Genetics, Год журнала: 2014, Номер 197(1), С. 159 - 173

Опубликована: Фев. 16, 2014

Abstract Asexual development (conidiation) of the filamentous fungus Aspergillus nidulans occurs via balanced activities multiple positive and negative regulators. For instance, FluG (+) SfgA (−) govern upstream regulation developmental switch, BrlA VosA control progression completion conidiation. To identify regulators conidiation downstream FluG-SfgA, we carried out multicopy genetic screens using sfgA deletion strains. After visually screening >100,000 colonies, isolated 61 transformants exhibiting reduced Responsible genes were identified as AN3152 (nsdD), AN7507, AN2009, AN1652, AN5833, AN9141. Importantly, nsdD, a key activator sexual reproduction, was present in 10 independent transformants. Furthermore, deletion, overexpression, double-mutant analyses individual have led to conclusion that, six genes, only nsdD functions FluG-activated pathway. The bypassed need for fluG flbA∼flbE, but not brlA or abaA, conidiation, partially restored production mycotoxin sterigmatocystin (ST) ΔfluG, ΔflbA, ΔflbB mutants, suggesting that NsdD is positioned between FLBs A. nidulans. Nullifying caused formation conidiophores liquid submerged cultures, where wild-type strains do develop. Moreover, removal both vosA resulted even more abundant cultures high-level accumulation messenger (m)RNA at 16 hr vegetative growth. Collectively, regulator likely exerts its repressive role downregulating brlA.

Язык: Английский