Animal Reproduction,
Год журнала:
2023,
Номер
20(3)
Опубликована: Янв. 1, 2023
The
aim
of
this
study
was
to
compare
embryo
production
efficiency
in
Flemish
and
Holstein
donor
females
using
ovum
pick-up
vitro
fertilization
(OPU-IVF)
or
vivo
(superovulation;
SOV)
procedures.
conducted
a
split-plot
design,
with
eight
non-lactating
cycling
females.
Females
were
subjected
ten
weekly
OPU/IVF
sessions
and/or
two
SOV/embryo
collections
at
63-day
interval,
for
total
160
OPU-IVF
32
SOV
sessions.
Mean
numbers
follicles
corpora
lutea,
cumulus-oocyte
complex
(COC)
recovery
rates
similar
between
breeds
after
the
OPU
However,
donors
yielded
better
quality
grade
II
COCs
(301,
41.9%)
than
(609,
202,
33.1%).
Also,
cleavage
blastocyst
rates,
number
mean
viable
embryos
obtained
higher
(49.6%
11.8%,
63
11.8
per
donor,
respectively)
(32.8%
7.2%,
34
7.2
also
more
efficient
yielding
(111,
7.3
donor)
(48,
3.3
Overall,
had
responses
procedures
counterparts.
Irrespective
breeds,
embryos,
under
conditions
study.
Both
reproductive
useful
tools
genetic
conservation
cattle
breed
Southern
Brazil.
Animals,
Год журнала:
2025,
Номер
15(5), С. 631 - 631
Опубликована: Фев. 21, 2025
Assisted
reproductive
technologies
(ART)
are
routinely
used
in
livestock
to
generate
animals
of
high
genetic
value.
Despite
representing
an
outstanding
accomplishment,
recent
studies
suggest
differences
health,
fertility,
and
gestational
length
vitro-produced
compared
vivo-derived
animals.
Currently,
there
no
data
available
on
the
long-term
effects
ART
growth
development.
This
observational
study
aimed
understand
relationship
between
growth-influencing
hormones
a
herd
cattle
derived
from
artificial
insemination
(AI)
or
embryos
either
with
BSA
(C-IVP)
fluids
(RF-IVP)
as
protein
source
culture.
Cortisol
was
associated
positively
weight
AI
negatively
body
males.
Thyroxine
decreased
age,
it
thoracic
circumference
RF-IVP.
Insulin-like
factor-1
greater
RF-IVP
than
C-IVP,
withers
height.
Growth
hormone
females
males
C-IVP.
In
conclusion,
we
present
here
first
datasets
parameters
birth
4
years
age
without
observing
major
evidence
depending
embryo
origin.
Biology of Reproduction,
Год журнала:
2024,
Номер
unknown
Опубликована: Июнь 8, 2024
Choline
is
a
vital
micronutrient
that
can
be
utilized
in
the
formation
of
betaine
and
multiple
phospholipids.
In
this
study,
we
aimed
to
confirm,
expand
on
previous
findings,
how
choline
impacts
embryos
from
first
7
days
development
affect
postnatal
phenotype.
Bos
indicus
were
cultured
choline-free
medium
(termed
vehicle)
or
supplemented
with
1.8
mM
Blastocyst-stage
transferred
into
crossbred
recipients.
Once
born,
calves
evaluated
at
birth,
94
d,
178
d
weaning
(average
age
=
239
d).
Following
weaning,
all
enrolled
feed
efficiency
trial
before
being
separated
by
sex,
males
slaughtered
approximately
580
females
followed
until
their
pregnancy
check.
Results
confirm
exposure
chloride
during
alters
characteristics
resultant
calves.
Calves
both
sexes
choline-treated
consistently
heavier
through
had
testes
3
mo
age.
There
sex-dependent
alterations
DNA
methylation
whole
blood
caused
treatment.
After
was
affected
an
interaction
more
efficient
for
less
males.
heavier,
tended
than
vehicle
observations
after
weaning.
Carcass
weight
cross-sectional
area
Longissumus
thoracis
muscle
increased
choline.
Few
became
pregnant
experiment
although
numerically
females.
preimplantation
embryo
alter
phenotypes
19
months
birth.
Theriogenology,
Год журнала:
2024,
Номер
225, С. 16 - 32
Опубликована: Май 20, 2024
The
challenges
posed
by
climate
change
and
increasing
world
population
are
stimulating
renewed
efforts
for
improving
the
sustainability
of
animal
production.
To
meet
such
challenges,
contribution
genomic
selection
approaches,
in
combination
with
assisted
reproductive
technologies
(ARTs),
to
spreading
preserving
genetics
is
essential.
largest
increase
genetic
gain
can
be
achieved
shortening
generation
interval.
This
review
provides
an
overview
current
status
progress
advanced
ARTs
that
could
applied
reduce
time
both
female
male
domestic
ruminants.
In
females,
use
juvenile
vitro
embryo
transfer
(JIVET)
enables
generate
offspring
after
produced
embryos
derived
from
oocytes
prepubertal
genetically
superior
donors
reducing
generational
interval
acceleration
gain.
challenge
production
(IVEP)
which
still
low
variable.
two
main
factors
limiting
IVEP
success
intrinsic
quality
culture
systems
maturation
(IVM).
males,
advancements
providing
new
strategies
propagate
spermatogonia
differentiate
them
into
mature
sperm
or
even
recapitulate
whole
process
spermatogenesis
embryonic
stem
cells.
Moreover,
successful
immature
cells,
as
round
spermatids,
intracytoplasmic
injection
(ROSI)
allow
complete
entire
few
months.
However,
these
approaches
have
been
successfully
human
mouse
whereas
only
a
studies
published
ruminants
results
controversial.
also
dependent
on
efficiency
ROSI
limited
isolation
protocols
spermatids.
conclusion,
methodologies
lead
toward
significant
reduction
livestock
animals
considerable
impact
agriculture
sustainability.
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(5), С. 1989 - 1989
Опубликована: Фев. 25, 2025
In
small
ruminants,
laparotomy
for
ovarian
exploration
followed
by
oocyte
collection
has
been
progressively
replaced
laparoscopic
puncture
of
follicles,
which
become
an
important
method
obtaining
oocytes
in
vivo.
However,
the
superovulation
protocols
and
frequency
used
ovum
pick-up
(LOPU)
sheep
still
require
further
investigation.
This
study
explored
factors
influencing
LOPU
efficiency
sheep,
including
Controlled
Internal
Drug
Release
(CIDR)
estrus
synchronization,
FSH
source
dose,
recovery
intervals.
The
optimal
protocol
(using
CIDR
device,
a
total
16
mg
long-acting
recombinant
ovine
(LR-FSH)
administered
two
doses,
one-month
interval
between
sessions)
was
subsequently
identified.
Ovarian
follicles
were
collected
via
from
Hu
Altay
transcriptomic
metabolomic
sequencing
to
explore
interbreed
differences
follicular
development.
results
indicated
that
significantly
higher
group
(p
<
0.05)
with
30-day
0.05).
No
significant
observed
sources
or
hormone
doses.
Furthermore,
exhibited
more
antral
than
sheep.
Transcriptomic
analysis
contents
profiling
fluid
revealed
differentially
expressed
genes
metabolites
primarily
enriched
pathways
related
steroidogenesis,
amino
acid
metabolism,
fatty
metabolism.
provides
optimized
treatment
enhance
integrates
multi-omics
analyses
elucidate
molecular
mechanisms
underlying
development
among
various
breeds.
Journal of Dairy Science,
Год журнала:
2024,
Номер
107(8), С. 5913 - 5923
Опубликована: Апрель 11, 2024
The
performance
of
an
adult
dairy
cow
may
be
influenced
by
events
that
occur
before
her
birth.
present
study
investigated
potential
effects
2
prenatal
groups
factors,
Assisted
Reproductive
Techniques
(ART)
and
maternal
characteristics
(e.g.,
dam
parity),
on
offspring
during
their
first
lactation,
in
populations
breeds:
French
Holstein
Montbéliarde.
different
ART
studied
included
the
type
semen
(conventional
or
X-sorted)
used
for
Artificial
Insemination
(AI)
technology
conception
(AI,
embryo
transfer,
vitro
fertilization).
Three
were
considered:
(1)
age
at
calving,
(2)
parity
number,
(3)
indicators
udder
health
gestation
(somatic
cell
score
clinical
mastitis).
First,
we
whether
heifer
survival
from
3d
to
18
mo
old
was
associated
with
any
factors
considered.
We
then
estimated
associations
these
8
traits
commercial
interest:
stature,
(2–4)
milk,
fat,
protein
yields,
(5)
somatic
score,
(6)
mastitis,
(7–8)
rate,
all
measured
genotyped
cows.
Linear
models
this
as
covariates
model,
traits,
phenotypes
adjusted
corresponding
genomic
breeding
value.
results
indicated
rate
heifers
born
transfer
significantly
higher
than
AI
(probably
due
preferential
management
practices),
while
other
did
not
explain
differences
survival.
Among
Montbéliarde
cows
AI,
those
X-sorted
showed
a
lightly
but
lower
milk
yield
without
X-sorting
(−52
kg
lactation).
cows,
presented
AI.
Regarding
characteristics,
none
very
weak
found
between
calving
both
breeds.
Dam
parity,
hand,
breeds,
however
same
direction.
In
breed,
increase
favorable
yield,
whereas
no
association
fat
yield.
only
weakly
offspring.
Although
some
significant
identified
large
sample
size,
modest,
typically
less
1%
phenotypic
mean,
consistently
observed
across
Animal Reproduction,
Год журнала:
2024,
Номер
21(3)
Опубликована: Янв. 1, 2024
For
nearly
100
years
the
postcoital
inflammatory
response
has
been
described
in
female
reproductive
tract
of
rodents.
Since
1950's
this
observation
made
a
number
animals
including
humans
and
domestic
species.
Yet
pregnancy
can
be
initiated
maintained
by
using
embryo
transfer
which
bypasses
insemination
related
response.
Thus,
role
semen
exposure
beyond
sperm
transport
subsequent
tissues
yet
to
given
true
physiological
purpose.
Historically
was
suggested
remove
spermatozoa
male
derived
pathogens
from
tract.
More
recently,
have
play
long-term
preparation
maternal
immune
system
semi-allogeneic
pregnancy,
ancillary
support
preimplantation
embryo,
potentially
fetal
programing
that
improves
outcomes,
while
absence
or
inappropriate
inflammation
contribute
complications.
Although
robustly
characterized,
evidence
for
its
promoting
positive
outcomes
reducing
complications
remains
tenuous.
This
manuscript
is
designed
balance
information
we
know
regarding
various
animal
systems,
with
perceive
factual
but
perhaps
not
fully
tested,
along
data
generate
if
intend
postulate
purpose
outcomes.
