Snowball: a novel gene family required for developmental patterning of fruiting bodies of mushroom-forming fungi (Agaricomycetes)

mSystems, Год журнала: 2024, Номер 9(3)

Опубликована: Фев. 9, 2024

The morphogenesis of sexual fruiting bodies fungi is a complex process determined by genetically encoded program. Fruiting reached the highest complexity levels in Agaricomycetes; yet, underlying genetics currently poorly known. In this work, we functionally characterized highly conserved gene termed

Язык: Английский

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Sexual spores in mushrooms: bioactive compounds, factors and molecular mechanisms of spore formation

Archives of Microbiology, Год журнала: 2025, Номер 207(2)

Опубликована: Янв. 21, 2025

Язык: Английский

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Shedding light on Pleurotus: an update on taxonomy, properties, and photobiology

Microbiological Research, Год журнала: 2025, Номер 295, С. 128110 - 128110

Опубликована: Фев. 20, 2025

Язык: Английский

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Cultivation methods and biology of Lentinula edodes

Applied Microbiology and Biotechnology, Год журнала: 2025, Номер 109(1)

Опубликована: Март 11, 2025

In this study, the biological applications of cultivation methods related to cultivar selection, vegetative growth, and reproductive development in Lentinula edodes are briefly reviewed clarify current situation inform future developments. The cultivars widely used main production areas derived from wild strains distributed northern Asia. most effective techniques for identification molecular markers identified two nuclear genome datasets one mitochondrial dataset. stage breeding is at junction Breeding 3.0 (biological breeding) 4.0 (intelligent breeding). Plant breeder's rights patents have different emphases on new variety protection, with former being utilized globally. L. mostly produced synthetic logs filled sawdust substrates. Hardwood comprises approximately 80% growth involves distinct stages mycelial colonization browning. Mycelia mainly perform glycolysis, tricarboxylic acid cycle, respiratory metabolism reactions produce energy intermediates synthesizing structural components hyphae stage. Upon stimulation by physiological environmental pressures after colonization, mycelia trigger gluconeogenesis, autophagy, secondary metabolism, increase metabolic flux pentose phosphate pathway, activate glyoxylate accumulate melanin surface ensure survival. Sexually competent can form hyphal knots as a result reprogrammed branching patterns period (which varies cultivar) specific factors. Under genetically encoded developmental program, undergo aggregation, tissue differentiation, primordium formation, meiosis hymenium, stipe elongation, basidiospore maturation, cap expansion mature fruiting bodies. Growers achieve good body shape high yield regulating number young bodies adjusting • Cultivar selection becomes less increasing technological requirement cultivation. showed events browning stages. Specific breading may be next milestone

Язык: Английский

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Morphogenesis, starvation, and light responses in a mushroom-forming fungus revealed by long-read sequencing and extensive expression profiling

Cell Genomics, Год журнала: 2025, Номер unknown, С. 100853 - 100853

Опубликована: Апрель 1, 2025

Mushroom-forming fungi (Agaricomycetes) are emerging as pivotal players in several fields of science and industry. Genomic data for Agaricomycetes accumulating rapidly; however, this is not paralleled by improvements gene annotations, which leave function notoriously poorly understood. We set out to improve our functional understanding the model mushroom Coprinopsis cinerea integrating a new, chromosome-level assembly, high-quality predictions, information derived from broad gene-expression profiling data. The new annotation includes 5' 3' untranslated regions (UTRs), polyadenylation sites (PASs), upstream open reading frames (uORFs), splicing isoforms, microexons, well core sets corresponding carbon starvation, light response, hyphal differentiation. As result, genome C. has now become most comprehensively annotated among mushroom-forming fungi, will contribute multiple rapidly expanding fields, including research on their life history, stress responses, multicellular development.

Язык: Английский

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Multi-omics insights into growth and fruiting body development in the entomopathogenic fungus Cordyceps blackwelliae

IMA Fungus, Год журнала: 2025, Номер 16

Опубликована: Май 7, 2025

Cordyceps blackwelliae is an entomopathogenic fungus with significant potential for research and development due to its ease of cultivation. However, the lack omics-based studies has limited our understanding molecular mechanisms governing growth fruiting body development. This study employed a multi-omics approach, integrating genomic, transcriptomic metabolomic analyses. Utilising both Illumina Nanopore sequencing technologies, we assembled 31.06 Mb nuclear genome comprising 11 scaffolds, telomere presence at one or ends in eight scaffolds annotated 8,138 identified genes (8,136 from prediction two local BLAST searches). Transcriptomic analysis 2,078 differentially expressed across three developmental stages: liquid culture mycelia, wheat mycelia bodies. Amongst these, 745 were up-regulated bodies, primarily associated biosynthetic catabolic pathways. Metabolomic 1,161 metabolites, 1,014 showing variations stages. Integrated analyses uncovered 17 positively correlated 34 which are likely crucial regulators These findings provide new insights into networks underlying C. formation.

Язык: Английский

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Unraveling Morphogenesis, Starvation, and Light Responses in a Mushroom-Forming Fungus,Coprinopsis cinerea, Using Long Read Sequencing and Extensive Expression Profiling

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Май 10, 2024

Abstract Mushroom-forming fungi (Agaricomycetes) are emerging as pivotal players in several fields, drivers of nutrient cycling, sources novel applications, and the group includes some most morphologically complex multicellular fungi. Genomic data for Agaricomycetes accumulating at a steady pace, however, this is not paralleled by improvements quality genome sequence associated functional gene annotations, which leaves function notoriously poorly understood comparison with other model eukaryotes. We set out to improve our understanding mushroom Coprinopsis cinerea integrating new, chromosome-level assembly high-quality predictions information derived from gene-expression profiling across 67 developmental, stress, light conditions. The new annotation has considerably improved metrics 5’- 3’-untranslated regions (UTRs), polyadenylation sites (PAS), upstream ORFs (uORFs), splicing isoforms, conserved motifs (e.g., TATA Kozak boxes) microexons. found that alternative widespread C. , but it specifically regulated various conditions used here. Transcriptome allowed us delineate core sets corresponding carbon starvation, light-response, hyphal differentiation, uncover aspects light-regulated phases life cycle. As result, now become comprehensively annotated among mushroom-forming fungi, will contribute multiple rapidly expanding including research on their history, stress responses, well development.

Язык: Английский

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The Role of Chromatin and Transcriptional Control in the Formation of Sexual Fruiting Bodies in Fungi

Microbiology and Molecular Biology Reviews, Год журнала: 2022, Номер 86(4)

Опубликована: Ноя. 21, 2022

Fungal fruiting bodies are complex, three-dimensional structures that arise from a less complex vegetative mycelium. Their formation requires the coordinated action of many genes and their gene products, body is accompanied by major changes in transcriptome.

Язык: Английский

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Mushroom functional genomics springs up

The Innovation Life, Год журнала: 2023, Номер 1(1), С. 100005 - 100005

Опубликована: Янв. 1, 2023

Язык: Английский

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Goldilocks mushrooms: How ballistospory has shaped basidiomycete evolution

Fungal Biology, Год журнала: 2023, Номер 127(4), С. 975 - 984

Опубликована: Март 9, 2023

Ballistospory has been a governing factor in mushroom diversification. Modifications to fruit body morphology are subject series of fundamental constraints imposed by this uniquely fungal mechanism. Gill spacing lamellate mushrooms, tube width poroid species, and other configurations the hymenium must comply with distance that spores shoot themselves from their basidia. This reciprocal relationship between development bodies may have maintained form evolutionary seesaw proposed article. The necessity accurate gravitropic orientation gills tubes is another constraint on physiology, along importance evaporative cooling for successful spore discharge aerodynamic shaping aid dispersal. lost secotioid gasteroid basidiomycetes whose dispersed animal vectors replaced alterative mechanisms active some species. Partnered conclusions drawn molecular phylogenetic research, biomechanical themes discussed review afford new ways think about evolution basidiomycetes.

Язык: Английский

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