ROLE OF THE NF-ΚB SIGNALING PATHWAY IN REACTIVE OXYGEN AND NITROGEN SPECIES PRODUCTION IN RAT LIVER TISSUES FOLLOWING SURGICAL TRAUMA IN AN EXPERIMENTAL MODEL OF POST-TRAUMATIC STRESS DISORDER
Актуальні проблеми сучасної медицини Вісник Української медичної стоматологічної академії,
Год журнала:
2025,
Номер
25(1), С. 139 - 144
Опубликована: Март 31, 2025
This
study
investigated
the
role
of
NF-κB
signaling
pathway
in
production
reactive
oxygen
species
(ROS)
and
nitrogen
(RNS)
rat
liver
tissues
following
surgical
trauma
an
experimental
model
post-traumatic
stress
disorder
(PTSD).
The
was
conducted
on
21
male
Wistar
rats
weighing
210–230
g.
animals
were
divided
into
three
groups:
Group
1
(Control
I)
included
intact
animals,
while
other
groups
underwent
laparotomy
against
background
a
single
prolonged
(SPS)
model,
simulating
PTSD.
For
seven
consecutive
days
surgery,
2
II)
received
daily
intraperitoneal
administration
mL
isotonic
sodium
chloride
solution
(“placebo”),
3
ammonium
pyrrolidine
dithiocarbamate
at
dose
76
mg/kg.
results
demonstrated
that
setting
SPS
leads
to
development
oxidative-nitrosative
tissues.
is
evidenced
by
increased
superoxide
anion
radical
generated
microsomal
monooxygenases,
NO
synthase,
mitochondrial
respiratory
chain,
leukocyte
NADPH
oxidase.
Additionally,
there
elevation
total
inducible
synthase
activity,
accumulation
peroxynitrite,
reduction
constitutive
activity
its
coupling
efficiency.
Pharmacological
inhibition
activation
with
contributed
liver.
limited
from
multiple
sources,
coupling,
suppressing
reducing
peroxynitrite
concentration.
Язык: Английский
LAPAROTOMY ENHANCES THE PRODUCTION OF REACTIVE NITROGEN SPECIES IN THE HEARTS OF RATS EXPOSED TO A SINGLE PROLONGED STRESS
The Medical and Ecological Problems,
Год журнала:
2023,
Номер
27(5-6), С. 26 - 30
Опубликована: Дек. 29, 2023
The
aim
of
this
study
was
to
assess
the
production
reactive
nitrogen
species
in
hearts
rats
subjected
surgical
trauma
simulated
against
background
post-traumatic
stress
disorder
(PTSD).
Materials
and
methods.The
conducted
on
42
white
Wistar
weighing
210-230
g,
then
divided
into
6
groups:
Group
1
consisted
intact
animals,
2
included
animals
exposed
induction
PTSD
model
through
single-prolonged
(SPS),
3
–
a
sham
operation,
4
undergoing
laparotomy,
5
operation
following
SPS,
laparotomy
under
modeled
SPS.
NO
synthase
activity
heart
homogenate
determined
spectrophotometrically.
Results.
formation
peroxynitrite
assessed
by
content
alkaline
alkaline-earth
metals.
SPS
exposure
significantly
increases
rats,
particular,
it
enhances
activating
inducible
isoform
reduces
constitutive
synthases,
which
is
accompanied
growth
concentration
peroxynitrites.
On
7th
day
after
experimental
model,
indicators
nitrosative
(total
concentration)
exceeded
their
values
groups
single
Язык: Английский
NITRIC OXIDE METABOLISM IN RAT SMALL INTESTINE TISSUES FOLLOWING SURGICAL TRAUMA REPRODUCED IN EXPERIMENTAL MODEL OF POSTTRAUMATIC STRESS DISORDER
Актуальні проблеми сучасної медицини Вісник Української медичної стоматологічної академії,
Год журнала:
2024,
Номер
24(1), С. 124 - 128
Опубликована: Март 20, 2024
The
article
investigates
the
parameters
of
nitric
oxide
(NO)
system
in
small
intestine
tissues
rats
subjected
to
surgical
trauma
within
an
experimental
model
post-traumatic
stress
disorder
(PTSD).
study
involved
42
white
Wistar
weighing
210-230
g,
divided
into
6
groups:
Group
1
comprised
intact
animals,
2
included
animals
with
PTSD
induced
through
single-prolonged
(SPS),
3
consisted
a
sham
operation,
4
undergoing
laparotomy,
5
operation
following
SPS,
and
laparotomy
under
modeled
SPS.
Nitric
synthase
(NOS)
activity
homogenates
was
assessed
using
spectrophotometric
methods;
peroxynitrite
formation
determined
by
analyzing
content
alkali
alkaline
earth
metals.
has
demonstrated
SPS
results
evident
signs
nitrosative
rats:
it
enhances
NOS
activating
inducible
isoform,
reduces
constitutive
coupling,
elevates
concentration
peroxynitrites
alkaline-earth
On
7th
day
modelling,
indicators
such
as
total
activity,
well
concentrations
metals,
significantly
surpassed
their
levels
single
simulated
prolonged
stress.
Язык: Английский
MORPHOLOGICAL CHARACTERISTICS OF RAT BRONCHI AGAINST THE BACKGROUND OF POST-TRAUMATIC STRESS DISORDER AND AFTER QUERCETIN CORRECTION
С. М. Білаш,
M. M. Koptev,
Олена Миколаївна Проніна
и другие.
World of Medicine and Biology,
Год журнала:
2024,
Номер
20(87), С. 182 - 182
Опубликована: Янв. 1, 2024
Язык: Английский
SOURCES OF SUPEROXIDE ANION RADICAL IN SMALL INTESTINE TISSUES IN RATS SUBJECTED TO SURGICAL TRAUMA SIMULATED UNDER EXPERIMENTAL MODEL OF POSTTRAUMATIC STRESS DISORDER
Актуальні проблеми сучасної медицини Вісник Української медичної стоматологічної академії,
Год журнала:
2024,
Номер
24(2), С. 137 - 141
Опубликована: Май 20, 2024
The
sources
of
superoxide
anion
radical
(.О
)
in
the
tissues
small
intestine
rats
subjected
to
surgical
trauma
simulated
under
an
experimental
model
posttraumatic
stress
disorder
(PTSD)
were
investigated.
study
involved
42
white
Wistar
weighing
210-230
g,
divided
into
6
groups:
group
1
comprised
intact
animals,
2
included
animals
with
PTSD
induced
through
single-prolonged
(SPS),
3
consisted
a
sham
operation,
4
undergoing
laparotomy,
5
operation
following
SPS,
and
laparotomy
modeled
SPS.
rate
.О
generation
homogenate
was
measured
spectrophotometrically
using
nitroblue
tetrazolium
test.
production
by
NADPH-dependent
(microsomal
NO
synthase)
electron
transport
chains
(ETC),
NADH-dependent
(mitochondrial)
ETC
leukocyte
NADPH
oxidase
assessed.
findings
obtained
have
demonstrated
that
SPS
modeling
leads
increase
oxidative
rats.
This
is
manifested
formation
participation
microsomes,
mitochondria
oxidase.
On
7th
day
after
model,
different
(microsomes,
oxidase)
exceeds
their
values
single
or
performing
long-term
stress.
Язык: Английский
EFFECT OF POST-TRAUMATIC STRESS DISORDER ON THE HEMOMICROCIRCULATORY BED OF RATS' LARGE BRONCHI AND ITS CORRECTION WITH QUERCETIN
С. М. Білаш,
M. M. Koptev,
Олена Миколаївна Проніна
и другие.
World of Medicine and Biology,
Год журнала:
2024,
Номер
20(88), С. 194 - 194
Опубликована: Янв. 1, 2024
Язык: Английский
COMBINED EFFECT OF LAPAROTOMY AND EXPERIMENTAL POST-TRAUMATIC STRESS DISORDER ON THE SOURCES OF SUPEROXIDE ANION RADICAL IN THE RAT HEART
The Medical and Ecological Problems,
Год журнала:
2024,
Номер
28(2), С. 29 - 34
Опубликована: Июль 15, 2024
The
aim
of
this
study
was
to
determine
the
sources
superoxide
anion
radical
(O₂⁻•)
in
rat
heart
homogenate
under
conditions
surgical
trauma
inflicted
against
background
an
experimental
model
posttraumatic
stress
disorder
(PTSD).
Materials
and
methods.
experiments
were
conducted
on
42
white
Wistar
rats
weighing
(220±10)
g,
divided
into
6
groups:
Group
1
–
intact
animals;
2
rodents
with
PTSD
modeled
by
reproducing
single
prolonged
(SPS);
3
that
underwent
a
sham
operation;
4
animals
laparotomy;
5
operation
after
SPS
modeling;
laparotomy
SPS.
rate
O₂⁻•
generation
studied
using
spectrophotometric
test
nitroblue
tetrazolium.
Results.
modeling
(SPS)
leads
significant
increase
oxidative
heart,
evidenced
elevated
involving
NADPH-dependent
electron
transport
chains
(ETC)
microsomes,
NO
synthase,
mitochondrial
respiratory
chain,
leukocyte
NADPH
oxidase.
In
post-laparotomy
period
following
SPS,
enhanced
significantly
manifested
through
increased
microsomal
synthase
ETC
72.3%,
NADH-dependent
72.9%,
oxidase
80.9%
compared
control.
When
group
2,
these
indicators
rose
29.6%,
24.4%,
26.8%,
respectively.
demonstrated
even
more
pronounced
generation,
reaching
54.0%,
54.6%,
62.9%
Furthermore,
production
rates
from
all
likely
higher
than
those
29.8%,
21.2%,
21.5%
,
Conclusions.
combined
effect
synergistically
enhances
various
sources,
exceeding
levels
observed
either
or
alone.
Язык: Английский
MARKERS OF CONNECTIVE TISSUE DEGRADATION IN THE SMALL INTESTINE OF RATS UNDERGOING SURGICAL TRAUMA REPLICATED UNDER EXPERIMENTAL POST-TRAUMATIC STRESS DISORDER
Актуальні проблеми сучасної медицини Вісник Української медичної стоматологічної академії,
Год журнала:
2023,
Номер
23(4), С. 244 - 247
Опубликована: Дек. 20, 2023
The
objective
of
this
experiment
is
to
study
the
indices
depolymerisation
biopolymers
in
small
intestine
connective
tissue
rats
undergoing
surgical
trauma
replicated
under
post-traumatic
stress
disorder.
was
carried
out
on
42
white
rats.
findings
obtained
have
shown
that
experimental
modelling
posttraumatic
disorder
(single
prolonged
stress)
significantly
elevates
concentration
free
hydroxyproline
(by
44.9%,
P<0.
001),
hexuronic
acids
47.0%,
P<0.001),
and
N-acetylneuraminic
acid
46.2%,
indicating
extracellular
matrix
components,
collagen,
proteoglycans,
sialoglycoproteins,
organ.
On
7th
day
after
laparotomy
performed
model
disorder,
content
hydroxyproline,
acids,
homogenate
exceeded
respective
values
separate
"sham"
operation
simulated
single
stress.
Язык: Английский