Mitochondrial Oxidative Phosphorylation Alterations in Placental Tissues from Early- and Late-Onset Preeclampsia DOI Open Access

Theresa Lehenauer,

Heidi Jaksch‐Bogensperger, Sara Huber

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(9), С. 3951 - 3951

Опубликована: Апрель 22, 2025

Preeclampsia (PE), a pregnancy complication characterized by high blood pressure and organ damage, has been suggested to be associated with mitochondrial dysfunction, although evidence remains limited. This study aimed investigate the activity of oxidative phosphorylation (OXPHOS) enzymes expression related proteins in placental tissues from women diagnosed early-onset preeclampsia (eoPE, <34 weeks gestation), late-onset (loPE, ≥34 normotensive controls. Placental samples were analyzed using immunohistochemistry, western blotting, enzymatic assays assess OXPHOS complexes. Complex I was increased 80% eoPE 56% loPE, positive correlations between normalized complex expression, gestational age at delivery (r = 0.85, p 0.01), birth weight 0.88, 0.004) loPE. Relative II loPE showed duration 0.76, 0.03) 0.77, 0.03), while controls, correlated 0.64, 0.03). Additionally, IV enzyme negatively maternal −0.69, The observed highlight metabolism as promising biomarker for predicting disease progression guiding therapeutic interventions preeclampsia. Unraveling its precise role PE pathogenesis is critical advancing diagnostic precision improving maternal-fetal outcomes.

Язык: Английский

Metformin ameliorates Gestational Diabetes Mellitus via inhibiting ferroptosis of trophoblasts through the Nrf2/HO-1 signaling pathway DOI

Lingya Fang,

Sha Lu,

Liuyuan Fang

и другие.

Free Radical Research, Год журнала: 2025, Номер unknown, С. 1 - 17

Опубликована: Фев. 17, 2025

Both mothers and infants experience oxidative stress due to gestational diabetes mellitus (GDM), which is strongly associated with adverse pregnancy outcomes. Ferroptosis, a novel form of programmed cell death characterized by iron-dependent lipid peroxidation, believed play critical role in the pathogenesis progression GDM. Metformin (MET) has shown potential alleviating stress; however, research on its specific mechanisms action GDM remains limited. We collected placental tissues from patients healthy controls established an vitro model. measured markers ferroptosis including malondialdehyde (MDA), glutathione (GSH), peroxidase 4 (GPX4) activity. Additionally, we evaluated reactive oxygen species (ROS) levels, apoptosis, viability, migration Our findings revealed significant changes group compared controls, increased MDA GSSG decreased GSH reduced expression GPX4 protein placenta. High-glucose (HG) conditions were reduce trophoblast viability migration, accompanied elevated ROS as well GSH, GPX4, Nrf2, HO-1 proteins. Importantly, treatment MET reversed these effects, similar deferoxamine mesylate (DFOM), known inhibitor. These results confirm occurrence placentas demonstrate that mitigates high-glucose-induced trophoblasts through Nrf2/HO-1 signaling pathway. This study provides insights into protective MET, offering therapeutic strategies for management.

Язык: Английский

Процитировано

0
Mitochondrial Oxidative Phosphorylation Alterations in Placental Tissues from Early- and Late-Onset Preeclampsia DOI Open Access

Theresa Lehenauer,

Heidi Jaksch‐Bogensperger, Sara Huber

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(9), С. 3951 - 3951

Опубликована: Апрель 22, 2025

Preeclampsia (PE), a pregnancy complication characterized by high blood pressure and organ damage, has been suggested to be associated with mitochondrial dysfunction, although evidence remains limited. This study aimed investigate the activity of oxidative phosphorylation (OXPHOS) enzymes expression related proteins in placental tissues from women diagnosed early-onset preeclampsia (eoPE, <34 weeks gestation), late-onset (loPE, ≥34 normotensive controls. Placental samples were analyzed using immunohistochemistry, western blotting, enzymatic assays assess OXPHOS complexes. Complex I was increased 80% eoPE 56% loPE, positive correlations between normalized complex expression, gestational age at delivery (r = 0.85, p 0.01), birth weight 0.88, 0.004) loPE. Relative II loPE showed duration 0.76, 0.03) 0.77, 0.03), while controls, correlated 0.64, 0.03). Additionally, IV enzyme negatively maternal −0.69, The observed highlight metabolism as promising biomarker for predicting disease progression guiding therapeutic interventions preeclampsia. Unraveling its precise role PE pathogenesis is critical advancing diagnostic precision improving maternal-fetal outcomes.

Язык: Английский

Процитировано

0