
Cell organoid (Print), Год журнала: 2024, Номер unknown
Опубликована: Дек. 1, 2024
Patient-derived organoids (PDOs) are revolutionizing cancer research, serving as invaluable models for tumor biology and therapeutic screening. The fidelity applicability of these fundamentally shaped by the tissue dissociation techniques employed, namely mechanical enzymatic digestion. This comprehensive review delves into nuances two methods, scrutinizing their effects on solid organoid properties, including stemness, heterogeneity, long-term culturing. We discuss advantages limitations each technique, with a focus impact microenvironment preservation, application in drug screening modeling. Moreover, we examine how recent technological breakthroughs have bolstered efficiency scalability production through methods. Our analysis is designed to assist researchers choosing optimal strategy research objectives fuel evolution organoid-based models.
Язык: Английский