Reversal of Diabetic Dry Eye by Topical Opioid Receptor Blockade Follows Dual Pathways DOI Creative Commons
David R. Diaz, Joseph W. Sassani,

Ian S. Zagon

и другие.

Investigative Ophthalmology & Visual Science, Год журнала: 2025, Номер 66(3), С. 24 - 24

Опубликована: Март 10, 2025

Purpose: To determine pathways in the trigeminal ganglion and corneal epithelium that are targeted by topical naltrexone (NTX) treatment for dry eye. Methods: NTX drops were administered topically daily 15 days to surface of male female adult type 1 diabetic rats. Schirmer scores sensitivity measured at baseline, 5, 10, days. Trigeminal processed immunohistochemistry detect expression opioid growth factor receptor (OGFr), Ki67, nerve factor, insulin-like factor-1, calcitonin gene-related peptide, substance P, TNF-α. A proteomic study determined protein changes cornea. Results: Corneal tear production rats restored normal levels within 5 after NTX. Assessment tissue revealed defects OGFr expression, epithelial cell number, Ki67+ Inflammation markers (e.g., TNF-α) reduced from treated with Proteomic data suggest diabetes causes dysregulation inflammatory biological processes. The percentages peptide–positive neurons, but not P–positive increased treatment. Diabetic responded a comparable manner. Conclusions: Type results decreased altered sensitivity. These complications coincide dysregulated maintains ocular homeostasis. Reversal eye restoration occur following dual cellular proliferation reduction inflammation.

Язык: Английский

Reversal of Diabetic Dry Eye by Topical Opioid Receptor Blockade Follows Dual Pathways DOI Creative Commons
David R. Diaz, Joseph W. Sassani,

Ian S. Zagon

и другие.

Investigative Ophthalmology & Visual Science, Год журнала: 2025, Номер 66(3), С. 24 - 24

Опубликована: Март 10, 2025

Purpose: To determine pathways in the trigeminal ganglion and corneal epithelium that are targeted by topical naltrexone (NTX) treatment for dry eye. Methods: NTX drops were administered topically daily 15 days to surface of male female adult type 1 diabetic rats. Schirmer scores sensitivity measured at baseline, 5, 10, days. Trigeminal processed immunohistochemistry detect expression opioid growth factor receptor (OGFr), Ki67, nerve factor, insulin-like factor-1, calcitonin gene-related peptide, substance P, TNF-α. A proteomic study determined protein changes cornea. Results: Corneal tear production rats restored normal levels within 5 after NTX. Assessment tissue revealed defects OGFr expression, epithelial cell number, Ki67+ Inflammation markers (e.g., TNF-α) reduced from treated with Proteomic data suggest diabetes causes dysregulation inflammatory biological processes. The percentages peptide–positive neurons, but not P–positive increased treatment. Diabetic responded a comparable manner. Conclusions: Type results decreased altered sensitivity. These complications coincide dysregulated maintains ocular homeostasis. Reversal eye restoration occur following dual cellular proliferation reduction inflammation.

Язык: Английский

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