人工智能定量相位成像：从物理到算法再到物理（内封面文章·特邀）

Infrared and Laser Engineering, Год журнала: 2025, Номер 54(2), С. 20240490 - 20240490

Опубликована: Янв. 1, 2025

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Nanoscale imaging of biological systems via expansion and super-resolution microscopy

Applied Physics Reviews, Год журнала: 2025, Номер 12(2)

Опубликована: Апрель 25, 2025

Super-resolution microscopy (SRM) has revolutionized life sciences by overcoming the diffraction limit, enabling visualization of biological structures at nanoscale. Expansion Microscopy (ExM) emerged as a powerful and accessible technique that enhances resolution physically enlarging specimen. Importantly, principles ExM provide unique foundation for combinations with SRM methods, pushing boundaries achievable resolution. This review explores fundamental examines its successful integration various techniques, including fluorescence fluctuation-based SRM, structured illumination microscopy, stimulated emission depletion single-molecule localization microscopy. We discuss applications, strengths, limitations, resolutions achieved these combined approaches, providing comprehensive guide researchers to select most suitable method their specific scientific needs. Key considerations when combining include impact on fluorophores, requirement specialized buffers, challenges posed sensitivity expanded hydrogels temperature hydration. Strategies address challenges, such optimized labeling techniques gel re-embedding, are discussed in detail. aims assist navigating rapidly evolving landscape facilitating development tailored imaging pipelines advance our understanding systems

Язык: Английский

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A Review of Wavefront Sensing and Control Based on Data-Driven Methods

Aerospace, Год журнала: 2025, Номер 12(5), С. 399 - 399

Опубликована: Апрель 30, 2025

Optical systems suffer from wavefront aberrations due to complex atmospheric environments and system component errors, leading systematic significantly degrading optical field quality. Therefore, the detection correction of are crucial for efficient accurate observations. To fully utilize capabilities observation equipment achieve high-efficiency, imaging, it is essential develop technologies that enable ultra-precise control. The application data-driven techniques in can effectively enhance performance better address environmental challenges. This paper elaborates on research progress methods three aspects: principles, current status, practical applications. It analyzes diverse real-world scenarios discusses future trends deep integration approaches with technologies. work provides valuable guidance advancing methodologies.

Язык: Английский

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Pixel super-resolution using compressive sensing for interferometric quantitative phase imaging

Optics and Lasers in Engineering, Год журнала: 2025, Номер 193, С. 109045 - 109045

Опубликована: Май 5, 2025

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