Role of vimentin in the morphogenesis of endometrial decidualization during pregnancy with IVF methods
Journal of obstetrics and women s diseases,
Год журнала:
2024,
Номер
73(3), С. 81 - 88
Опубликована: Июль 17, 2024
BACKGROUND:
Early
reproductive
loss
is
a
complex
problem,
the
solution
of
which
requires
systematic
approach
to
study
morphogenesis
gravid
transformation.
Violations
morphofunctional
characteristics
endometrium
are
associated
with
chronic
endometritis,
entails
implantation
pathology
and
development
pregnancy,
while
formulating
indications
for
use
IVF
methods.
Chronic
endometritis
disrupts
differentiation
stromal
cells
intermediate
filaments
subsequent
decidualization
AIM:
The
aim
this
was
evaluate
vimentin
expression
in
decidual
an
undeveloped
first-trimester
pregnancy
after
IVF,
depending
on
its
transformation
patients
endometritis.
MATERIALS
AND
METHODS:
Histological
immunohistochemical
studies
were
performed
40
samples
abortion
material
normal
chorionic
villi
karyotype
from
non-developing
15
progressive
surgically
terminated
at
woman’s
request
similar
terms.
Two
groups
formed
—
I
group
full-fledged
(n
=
20)
II
incomplete
20).
examination
according
standard
procedure.
Immunohistochemical
using
one-step
protocol
antigen
unmasking.
Primary
monoclonal
antibodies
(Clon
V9,
Diagnostic
BioSystems
Inc.,
Spain)
used
1
:
1000
dilution.
Vimentin
evaluated
compact
layer
endometrium.
RESULTS:
A
endometrial
stroma
control
represented
by
mature
deciduocytes
epithelioid
type,
round
or
polygonal
shape,
light
vesicular
nucleus.
With
transformation,
fibroblasts
varying
degrees
cytoplasmic
development,
mild
changes
formation
pre-decidual
immature
cells.
decrease
verified
compared
group.
In
case
also
I.
CONCLUSIONS:
presence
infertility
not
only
causes
original
endometrium,
but
decidualization,
leading
embryo
karyotype.
Язык: Английский
COMPARATIVE ANALYSIS OF APOPTOSIS AND HYPOXIA IN PLACENTAS OF PATIENTS WITH COVID-19 AND CRITICAL LUNG LESION (IMMUNOHISTOCHEMICAL EXAMINATION)
Laboratornaya i klinicheskaya meditsina Farmatsiya,
Год журнала:
2024,
Номер
12, С. 25 - 34
Опубликована: Янв. 1, 2024
Relevance.
The
risk
of
developing
severe
stage
COVID-19
infection
in
pregnant
women
is
quite
high.
study
apoptosis
and
hypoxia
placenta
which
have
a
fundamental
impact
on
functioning
this
organ
lung
lesion
varying
severity
important
for
the
justification
obstetric
tactics.
aim
was
to
determine
expression
level
apoptosis-inducing
factor
(AIF)
hypoxia-inducible
(HIF-1?)
placentas
with
critical
comparative
aspect.
Material
methods.
AIF
HIF-1?
studied.
1-st
group
consisted
25
(CLL)
(more
than
75
%,
CT-4)
after
preterm
labor
induced
by
mother's
condition,
every
third
case
combination
fetal
distress;
2-nd
patients
–
50%
(CT-2)
spontaneous
at
clinical
recovery
COVID-19.
Results.
median
area
immunopositive
structures
respect
statistically
significantly
higher
2
amounted
66.38
(60.79;
74.51)
%
vs.
56.11
(28.04;
63.18)
(p
=
0.000).
against
17.80
(13.33;
27.33)
33.01
(27.63;
35.99)
respectively
groups
Conclusion.
In
CLL,
same
parameter
0.000),
may
indicate
energy
deficit
cyto-
syncytiotrophoblast
cells
due
condition
CLL.
On
contrary,
1
lower
be
delivery
CLL
before
development
hypoxic
damage
placentas.
Язык: Английский