NsdD Is a Key Repressor of Asexual Development inAspergillus nidulans DOI Open Access
Mi-Kyung Lee, Nak‐Jung Kwon, Jae Min Choi

и другие.

Genetics, Год журнала: 2014, Номер 197(1), С. 159 - 173

Опубликована: Фев. 16, 2014

Abstract Asexual development (conidiation) of the filamentous fungus Aspergillus nidulans occurs via balanced activities multiple positive and negative regulators. For instance, FluG (+) SfgA (−) govern upstream regulation developmental switch, BrlA VosA control progression completion conidiation. To identify regulators conidiation downstream FluG-SfgA, we carried out multicopy genetic screens using sfgA deletion strains. After visually screening >100,000 colonies, isolated 61 transformants exhibiting reduced Responsible genes were identified as AN3152 (nsdD), AN7507, AN2009, AN1652, AN5833, AN9141. Importantly, nsdD, a key activator sexual reproduction, was present in 10 independent transformants. Furthermore, deletion, overexpression, double-mutant analyses individual have led to conclusion that, six genes, only nsdD functions FluG-activated pathway. The bypassed need for fluG flbA∼flbE, but not brlA or abaA, conidiation, partially restored production mycotoxin sterigmatocystin (ST) ΔfluG, ΔflbA, ΔflbB mutants, suggesting that NsdD is positioned between FLBs A. nidulans. Nullifying caused formation conidiophores liquid submerged cultures, where wild-type strains do develop. Moreover, removal both vosA resulted even more abundant cultures high-level accumulation messenger (m)RNA at 16 hr vegetative growth. Collectively, regulator likely exerts its repressive role downregulating brlA.

Язык: Английский

Negative regulation and developmental competence in Aspergillus DOI Creative Commons
Mi-Kyung Lee, Nak‐Jung Kwon, Im‐Soon Lee

и другие.

Scientific Reports, Год журнала: 2016, Номер 6(1)

Опубликована: Июль 1, 2016

Abstract Asexual development (conidiation) in the filamentous fungus Aspergillus nidulans is governed by orchestrated gene expression. The three key negative regulators of conidiation SfgA, VosA, and NsdD act at different control point developmental genetic cascade. Here, we have revealed that a repressor affecting quantity asexual spores . Moreover, nullifying both nsdD vosA results abundant formation specific structure conidiophores even 12 h liquid culture, near constitutive activation conidiation, indicating acquisition competence involves removal regulation exerted VosA. NsdD’s role repressing conserved other aspergilli, as deleting causes enhanced precocious fumigatus or flavus In vivo NsdD-DNA interaction analyses identify binding regions promoter essential activator brlA , direct repressive conidiation. Importantly, loss flbC flbD encoding upstream activators absence delayed suggesting distinct positive roles FlbC FlbD A model depicting A. presented.

Язык: Английский

Процитировано

89
The ancient koji mold (Aspergillus oryzae) as a modern biotechnological tool DOI Creative Commons
Ghoson M. Daba, Faten A. Mostafa, Waill A. Elkhateeb

и другие.

Bioresources and Bioprocessing, Год журнала: 2021, Номер 8(1)

Опубликована: Июнь 22, 2021

Abstract Aspergillus oryzae ( A. ) is a filamentous micro-fungus that used from centuries in fermentation of different foods many countries all over the world. This valuable fungus also rich source bioactive secondary metabolites. Moreover, has prestigious secretory system allows it to secrete high concentrations proteins into its culturing medium, which support use as biotechnological tool veterinary, food, pharmaceutical, and industrial fields. review aims highlight significance this food industry, showing generosity production nutritional metabolites enrich fermented by it. Also, using field enzymes was described. Furthermore, domestication, functional genomics, contributions human pharmaceutical were presented. Finally, future prospects order get more benefits discussed.

Язык: Английский

Процитировано

80
Transcription in fungal conidia before dormancy produces phenotypically variable conidia that maximize survival in different environments DOI
Fang Wang, Pooja Sethiya, Xiaohui Hu

и другие.

Nature Microbiology, Год журнала: 2021, Номер 6(8), С. 1066 - 1081

Опубликована: Июнь 28, 2021

Язык: Английский

Процитировано

73
Aspergillus niger Spores Are Highly Resistant to Space Radiation DOI Creative Commons
Marta Cortesão, Aram de Haas,

Rebecca Unterbusch

и другие.

Frontiers in Microbiology, Год журнала: 2020, Номер 11

Опубликована: Апрель 3, 2020

The filamentous fungus Aspergillus niger is one of the main contaminants International Space Station (ISS). It forms highly pigmented, airborne spores that have thick cell walls and low metabolic activity, enabling them to withstand harsh conditions colonize spacecraft surfaces. Whether A. are resistant space radiation, what extent, not yet known. In this study, spore suspensions a wild-type three mutant strains (with defects in pigmentation, DNA repair polar growth control) were exposed X-rays, cosmic radiation (helium iron-ions) UV-C (254 nm). To assess level resistance survival limits fungal long-term interplanetary mission scenario, we tested doses up 1000 Gy 4000 J/m2. For comparison, 360-day round-trip Mars yields dose 0.66 ± 0.12 Gy. Overall, able high X-ray (LD90 = 360 Gy) (helium-ion LD90 500 Gy; iron-ion LD90= 100 Gy). Drying before irradiation made more susceptible towards radiation. Notably, 1038 J/m2), which significantly higher than other radiation-resistant microorganisms (e.g. Deinococcus radiodurans). all strains, treated (1000 J/m2) shown decreased biofilm formation (81 % reduction spores). This study suggests might be easily inactivated by exposure alone current planetary protection guidelines should revisited, considering spores.

Язык: Английский

Процитировано

71
Regulation of Conidiogenesis in Aspergillus flavus DOI Creative Commons

He-Jin Cho,

Sung-Hun Son,

Wanping Chen

и другие.

Cells, Год журнала: 2022, Номер 11(18), С. 2796 - 2796

Опубликована: Сен. 7, 2022

Aspergillus flavus is a representative fungal species in the section Flavi and has been used as model system to gain insights into development toxin production. A. several adverse effects on humans, including production of most carcinogenic mycotoxin aflatoxins causing aspergillosis immune-compromised patients. In addition, infection crops results economic losses due yield loss aflatoxin contamination. saprophytic fungus that disperses ecosystem mainly by producing asexual spores (conidia), which also provide long-term survival harsh environmental conditions. Conidia are composed rodlet layer, cell wall, melanin produced from an specialized structure called conidiophore. The conidiophores tightly regulated various regulators, central regulatory cascade BrlA-AbaA-WetA, fungi-specific velvet upstream developmental repressors. this review, we summarize findings series recent studies related for better understanding other Flavi.

Язык: Английский

Процитировано

50
Aerosolization of fungal spores in indoor environments DOI
Xian Li, Dan Liu,

Jian Guo Yao

и другие.

The Science of The Total Environment, Год журнала: 2022, Номер 820, С. 153003 - 153003

Опубликована: Янв. 11, 2022

Язык: Английский

Процитировано

42
Lessons on fruiting body morphogenesis from genomes and transcriptomes of Agaricomycetes DOI Creative Commons
László G. Nagy, Peter Jan Vonk, Markus Künzler

и другие.

Studies in Mycology, Год журнала: 2023, Номер 104(1), С. 1 - 85

Опубликована: Фев. 1, 2023

Язык: Английский

Процитировано

31
Occurrence of aflatoxins in water and decontamination strategies: A review DOI

Suyan Wang,

Daniela D. Herrera‐Balandrano, Xin‐Chi Shi

и другие.

Water Research, Год журнала: 2023, Номер 232, С. 119703 - 119703

Опубликована: Фев. 4, 2023

Язык: Английский

Процитировано

24
Aspergillus oryzae as a Cell Factory: Research and Applications in Industrial Production DOI Creative Commons
Zeao Sun, Yijian Wu,

Shihua Long

и другие.

Journal of Fungi, Год журнала: 2024, Номер 10(4), С. 248 - 248

Опубликована: Март 26, 2024

Aspergillus oryzae, a biosafe strain widely utilized in bioproduction and fermentation technology, exhibits robust hydrolytic enzyme secretion system. Therefore, it is frequently employed as cell factory for industrial production. Moreover, A. oryzae has the ability to synthesize various secondary metabolites, such kojic acid L-malic acid. Nevertheless, complex system protein expression regulation mechanism of pose challenges expressing numerous heterologous products. By leveraging synthetic biology novel genetic engineering techniques, emerged an ideal candidate constructing factories. In this review, we provide overview latest advancements application oryzae-based factories These studies suggest that metabolic optimization are key elements realizing widespread It anticipated review will pave way more effective approaches research avenues future implementation

Язык: Английский

Процитировано

15
Interplay of Cytokines and Chemokines in Aspergillosis DOI Creative Commons
Jata Shankar, Raman Thakur, Karl V. Clemons

и другие.

Journal of Fungi, Год журнала: 2024, Номер 10(4), С. 251 - 251

Опубликована: Март 27, 2024

Aspergillosis is a fungal infection caused by various species of Aspergillus, most notably A. fumigatus. This fungus causes spectrum diseases, including allergic bronchopulmonary aspergillosis, aspergilloma, chronic pulmonary and invasive aspergillosis. The clinical manifestations severity aspergillosis can vary depending on individual immune status the specific Aspergillus involved. recognition involves pathogen-associated molecular patterns (PAMPs) such as glucan, galactomannan, mannose, conidial surface proteins. These are recognized pathogen receptors present cells Toll-like (TLR-1,2,3,4, etc.) C-type lectins (Dectin-1 Dectin-2). We discuss roles cytokines in from both perspective human experimental infection. Several chemokines have been implicated response to infection, interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α), CCR4, CCR17, other interleukins. For example, (ABPA) characterized Th2 Th9 cell-type immunity interleukin (IL)-4, IL-5, IL-13, IL-10. In contrast, it has observed that Th1 Th17 via IFN-γ, IL-1, IL-6, IL-17. activate stimulate production molecules, antimicrobial peptides reactive oxygen species, which aid clearance pathogen. Moreover, they help initiate coordinate response, recruit site promote fungus. Insight into host animal studies may understanding possibly leading harnessing power or cytokine (receptor) antagonists transforming them precise immunotherapeutic strategies. could advance personalized medicine.

Язык: Английский

Процитировано

15