Mitigation of cyclophosphamide-induced premature ovarian insufficiency in rats through bone marrow-derived mesenchymal stem cells and placental extract therapy: a comprehensive evaluation DOI Creative Commons
Ahmed Atwa,

Nehal Sobhy,

Abdel Kareem M. Abdel Latif

и другие.

Beni-Suef University Journal of Basic and Applied Sciences, Год журнала: 2025, Номер 14(1)

Опубликована: Фев. 11, 2025

Abstract Objective Premature ovarian insufficiency (POI) is a significant reproductive health concern characterized by the depletion of primary follicles, often resulting from exposure to genotoxic agents. This study aimed explore therapeutic potential bone marrow-derived mesenchymal stem cells (BM-MSCs) and/or placental extracts (PE) in ameliorating POI induced cyclophosphamide, widely used chemotherapeutic agent. Materials and methods Thirty-five 8–10 weeks weight 200 ± 20 g female albino Sprague–Dawley (SD) rats were evenly distributed into five groups: negative control (Group 1), positive cyclophosphamide (CPA) 2), cell therapy (CPA+MSCs) 3), extract (CPA+PE) 4), combination (CPA+MSCs+PE) 5). Rats CPA group received intraperitoneal (IP) injection (50 mg/kg), followed daily (8 mg/kg) (14 days). Subsequently, 1 × 10 6 MSCs via intravenous administration 50 µL PE, sacrifice after 4 weeks. Flow cytometry was identify MSCs. Hormone levels (FSH, LH, E 2 , progesterone) assessed using ELISA, primordial follicles quantified evaluate follicle reserves. Ovarian structure histomorphologically evaluated, PCNA immunohistochemistry conducted. Morphometric measurements statistical analyses performed. Results Hormonal revealed decrease E2 progesterone levels, accompanied an increase FSH following treatment. However, BM-MSC therapy, hormonal nearly returned normal. In addition PE treatment also evaluated. resulted partial restoration balance, showing mild with slight reduction compared cyclophosphamide-treated group. Histological examination that caused loss stromal blood vessel damage, substantial fibrosis. Interestingly, showed some ameliorative effects on histology, reduced fibrosis preservation although these changes less pronounced than those observed alone. Conclusion more effective restoring folliculogenesis, whereas provided moderate protection against histological immunohistochemical alterations cyclophosphamide.

Язык: Английский

Mitigation of cyclophosphamide-induced premature ovarian insufficiency in rats through bone marrow-derived mesenchymal stem cells and placental extract therapy: a comprehensive evaluation DOI Creative Commons
Ahmed Atwa,

Nehal Sobhy,

Abdel Kareem M. Abdel Latif

и другие.

Beni-Suef University Journal of Basic and Applied Sciences, Год журнала: 2025, Номер 14(1)

Опубликована: Фев. 11, 2025

Abstract Objective Premature ovarian insufficiency (POI) is a significant reproductive health concern characterized by the depletion of primary follicles, often resulting from exposure to genotoxic agents. This study aimed explore therapeutic potential bone marrow-derived mesenchymal stem cells (BM-MSCs) and/or placental extracts (PE) in ameliorating POI induced cyclophosphamide, widely used chemotherapeutic agent. Materials and methods Thirty-five 8–10 weeks weight 200 ± 20 g female albino Sprague–Dawley (SD) rats were evenly distributed into five groups: negative control (Group 1), positive cyclophosphamide (CPA) 2), cell therapy (CPA+MSCs) 3), extract (CPA+PE) 4), combination (CPA+MSCs+PE) 5). Rats CPA group received intraperitoneal (IP) injection (50 mg/kg), followed daily (8 mg/kg) (14 days). Subsequently, 1 × 10 6 MSCs via intravenous administration 50 µL PE, sacrifice after 4 weeks. Flow cytometry was identify MSCs. Hormone levels (FSH, LH, E 2 , progesterone) assessed using ELISA, primordial follicles quantified evaluate follicle reserves. Ovarian structure histomorphologically evaluated, PCNA immunohistochemistry conducted. Morphometric measurements statistical analyses performed. Results Hormonal revealed decrease E2 progesterone levels, accompanied an increase FSH following treatment. However, BM-MSC therapy, hormonal nearly returned normal. In addition PE treatment also evaluated. resulted partial restoration balance, showing mild with slight reduction compared cyclophosphamide-treated group. Histological examination that caused loss stromal blood vessel damage, substantial fibrosis. Interestingly, showed some ameliorative effects on histology, reduced fibrosis preservation although these changes less pronounced than those observed alone. Conclusion more effective restoring folliculogenesis, whereas provided moderate protection against histological immunohistochemical alterations cyclophosphamide.

Язык: Английский

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