Expression patterns of MCM8 in lung adenocarcinoma and its correlation with key biological processes
European journal of medical research,
Год журнала:
2025,
Номер
30(1)
Опубликована: Март 3, 2025
Lung
adenocarcinoma
(LUAD)
is
one
of
the
most
common
and
lethal
tumors.
The
identification
diagnostic
prognostic
biomarkers
essential
to
improve
patient
prognosis
treatment
outcomes.
expression
minichromosome
maintenance
complex
component
8
(MCM8)
in
33
cancer
types
was
analyzed
using
Cancer
Genome
Atlas
(TCGA)
Genotype-Tissue
Expression.
Tumor
normal
tissues
LUAD
were
compared
TCGA
data
validated
against
four
datasets
from
Gene
Expression
Omnibus.
MCM8
assessed
by
immunohistochemistry
(IHC)
tissue
microarrays.
value
Receiver
Operating
Characteristic
curve
analysis,
its
significance
determined
Kaplan–Meier
analysis.
CIBERSORT
method
used
examine
immune
infiltration.
association
between
m6A
RNA
methylation,
glycolysis,
ferroptosis
GEPIA
online
tool.
markedly
overexpressed
many
tumors
including
LUAD.
showed
high
accuracy
for
diagnosis
LUAD,
with
an
area
under
0.849
dataset.
overexpression
tumor
confirmed
IHC
shown
be
associated
decreased
overall
survival
disease-specific
survival.
Analysis
cell
infiltration
that
populations
differed
low
groups.
correlated
genes
ferroptosis.
identified
as
a
promising
marker
mechanism
underlying
effect
on
development
response
remains
elucidated.
Язык: Английский
Exploration of telomere-related biomarkers for lung adenocarcinoma and targeted drug prediction
Discover Oncology,
Год журнала:
2025,
Номер
16(1)
Опубликована: Фев. 10, 2025
Bioinformatics
analyses
were
performed
to
identify
telomere
biomarkers
develop
a
diagnostic
model
for
lung
adenocarcinoma
(LUAD)
and
predict
potential
target
drugs
patients
with
LUAD.
Telomeres
function
crucially
in
maintaining
genome
stability
chromosome
integrity,
telomere-related
genes
(TRGs)
serve
as
prognostic
markers
variety
of
cancers.
However,
studies
focusing
on
TRGs
LUAD
are
limited.
To
screen
key
evaluate
their
impact
the
occurrence
development
samples
collected
from
University
California
Santa
Cruz
(UCSC)
Xena
2093
obtained
TelNet
database.
Hub
screened
using
"WGCNA"
package.
Differentially
expressed
(DEGs)
between
tumor
control
filtered
"DESeq"
Protein–protein
interaction
(PPI)
network
analysis
was
select
candidate
genes,
which
identified
by
machine
learning
used
nomogram.
Functional
enrichment
pathways
analyzed
"clusterProfiler"
Correlation
immune
cell
infiltration
examined
Spearman
method.
Targeted
predicted
molecular
docking
models
developed
AutoDockTools.
Finally,
validated
performing
vitro
cellular
assays.
A
total
259
hub
2848
DEGs,
48
differentially
screened.
Subsequently,
13
PPI
analysis.
LASSO
support
vector
machine-recursive
feature
elimination
(SVM-RFE)
algorithms
further
reduced
number
four
(CCNB1,
CDC20,
PLK1,
TOP2A).
nomogram
strong
predictive
performance
created.
These
mainly
enriched
mitogenic
exhibited
correlation
infiltration.
Three
(Lucanthone,
Fulvestrant,
Myricetin)
targeting
be
able
treat
experiments
demonstrated
that
CCNB1
PLK1
have
effects
proliferation,
migration,
invasion
AKT/mTOR
signaling
pathway
cells.
This
study
provided
novel
biomarkers,
therapeutic
targets,
Язык: Английский
Integrated genomic analysis of the stemness index signature of mRNA expression predicts lung adenocarcinoma prognosis and immune landscape
PeerJ,
Год журнала:
2025,
Номер
13, С. e18945 - e18945
Опубликована: Фев. 13, 2025
mRNA
expression-based
stemness
index
(mRNAsi)
has
been
used
for
prognostic
assessment
in
various
cancers,
but
its
application
lung
adenocarcinoma
(LUAD)
is
limited,
which
the
focus
of
this
study.
Low
mRNAsi
LUAD
predicted
a
better
prognosis.
Eight
genes
(
GNG7,
EIF5A,
ANLN,
FKBP4,
GAPDH,
GNPNAT1,
E2F7,
CISH
)
associated
with
were
screened
to
establish
risk
model.
The
differentially
expressed
between
high
and
low
groups
mainly
enriched
metabolism,
cell
cycle
functions
pathway.
score
group
had
higher
immune
scores.
Patients
lower
TIDE
scores
immunotherapy
outcomes.
In
addition,
was
effective
assessing
drug
sensitivity
LUAD.
Reverse
transcription-quantitative
polymerase
chain
reaction
(RT-qPCR)
data
showed
that
eight
lines,
knockdown
EIF5A
reduced
invasion
migration
ability
cells.
This
study
designed
model
based
on
mRNAsi-related
predicting
accurately
prognosis
survival
patients,
facilitating
patients
chemotherapy.
Язык: Английский
Bioinformatic methods uncover 5 diagnostic biomarkers associated with drug resistance and metastasis for gastrointestinal stromal tumor
Fang Fang,
Renzhong Tai,
Yang Fu
и другие.
Current Pharmaceutical Analysis,
Год журнала:
2025,
Номер
21(2), С. 67 - 76
Опубликована: Фев. 1, 2025
Язык: Английский
A nicotinamide metabolism-related gene signature for predicting immunotherapy response and prognosis in lung adenocarcinoma patients
PeerJ,
Год журнала:
2025,
Номер
13, С. e18991 - e18991
Опубликована: Фев. 27, 2025
Background
Nicotinamide
(NAM)
metabolism
fulfills
crucial
functions
in
tumor
progression.
The
present
study
aims
to
establish
a
NAM
metabolism-correlated
gene
(NMRG)
signature
assess
the
immunotherapy
response
and
prognosis
of
lung
adenocarcinoma
(LUAD).
Methods
training
set
validation
(the
GSE31210
dataset)
were
collected
Cancer
Genome
Atlas
(TCGA)
Gene
Expression
Omnibus
(GEO),
respectively.
Molecular
subtypes
LUAD
classified
by
consensus
clustering.
Mutation
landscape
top
20
somatic
genes
was
visualized
maftools
package.
Subsequently,
differential
expression
analysis
conducted
using
limma
package,
univariate,
multivariate
LASSO
regression
analyses
performed
on
screened
construct
risk
model
for
LUAD.
Next,
MCP-counter,
TIMER
ESTIMATE
algorithms
utilized
comprehensively
immune
microenvironmental
profile
patients
different
groups.
efficacy
chemotherapy
drugs
evaluated
TIDE
score
pRRophetic
A
nomogram
created
integrating
RiskScore
clinical
features.
mRNA
expressions
independent
prognostic
NMRGs
migration
invasion
cells
measured
carrying
out
cellular
assays.
Results
Two
(C1
C2)
classified,
with
C1
subtype
showing
worse
than
C2.
three
high
mutation
frequency
C2
TTN
(45.25%),
FLG
(25.25%),
ZNF536
(19.8%).
Four
(
GJB3
,
CPA3
DKK1
KRT6A
)
used
model,
which
exhibited
strong
predictive
performance.
High-risk
group
showed
low
cell
infiltration,
score,
prognosis,
this
drug
sensitivity
Cisplatin,
Erlotinib,
Paclitaxel,
Saracatini,
CGP_082996.
established
an
accurate
diagnostic
all
high-
expressed
cells,
silencing
inhibited
cells.
Conclusion
novel
NMRG
developed,
contributing
evaluation
personalized
treatment
patients.
Язык: Английский
Development of a reliable risk prognostic model for lung adenocarcinoma based on the genes related to endotheliocyte senescence
Scientific Reports,
Год журнала:
2025,
Номер
15(1)
Опубликована: Апрель 12, 2025
Cellular
senescence
is
a
hallmark
for
cancers,
particularly
in
lung
adenocarcinoma
(LUAD).
This
study
developed
risk
model
using
signature
genes
LUAD
patients.
Based
on
the
RNA-seq,
clinical
information
and
mutation
data
of
patients
collected
from
TCGA
GEO
database,
we
obtained
102
endotheliocyte
senescence-related
genes.
The
"ConsensusClusterPlus"
R
package
was
employed
unsupervised
cluster
analysis,
"limma"
used
differentially
expressed
gene
(DEG)
analysis.
A
prognosis
created
by
univariate
multivariate
Cox
regression
analysis
combined
with
Lasso
utilizing
"survival"
"glmnet"
packages.
KM
survival
receiver
operator
characteristic
curve
analyses
were
conducted
applying
"timeROC"
"MCPcounter"
immune
infiltration
Immunotherapy
response
performed
based
IMvigor210
GSE78220
cohort,
drug
sensitivity
predicted
"pRRophetic"
package.
Cell
invasion
migration
tested
carrying
out
Transwell
wound
healing
assays.
According
to
results,
total
32
related
screened
assign
into
C1
C2
subtypes.
subtype
showed
significantly
worse
an
overall
higher
somatic
frequency,
which
associated
increased
activation
cancer
pathways,
including
Myc_targets2
angiogenesis.
Then,
DEGs
between
two
subtypes,
constructed
five-gene
RiskScore
strong
classification
effectiveness
short-
long-term
OS
prediction.
High-
low-risk
groups
classified
RiskScore.
High-risk
patients,
characterized
lower
infiltration,
had
poorer
outcomes
both
training
validation
datasets.
immunotherapy
LUAD.
Finally,
found
that
potential
drugs
such
as
Cisplatin
can
benefit
high-risk
In-vitro
experiments
demonstrated
silencing
Angiopoietin-like
4
(ANGPTL4),
Gap
Junction
Protein
Beta
3
(GJB3),
Family
sequence
similarity
83-member
(FAM83A),
Anillin
(ANLN)
reduced
number
invasive
cells
rate,
while
solute
carrier
family
34
member
2
(SLC34A2)
opposite
effect.
study,
collectively
speaking,
facilitate
diagnosis
treatment
Язык: Английский
The cancer stem cells characteristics analysis of LGR5 + cells that influence lung cancer risk by using single cell RNA-seq analysis
Scientific Reports,
Год журнала:
2025,
Номер
15(1)
Опубликована: Май 8, 2025
Lung
adenocarcinoma
(LUAD)
is
the
most
popular
lung
cancer
type
with
highly
mortality.
We
performed
a
single
cell
RNA-seq
analysis
to
explore
characteristic
of
stem
cells
in
LUAD.
downloaded
data
(GSE149655)
from
GEO
database,
scRNA-seq
was
by
using
"Seurat"
and
"harmony"
R
package.
The
FindMarkers
function
"ClusterProlifer"
package
used
for
differentially
expressed
genes
(DEGs)
enrichment
analysis.
protein-protein
interaction
transcriptional
regulatory
network
were
STRING
ChIPBase
database.
Immunohistochemistry
tests
be
observe
differences
expression
specific
LUAD
paracancerous
tissue
samples.
BEAS-2B
A549
vitro
assay
qRT-PCR,
western
blotting,
wound
healing,
trans-well
assays,
EdU
tests,
flow
cytometry
performed.
A
total
9
clusters
obtained
after
analysis,
which
had
higher
proportion
Subsequently,
revealed
that
amino
sugar
nucleotide
metabolism
DNA
replication
pathways
activated
(CSCs),
further
sub-divided
into
3
subtypes,
LGR5
+
major
contributor
progression,
its
hub
genes,
such
as
HLA-DPB1,
CD74,
CTSH
HLA-DRB5
mediated
unique
state.
In
addition,
marker
three
CSCs
also
overexpressed
CXCL3
played
an
important
role
mediating
proliferation,
apoptosis,
migration
invasion
tumor.
identified
our
findings
are
expected
provide
new
insights
pathogenesis
Язык: Английский
A diagnostic signature developed based on the necroptosis-related genes and its association with immune infiltration in osteosarcoma
Heliyon,
Год журнала:
2024,
Номер
10(16), С. e35719 - e35719
Опубликована: Авг. 1, 2024
Osteosarcoma
is
a
bone-derived
malignancy
that
often
leads
to
lung
metastasis
and
death.
Язык: Английский
Analysis on the involvement of phosphoglycerate mutase 1 in the aerobic glycolysis of melanoma cells
International Journal of Biological Macromolecules,
Год журнала:
2024,
Номер
unknown, С. 137898 - 137898
Опубликована: Ноя. 1, 2024
Язык: Английский
Retrospective analysis of clinical and molecular characteristics as prognostic factors in adult T-cell lymphoblastic lymphoma
American Journal of Cancer Research,
Год журнала:
2024,
Номер
14(12), С. 5851 - 5862
Опубликована: Янв. 1, 2024
To
analyze
the
clinical
characteristics
and
molecular
biomarkers
of
adult
T-cell
lymphoblastic
lymphoma
(T-LBL)
to
identify
prognostic
factors,
evaluate
efficacy
different
chemotherapy
regimens,
providing
a
basis
for
optimizing
treatment
strategies
T-LBL.
A
total
89
Patients
aged
18-72
years
with
T-LBL,
confirmed
via
histopathological
examination
lymph
nodes,
extranodal
tissues,
or
bone
marrow,
were
retrospectively
included.
Clinical
data,
details,
mutational
profiles
collected.
Prognostic
factors
assessed
based
on
characteristics,
safety
two
regimens
compared.
Descriptive
statistics
used
disease
spectrum.
Most
patients
(84.00%)
presented
advanced
(stages
III-IV).
Mediastinal
invasion
was
observed
in
63
(70.80%),
59
(66.30%)
exhibited
B
symptoms.
Bone
marrow
involvement
occurred
19
(21.20%),
bulky
mediastinum
(>10
cm)
present
50
(56.18%).
Mutations
detected
29
patients,
NOTCH1
being
most
frequently
mutated
gene,
followed
by
PHF-6,
JAK-1,
JAK-3,
IL-7R,
TP53.
The
complete
response
(CR)
rate
51.69%.
3-year
overall
survival
(OS)
progression-free
(PFS)
rates
74.9%
58.80%,
respectively.
Multivariate
analysis
identified
female
sex,
lack
CR,
elevated
lactate
dehydrogenase
(LDH)
levels
(>2×
normal)
as
independent
predictors
poor
OS
(58.25%).
Chemotherapy
LDH
levels,
sex
PFS
(21.24%).
T-LBL
is
characterized
high-frequency
gene
mutations
across
multiple
signaling
pathways.
(70.80%)
(39.33%)
prevalent
Chinese
associated
prognosis.
Combined
assessment
features
allows
improved
stratification
facilitates
development
targeted
therapies
high-risk
patients.
Язык: Английский