Wheat COBRA-like Gene TaCOBL6A2 Confers Heat Tolerance in Plants DOI Open Access

Qingyan Deng,

Jiangtao Luo,

Jianmin Zheng

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(9), С. 4101 - 4101

Опубликована: Апрель 25, 2025

Wheat, a cold-tolerant crop, suffers substantial yield and quality losses under heat stress, yet the genetic mechanisms underlying thermotolerance remain understudied. We characterized TaCOBL6A2, novel COBRA-like gene on wheat chromosome 6A encoding glycosylphosphatidylinositol (GPI)-anchored protein with conserved COBRA domain, performed subcellular localization, tissue-specific expression, stress response analyses to investigate its function. Functional validation was conducted based TaCOBL6A2 overexpression in Arabidopsis transcriptomic profiling. Additionally, haplotype analysis of varieties associate genotypes phenotypes. The results show that is localized plasma membrane, cell wall, nucleus, highest expression early-stage grains. Its transcription strongly induced by exceeding cold, salt, or drought. enhanced activated shock proteins (HSPs) oxygen homeostasis pathways. elite haplotype, Hap1, associated improved seedling growth elevated antioxidant enzyme activity stress. Our findings reveal key regulator tolerance could be used as molecular target for breeding climate-resilient cultivars.

Язык: Английский

Wheat COBRA-like Gene TaCOBL6A2 Confers Heat Tolerance in Plants DOI Open Access

Qingyan Deng,

Jiangtao Luo,

Jianmin Zheng

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(9), С. 4101 - 4101

Опубликована: Апрель 25, 2025

Wheat, a cold-tolerant crop, suffers substantial yield and quality losses under heat stress, yet the genetic mechanisms underlying thermotolerance remain understudied. We characterized TaCOBL6A2, novel COBRA-like gene on wheat chromosome 6A encoding glycosylphosphatidylinositol (GPI)-anchored protein with conserved COBRA domain, performed subcellular localization, tissue-specific expression, stress response analyses to investigate its function. Functional validation was conducted based TaCOBL6A2 overexpression in Arabidopsis transcriptomic profiling. Additionally, haplotype analysis of varieties associate genotypes phenotypes. The results show that is localized plasma membrane, cell wall, nucleus, highest expression early-stage grains. Its transcription strongly induced by exceeding cold, salt, or drought. enhanced activated shock proteins (HSPs) oxygen homeostasis pathways. elite haplotype, Hap1, associated improved seedling growth elevated antioxidant enzyme activity stress. Our findings reveal key regulator tolerance could be used as molecular target for breeding climate-resilient cultivars.

Язык: Английский

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