Active induction: a promising strategy for enhancing the bioactivity of lactic acid bacteria

Critical Reviews in Food Science and Nutrition, Год журнала: 2025, Номер unknown, С. 1 - 16

Опубликована: Март 20, 2025

Lactic acid bacteria (LAB), as key probiotic, play crucial roles in maintaining human health. However, their survival and functionality diverse habitats depend on ability to sense respond environmental stresses. Notably, active induction has emerged a promising strategy for regulating the biological activity of LAB, potentially enhancing health benefits. Therefore, this review summarizes beneficial effects induction, including acid, bile, oxidation, ethanol, heat, cold, radiation functional activities LAB. In addition, omics methods, silico analysis, gene editing technologies have greatly facilitated profound exploration stress regulatory network thereby aiding identification components adaptors. Through these advancements, LAB provide benefits by stress-related genes proteins, well inducing bioactive metabolite production. As result, they could enhance tolerance, cross-protection, intestinal colonization, adhesion properties, antialcohol liver protection vitro or vivo. This study highlights potential strategies role food applications.

Язык: Английский

Bioinformatics Analysis of Diadenylate Cyclase Regulation on Cyclic Diadenosine Monophosphate Biosynthesis in Exopolysaccharide Production by Leuconostoc mesenteroides DRP105

Fermentation, Год журнала: 2025, Номер 11(4), С. 196 - 196

Опубликована: Апрель 7, 2025

Lactic acid bacteria exopolysaccharides (EPS) have a variety of excellent biological functions and are widely used in the food pharmaceutical industries. The complex metabolic system lactic mechanism EPS biosynthesis not been fully analyzed, which limits wider application EPS. synthesis is regulated by cyclic diadenosine monophosphate (c-di-AMP), but exact remains unclear. Dac pde c-di-AMP anabolic genes, gtfA, gtfB gtfC gene clusters, among was key for Leuconostoc mesenteroides DRP105. In order to explore whether diadenylate cyclase (DAC) can catalyze from ATP, sequence DAC analyzed bioinformatics based on whole genome sequence. CdaA type containing classical domain DisA_N DGA RHR motifs. secondary structure mainly composed α-helices, AlphaFold2 model 3D protein evaluate rationality model. A total 8 salt bridges, 21 hydrogen bonds 221 non-bonded interactions were found between GtfC. Molecular docking simulations revealed ATP1 ATP2 occupied binding pocket interacted directly with site residues DAC. molecular dynamics showed that ATP molecules relatively stable. Gene enzyme correlation analysis dac expression significantly positively correlated activity, content production, had no significant PDE activity responsible degradation. Bioinformatics regulatory role helpful reveal biosynthetic provide theoretical basis large-scale industrial production

Язык: Английский

Chemical Composition, Antioxidant Activity, α-Glucosidase Inhibitory Activity, and Metabolomic Profiling of Dendrobium Officinale across Diverse Growing Regions and Cultivation Modes

Food Bioscience, Год журнала: 2025, Номер unknown, С. 106714 - 106714

Опубликована: Апрель 1, 2025

Язык: Английский