Optics and Lasers in Engineering, Год журнала: 2025, Номер 193, С. 109045 - 109045
Опубликована: Май 5, 2025
Optics and Lasers in Engineering, Год журнала: 2025, Номер 193, С. 109045 - 109045
Опубликована: Май 5, 2025
Infrared and Laser Engineering, Год журнала: 2025, Номер 54(2), С. 20240490 - 20240490
Опубликована: Янв. 1, 2025
Процитировано
0Applied Physics Reviews, Год журнала: 2025, Номер 12(2)
Опубликована: Апрель 25, 2025
Super-resolution microscopy (SRM) has revolutionized life sciences by overcoming the diffraction limit, enabling visualization of biological structures at nanoscale. Expansion Microscopy (ExM) emerged as a powerful and accessible technique that enhances resolution physically enlarging specimen. Importantly, principles ExM provide unique foundation for combinations with SRM methods, pushing boundaries achievable resolution. This review explores fundamental examines its successful integration various techniques, including fluorescence fluctuation-based SRM, structured illumination microscopy, stimulated emission depletion single-molecule localization microscopy. We discuss applications, strengths, limitations, resolutions achieved these combined approaches, providing comprehensive guide researchers to select most suitable method their specific scientific needs. Key considerations when combining include impact on fluorophores, requirement specialized buffers, challenges posed sensitivity expanded hydrogels temperature hydration. Strategies address challenges, such optimized labeling techniques gel re-embedding, are discussed in detail. aims assist navigating rapidly evolving landscape facilitating development tailored imaging pipelines advance our understanding systems
Язык: Английский
Процитировано
0Aerospace, Год журнала: 2025, Номер 12(5), С. 399 - 399
Опубликована: Апрель 30, 2025
Optical systems suffer from wavefront aberrations due to complex atmospheric environments and system component errors, leading systematic significantly degrading optical field quality. Therefore, the detection correction of are crucial for efficient accurate observations. To fully utilize capabilities observation equipment achieve high-efficiency, imaging, it is essential develop technologies that enable ultra-precise control. The application data-driven techniques in can effectively enhance performance better address environmental challenges. This paper elaborates on research progress methods three aspects: principles, current status, practical applications. It analyzes diverse real-world scenarios discusses future trends deep integration approaches with technologies. work provides valuable guidance advancing methodologies.
Язык: Английский
Процитировано
0Optics and Lasers in Engineering, Год журнала: 2025, Номер 193, С. 109045 - 109045
Опубликована: Май 5, 2025
Процитировано
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