The pros and cons of mechanical dissociation and enzymatic digestion in patient-derived organoid cultures for solid tumor DOI Creative Commons

Jing Ren,

Mengli Liu,

Mingjie Rong

и другие.

Cell organoid (Print), Год журнала: 2024, Номер unknown

Опубликована: Дек. 1, 2024

Patient-derived organoids (PDOs) are revolutionizing cancer research, serving as invaluable models for tumor biology and therapeutic screening. The fidelity applicability of these fundamentally shaped by the tissue dissociation techniques employed, namely mechanical enzymatic digestion. This comprehensive review delves into nuances two methods, scrutinizing their effects on solid organoid properties, including stemness, heterogeneity, long-term culturing. We discuss advantages limitations each technique, with a focus impact microenvironment preservation, application in drug screening modeling. Moreover, we examine how recent technological breakthroughs have bolstered efficiency scalability production through methods. Our analysis is designed to assist researchers choosing optimal strategy research objectives fuel evolution organoid-based models.

Язык: Английский

Patient‐derived ovarian cancer organoid carries immune microenvironment and blood vessel keeping high response to cisplatin DOI Creative Commons
Yuqing Zhao, Chen Wang, Wei Deng

и другие.

MedComm, Год журнала: 2024, Номер 5(9)

Опубликована: Авг. 28, 2024

Ovarian cancer is high recurrence and mortality malignant tumor. The most common ovarian was High-Grade Serous Cancer. However, Cancer organoid rare, which with patient immune microenvironment blood vessels even absence. Here, we report a novel system derived from samples. These organoids recapitulate organoids' histological molecular heterogeneity while preserving the critical vessels, as evidenced by presence of

Язык: Английский

Процитировано

6

Advancing precision medicine: strategies from organoids research DOI
Chenzhong Li,

Qionglin Liang,

Zhong Li

и другие.

Cell organoid (Print), Год журнала: 2025, Номер unknown

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0

The pros and cons of mechanical dissociation and enzymatic digestion in patient-derived organoid cultures for solid tumor DOI Creative Commons

Jing Ren,

Mengli Liu,

Mingjie Rong

и другие.

Cell organoid (Print), Год журнала: 2024, Номер unknown

Опубликована: Дек. 1, 2024

Patient-derived organoids (PDOs) are revolutionizing cancer research, serving as invaluable models for tumor biology and therapeutic screening. The fidelity applicability of these fundamentally shaped by the tissue dissociation techniques employed, namely mechanical enzymatic digestion. This comprehensive review delves into nuances two methods, scrutinizing their effects on solid organoid properties, including stemness, heterogeneity, long-term culturing. We discuss advantages limitations each technique, with a focus impact microenvironment preservation, application in drug screening modeling. Moreover, we examine how recent technological breakthroughs have bolstered efficiency scalability production through methods. Our analysis is designed to assist researchers choosing optimal strategy research objectives fuel evolution organoid-based models.

Язык: Английский

Процитировано

0