Sorlinia euscelidii gen. nov., sp. nov., a novel acetic acid bacterium isolated from the leafhopper Euscelidius variegatus (Hemiptera: Cicadellidae) DOI
Ramona Marasco, Grégoire Michoud, Kholoud A. Seferji

и другие.

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Год журнала: 2024, Номер 74(10)

Опубликована: Окт. 21, 2024

Acetic acid bacteria – belonging to the Acetobacteraceae family are found in gut of many sugar-feeding insects. In this study, six strains have been isolated from hemipteran leafhopper Euscelidius variegatus . While they exhibit high 16S rRNA gene sequence similarities uncultured members family, could not be unequivocally assigned any particular type species. Considering clonality isolates, EV16P T strain was used as a representative group isolates. The genome is composed 2.388 Mbp chromosome, with DNA G+C content 57 mol%. Phylogenetic analyses based on and whole-genome multilocus analysis indicate that forms monophyletic clade uncultivated endosymbiont Diaphorina citri , Candidatus Kirkpatrickella diaphorinae. Such phylogenetic positioned between those Asaia-Swaminathania Kozakia genomic distance metrics protein sequences support proposal new species yet-undescribed genus. It rod-shaped Gram-stain-negative bacterium, strictly aerobic, non-motile, non-spore-forming, showing optimal growth without salt (NaCl) at 30 °C pH 6–7. major quinone Q10, dominant cellular fatty acids (>10%) C 18:l ω 7c, 19 : 0 cyclo 6c, 16 1 2OH. polar lipid profile comprises diphosphatidylglycerol, phosphatidylethanolamine phosphatidylcholine, along unidentified aminophospholipids, glycophospholipids, aminolipids lipids. Based polyphasic approach, including phylogenetic, phylogenomic, relatedness, phenotypic chemotaxonomic characterisations, (= KCTC 8296 = DSM 117028 ) proposed novel genus name Sorlinia euscelidii gen. nov., sp. honour Prof. Claudia Sorlini, an Italian environmental microbiologist University Milan who inspired research microbial diversity, symbiosis plants animals.

Язык: Английский

Improved detection of fungi and uncultivated microorganisms in soil metagenomes using a comprehensive genome database DOI Creative Commons
Zoey R. Werbin, Ilija Dukovski, Dylan Mankel

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Март 25, 2025

Abstract Soils harbor diverse microbial communities crucial for ecosystem functioning, but poor genomic representation of many uncultured soil microorganisms limits the utility existing databases to address some most pressing questions in environmental microbiology. To this, we developed SoilMicrobeDB, a comprehensive, genome-based reference database enhance metagenomic classification ecosystems, with focus on previously underrepresented fungal taxa and organisms. We evaluated using large metagenome dataset, comparing rates, analyzing fungal-bacterial ratios against phospholipid fatty acid (PLFA) estimates, validating lineage abundances rRNA amplicon sequencing data. Mock community analysis was also conducted test precision prevalence false positives. The SoilMicrobeDB workflow improved read by over 20% provided more accurate abundance particularly nutrient cycling groups such as ectomycorrhizal fungi. Metagenomic-derived were correlated PLFA qPCR proportions aligned relative estimates from sequencing. Uncultured represented up 50% classifiable certain biomes. offers robust taxonomic functional profiling provides scalable updatable tool ecology research. is accessible through an interactive platform linking genomes factors, enabling researchers explore distributions across conditions potentially leading new insights into management practices.

Язык: Английский

Процитировано

0
Modelling soil prokaryotic traits across environments with the trait sequence database ampliconTraits and the R package MicEnvMod DOI Creative Commons
Jonathan Donhauser, Anna Doménech‐Pascual, Xingguo Han

и другие.

Ecological Informatics, Год журнала: 2024, Номер unknown, С. 102817 - 102817

Опубликована: Сен. 1, 2024

Язык: Английский

Процитировано

3
SeqCode in the golden age of prokaryotic systematics DOI Creative Commons
Diego Javier Jiménez, Alexandre Soares Rosado

The ISME Journal, Год журнала: 2024, Номер 18(1)

Опубликована: Янв. 1, 2024

Abstract The SeqCode is a new code of prokaryotic nomenclature that was developed to validate taxon names using genome sequences as the type material. present article provides an independent view about SeqCode, highlighting its history, current status, basic features, pros and cons, use date. We also discuss important topics consider for validation novel genomes Owing significant advances in metagenomics cultivation methods, hundreds species are expected be discovered coming years. This manuscript aims stimulate enrich debate around upcoming golden age discovery systematics.

Язык: Английский

Процитировано

2
An overview of Parafrankia (Nod+/Fix+) and Pseudofrankia (Nod+/Fix−) interactions through genome mining and experimental modeling in co-culture and co-inoculation of Elaeagnus angustifolia DOI
Maher Gtari,

Nicholas Beauchemin,

Indrani Sarker

и другие.

Applied and Environmental Microbiology, Год журнала: 2024, Номер 90(5)

Опубликована: Апрель 23, 2024

In many frankia, the ability to nodulate host plants (Nod+) and fix nitrogen (Fix+) is a common strategy. However, some frankia within

Язык: Английский

Процитировано

2
Sorlinia euscelidii gen. nov., sp. nov., a novel acetic acid bacterium isolated from the leafhopper Euscelidius variegatus (Hemiptera: Cicadellidae) DOI
Ramona Marasco, Grégoire Michoud, Kholoud A. Seferji

и другие.

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Год журнала: 2024, Номер 74(10)

Опубликована: Окт. 21, 2024

Acetic acid bacteria – belonging to the Acetobacteraceae family are found in gut of many sugar-feeding insects. In this study, six strains have been isolated from hemipteran leafhopper Euscelidius variegatus . While they exhibit high 16S rRNA gene sequence similarities uncultured members family, could not be unequivocally assigned any particular type species. Considering clonality isolates, EV16P T strain was used as a representative group isolates. The genome is composed 2.388 Mbp chromosome, with DNA G+C content 57 mol%. Phylogenetic analyses based on and whole-genome multilocus analysis indicate that forms monophyletic clade uncultivated endosymbiont Diaphorina citri , Candidatus Kirkpatrickella diaphorinae. Such phylogenetic positioned between those Asaia-Swaminathania Kozakia genomic distance metrics protein sequences support proposal new species yet-undescribed genus. It rod-shaped Gram-stain-negative bacterium, strictly aerobic, non-motile, non-spore-forming, showing optimal growth without salt (NaCl) at 30 °C pH 6–7. major quinone Q10, dominant cellular fatty acids (>10%) C 18:l ω 7c, 19 : 0 cyclo 6c, 16 1 2OH. polar lipid profile comprises diphosphatidylglycerol, phosphatidylethanolamine phosphatidylcholine, along unidentified aminophospholipids, glycophospholipids, aminolipids lipids. Based polyphasic approach, including phylogenetic, phylogenomic, relatedness, phenotypic chemotaxonomic characterisations, (= KCTC 8296 = DSM 117028 ) proposed novel genus name Sorlinia euscelidii gen. nov., sp. honour Prof. Claudia Sorlini, an Italian environmental microbiologist University Milan who inspired research microbial diversity, symbiosis plants animals.

Язык: Английский

Процитировано

2