Characterization of PRDM9 Multifunctionality in Yak Testes Through Protein Interaction Mapping

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(4), С. 1420 - 1420

Опубликована: Фев. 8, 2025

Meiotic recombination is initiated by the formation of programmed DNA double-strand breaks during spermatogenesis. PRDM9 determines localization hotspots interacting with several protein complexes in mammals. The function not well understood spermatogenesis mice or yaks. In this study, we applied yeast two-hybrid assays combined next-generation sequencing techniques to screen complete set PRDM9-interacting proteins and explore its novel functions yak Our results showed that 267 were identified. gene ontology (GO) analysis revealed GO terms primarily associated spermatogenesis, positive regulation break repair via homologous recombination, RNA splicing, ubiquitin-dependent ERAD pathway, other biological processes. MKX PDCD5 verified be strongly expressed prophase I meiosis both mouse testes. localizations splicing genes including THOC5, DDX5, XRCC6 spermatocytes. Cattleyak hybrid offspring a domestic cow, male are sterile. expression was also examined sterile cattle. Among 58 detected genes, 55 downregulated cattleyak. conclusion, established interaction network, identified, which will further promote our understanding It provides new insights for study sterility.

Язык: Английский

Detecting the distribution patterns of histone lactylation in the mouse testis at different developmental stages

Gene, Год журнала: 2025, Номер unknown, С. 149355 - 149355

Опубликована: Фев. 1, 2025

Язык: Английский