Biomaterials Advances, Год журнала: 2024, Номер 166, С. 214052 - 214052
Опубликована: Сен. 25, 2024
Язык: Английский
International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(5), С. 1926 - 1926
Опубликована: Фев. 23, 2025
Various types of wounds represent a persistent healthcare burden that demands innovative and effective therapeutic solutions. Innovative approaches have emerged focus on skin regeneration with minimal side effects. One such method is cell-free therapy utilizes the secretome human mesenchymal stem cells (hMSCs) as promising alternative to traditional cell-based therapies, leveraging complex mixture bioactive molecules, including growth factors, cytokines, extracellular vesicles, accelerate tissue regeneration. This systematic review synthesizes findings 35 studies evaluating impact hMSC-derived secretomes wound healing, their regenerative, immunomodulatory, angiogenic The influence MSC sources (adipose tissue, bone marrow, umbilical cord) culture conditions composition efficacy in cutaneous healing process examined, highlighting potential regenerative medicine. also explores emerging preclinical clinical applications, results, enhanced fibroblast proliferation, reduced inflammation, improved matrix remodeling. In addition, advances secretome-based biomaterials, hydrogels scaffolds, which optimize delivery are discussed. Despite growing body evidence supporting safety secretomes, challenges remain regarding standardization, large-scale production, validation. highlights MSC-derived next-generation approach for
Язык: Английский
Процитировано
0
Biomolecules, Год журнала: 2025, Номер 15(3), С. 345 - 345
Опубликована: Фев. 27, 2025
Mesenchymal stem cells (MSCs) hold significant potential in regenerative medicine, tissue engineering, and cultivated meat production. However, large-scale MSC production is limited by their need for surface adherence during growth. This study evaluates two biocompatible materials—collagen-coated alginate microcarriers polylactic-co-glycolic acid (PLGA) nanofibers—as novel growth substrates to enhance proliferation. Physicochemical characterization confirmed successful collagen integration on both materials. In vitro, bone marrow-derived MSCs (bmMSCs) cultured collagen-coated exhibited significantly enhanced compared commercial microcarriers, while PLGA nanofibers supported bmMSC comparable traditional surfaces. Scanning Electron Microscopy (SEM) revealed that bmMSCs adhered not only the but also grew within porous structure of microcarriers. Mycoplasma testing were free from contamination. Both materials assessed biocompatibility using ISO-10993 guidelines, demonstrating no skin or ocular irritation, supporting situ applications clinical therapeutic settings. highlights promise scalable production, offering efficient, alternatives surfaces medicine manufacturing. Future research should focus optimizing these larger-scale exploring specific food sectors.
Язык: Английский
Процитировано
0
Biomolecules, Год журнала: 2025, Номер 15(3), С. 371 - 371
Опубликована: Март 4, 2025
Cardiovascular diseases are a leading cause of morbidity and mortality in dogs, with limited options available for reversing myocardial damage. Stem cell therapies have shown significant potential cardiac repair, owing to their immunomodulatory, antifibrotic, regenerative properties. This review evaluates the therapeutic applications mesenchymal stem cells (MSCs) derived from bone marrow, adipose tissue, Wharton’s jelly focus on role canine cardiology immunoregulatory Preclinical studies highlighted efficacy enhancing function, reducing fibrosis, promoting angiogenesis. Various delivery methods, including intracoronary intramyocardial injections, assessed safety efficacy. Challenges such as low retention, differentiation efficiency, variability responses also discussed. Emerging strategies, genetic modifications combination therapies, aim enhance MSCs. Additionally, advances systems regulatory frameworks reviewed support clinical translation. comprehensive evaluation underscores revolutionize cardiovascular disease management while identifying critical areas future research integration.
Язык: Английский
Процитировано
0
International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(3), С. 1311 - 1311
Опубликована: Фев. 4, 2025
Mesenchymal stem cells (MSCs) are crucial for skeletal development, homeostasis, and repair, primarily through their differentiation into osteoblasts other lineage cells. Key signaling pathways, including Wnt, TGF-β/BMP, PTH, Hedgehog, IGF, act as critical regulators of MSC osteogenesis, playing pivotal roles in maintaining bone homeostasis facilitating regeneration. These pathways interact distinct ways at various stages mineralization, remodeling. This review provides an overview the molecular mechanisms by which these regulate influence on tissue formation, implications diseases therapeutic strategies. Additionally, we explore potential applications engineering, with a particular focus promoting use MSCs seed defect repair. Ultimately, this aims to highlight avenues advancing biology research, treating disorders, enhancing regenerative medicine.
Язык: Английский
Процитировано
0
Journal of Functional Biomaterials, Год журнала: 2025, Номер 16(3), С. 97 - 97
Опубликована: Март 11, 2025
Zone II flexor digitorum profundus (FDP) tendon injuries are complex, and present significant challenges in hand surgery, due to the need balance strength flexibility during repair. Traditional suture techniques often lead complications such as adhesions or rupture, prompting exploration of novel strategies improve outcomes. This review investigates use superficialis (FDS) autografts reinforce FDP repairs, alongside integration biomaterials enhance mechanical without sacrificing FDS tissue. Key biomaterials, including collagen–polycaprolactone (PCL) composites, evaluated for their biocompatibility, integrity, controlled degradation properties. Collagen-PCL emerges a leading candidate, offering potential reduce promote healing. Although nanomaterials nanofibers nanoparticles show promise preventing supporting cellular proliferation, application remains limited by manufacturing challenges. By combining advanced repair with like collagen-PCL, this approach aims surgical outcomes minimize complications. Future research will focus on validating these findings biological models, assessing healing through imaging, comparing cost-effectiveness biomaterial-enhanced repairs traditional methods. underscores biomaterial-based approaches transform
Язык: Английский
Процитировано
0
Gels, Год журнала: 2025, Номер 11(4), С. 250 - 250
Опубликована: Март 27, 2025
Cirrhosis, a marker of severe liver diseases, limits future remnant (FLR) growth, preventing many cancer patients from undergoing surgery. While portal vein blockade (PVB) techniques are used to stimulate regeneration, 20–30% still fail achieve the required growth. Although mesenchymal stem cell (MSC) therapy improves PVB, its efficacy is limited by poor retention. To address this, we utilized alginate hydrogels deliver MSCs and improve their were loaded in hydrogel injected intraportally cirrhotic rats. Liver volume, weights, enzyme levels, histology monitored. Results showed that maintained 89.0 ± 3.0% viability gradually released for over two weeks. Furthermore, rats with MSC-loaded demonstrated higher volumes (FLR ratio 0.57 0.32) weights 0.84 0.05). The treated exhibited more improved enzymes (AST: 72.75 14.17 U/L, ALP: 135.67 41.20 ALT: 46.00 2.94 U/L) decreased fibrotic areas (4.52 0.22%) compared control group. Histology revealed increased retention when delivered (37.30 16.10 MSCs/mm2) cells alone (21.70 22.10 MSCs/mm2). Overall, enhanced growth reduced fibrosis promoting This approach holds significant potential improving outcomes among patients, offering promising therapeutic strategy regeneration treatment diseases.
Язык: Английский
Процитировано
0
Biomaterials Advances, Год журнала: 2024, Номер 166, С. 214052 - 214052
Опубликована: Сен. 25, 2024
Язык: Английский
Процитировано
1