanding cell biology, focusing on generating using iPSCs diverse species aiming genetic conservation dissemination gametogenesis, cellular agriculture.

Язык: Английский

Процитировано

0

Stem Cell-Derived Organoids, Embryoids, and Embryos: Advances in Organismic Development In Vitro Force Us to Re-Focus on Ethical and Legal Aspects of Model Choice DOI Creative Commons
Hans‐Werner Denker

Organoids, Год журнала: 2023, Номер 2(4), С. 231 - 238

Опубликована: Дек. 5, 2023

While research on stem cell-derived tissues and organoids is rapidly expanding, the technically related creation of complex embryoids has recently excited a vivid discussion since it raises ethical questions about individuation possible gain viability. The present study focuses onset organismic development proposed biological legal definitions for terms embryo, embryoid, organoid. It concluded that such considerations have become important investigators’ choices appropriate in vitro model systems, allowing formation vs. embryoids.

Язык: Английский

Процитировано

0